|本期目录/Table of Contents|

[1]王丽,王垂杰,孙晓婷,等.慢性浅表性胃炎大鼠胃黏膜细胞凋亡与中医证型的相关性[J].环球中医药,2013,6(06):401-0.
　WANG Li,WANG Chui jie,SUN Xiao ting..Correlation between apoptosis of gastric mucosa with Chinese medical pattern in rats with chronic superficial gastritis[J].,2013,6(06):401-0.
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慢性浅表性胃炎大鼠胃黏膜细胞凋亡与中医证型的相关性()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第6卷
期数:
2013年06期
页码:
401-0
栏目:
论著
出版日期:
2013-06-06

文章信息/Info

Title:
Correlation between apoptosis of gastric mucosa with Chinese medical pattern in rats with chronic superficial gastritis
作者:
王丽;王垂杰;孙晓婷;
Author(s):
WANG Li;  WANG Chuijie;  SUN Xiaoting.
Nursing College of Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China
关键词:
脾虚证; 湿热证; 细胞凋亡; B细胞淋巴瘤/白血病-2基因
Keywords:
Spleendeficiency pattern; Dampheat pattern; Apoptosis; Bcl2 mRNA
分类号:
R5733+1
DOI:
-
文献标志码:
A
摘要:
目的探讨慢性浅表性胃炎(chronicsuperficialgastritis,CSG)大鼠不同证型胃黏膜细胞凋亡及其调控基因B细胞淋巴瘤/白血病-2(B-celllym-phoma/Leukemia-2,Bcl-2)mRNA表达水平的变化,从细胞凋亡的角度揭示CSG各证候的实质。方法复制大鼠单纯CSG模型、脾虚CSG和湿热CSG模型,检测胃黏膜上皮细胞凋亡指数及其调控基因Bcl-2mRNA表达水平。结果三个模型组大鼠胃黏膜细胞凋亡指数均显著高于正常对照组,以脾虚CSG组最高。三个模型组较正常组Bcl-2mRNA表达均降低,以脾虚CSG组最低。结论CSG大鼠胃黏膜细胞凋亡指数、Bcl-2mRNA表达水平与CSG证候有一定的相关性,以脾虚CSG组大鼠胃黏膜细胞凋亡指数最高和Bcl-2mRNA表达水平最低。
Abstract:
ObjectiveTo explore pattern essence from the aspect of apoptosis by observing apoptosis of gastric mucosa and expression of regulatory gene Bcl2 mRNA in chronic superficial gastritis rats with different Chinese medical patterns. MethodsCSG rat models, CSG rat models with spleendeficiency pattern and dampheat pattern were copied respectively. Apoptosis of gastric mucosa and expression of Bcl2 mRNA were detected. ResultsApoptosis indexes of gastric mucosa in model groups, especially in CSG with spleendeficiency group, were higher than those in normal control group, While the expression of Bcl2 mRNA were lower than those in normal control group. ConclusionThere is correlation between apoptosis index of gastric mucosa and expression of Bcl2 mRNA with Chinese medical patterns in CSG rats. Apoptosis index of gastric mucosa in CSG with spleendeficiency group is the highest, and the expression of Bcl2 mRNA is the lowest.

参考文献/References:

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［2］高碧珍,兰启防,李灿东,等.慢性胃炎证候与幽门螺杆菌及Bcl2蛋白的相关性研究.福建中医学院学报.2003,12,13(6):13.
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［5］宋雅芳,王汝俊,刘友章,等.健脾益气中药对脾虚大鼠肝组织线粒体功能的影响［J］.中药新药与临床药理,2009,20(5):423426.
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备注/Memo

备注/Memo:

作者单位：110847 沈阳，辽宁中医药大学护理学院（王丽、孙晓婷）；辽宁省中医院消化科（王垂杰）
作者简介：王丽(1978- )，女，博士，讲师。研究方向：中医消化病基础及临床研究。Email :wltcm@126.com
更新日期/Last Update: 1900-01-01