|本期目录/Table of Contents|

[1]王晓娟.丹参多酚酸盐对大鼠骨髓间充质干细胞增殖及血管内皮生长因子表达的影响[J].环球中医药,2013,6(07):492-0.
　Wang Xiao juan..Effect of salvianolate on cell proliferation and levels of VEGF of rat bone marrow mesenchymal stem cells[J].,2013,6(07):492-0.
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丹参多酚酸盐对大鼠骨髓间充质干细胞增殖及血管内皮生长因子表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第6卷
期数:
2013年07期
页码:
492-0
栏目:
论著
出版日期:
2013-07-06

文章信息/Info

Title:
Effect of salvianolate on cell proliferation and levels of VEGF of rat bone marrow mesenchymal stem cells
作者:
王晓娟;
Author(s):
Wang Xiaojuan.
Department of pharmacy and apparatus, NO. 23 hospital of PLA, 830002 Urumqi, China
关键词:
丹参多酚酸盐; 骨髓间充质干细胞; 增殖; 血管内皮生长因子
Keywords:
Salvianolate; Mesenchymal stem cell; Proliferation; Vascular endothelial growth factor
分类号:
R2856
DOI:
-
文献标志码:
A
摘要:
目的观察中药丹参多酚酸盐对SD大鼠骨髓间充质干细胞的增殖及血管内皮生长因子(VEGF)表达的影响,以探讨其对促骨折愈合的可能机制。方法将0.5mg/ml浓度丹参多酚酸盐与大鼠骨髓间充质干细胞共培养,对照组为无药物
Abstract:
ObjectiveTo investigate the possible fracture healing mechanism of salvianolate through improving of proliferation and vascular endothelial growth factor(VEGF) levels of rat bone marrow mesenchymal stem cells(BMSC). MethodThis experiment adopted the method of cell culture in vitro. Salvianolate(05mg/ml) was coclcutured with rat BMSCs, BMSCs was cultured without Salvianolate as the control. The growth of the cells was observed under the microscope after 48 hours after culture. 1, 3, 5, 7 days later, MTT was used to detect the cell proliferation. VEGF mRNA levels of BMSCs were detected and analysed by quantitative real time polymerase chain reaction(qRTPCR) at 7 days after cell culture. ResultsThe cell growth intensity in the coculture group was significantly better than in the control group observed under microscope after 48 hours after culture. MTT assay showed that cell proliferation in experimental group were higher than in the control group after 3 days of cell culture. VEGF mRNA level in the coculture group were significantly better than the control group after 7 days of cell culture. ConclusionSalvianolate can promote rat bone marrow mesenchymal stem cell proliferation, and increase the expression of VEGF. This might be one of the possible mechanisms of promoting fracture healing.

参考文献/References:

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备注/Memo

备注/Memo:

作者单位：830002 乌鲁木齐，解放军第23医院药械科
作者简介：王晓娟(1972- )，女，本科，主管药师。研究方向：药理研究及临床应用。Email:wangxiaojuan491@126.com
更新日期/Last Update: 1900-01-01