|本期目录/Table of Contents|

[1]刘添,周建平,李绍旦,等.双虎清肝颗粒对刀豆球蛋白A所致肝损伤的防护作用机制研究[J].环球中医药,2013,6(12):881-0.
　LIU Tian,ZHOU Jian ping,LI Shao dan..Mechanism of protective effect of Shuanghu Qingan Granules on hepatic injury induced by ConA[J].,2013,6(12):881-0.
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双虎清肝颗粒对刀豆球蛋白A所致肝损伤的防护作用机制研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第6卷
期数:
2013年12期
页码:
881-0
栏目:
论著
出版日期:
2013-12-06

文章信息/Info

Title:
Mechanism of protective effect of Shuanghu Qingan Granules on hepatic injury induced by ConA
作者:
刘添;周建平;李绍旦;
Author(s):
LIU Tian;  ZHOU Jianping;  LI Shaodan.
Department of Hepatobiliary Surgery, Chinese PLA General Hospital, 100853 Beijing，China
关键词:
双虎清肝颗粒; 肝损伤; 作用机制; 一氧化氮; 巯基
Keywords:
Shuanghu Qingan Granules; Hepatic Injury; Mechanism; NO; Sulfhydryl
分类号:
R285.5
DOI:
-
文献标志码:
A
摘要:
目的从一氧化氮(nitricoxide,NO)及总蛋白巯基(totalsulfhydryl,TSH)、蛋白巯基(protein-boundsulfhydryl,PSH)的变化来探讨双虎清肝颗粒对刀豆球蛋白A(ConcanavalinA,ConA)所致肝损伤的防护作用机制。方法84只BALB/c雄性小鼠随机分成三组,正常对照组、模型对照组和双虎清肝颗粒组各28只。正常对照组给予生理盐水0.2ml尾静脉注射;模型对照组尾静脉注射ConA(15mg/kg)生理盐水溶液0.2ml;双虎清肝颗粒组每日给予双虎清肝颗粒(5mg/kg)生理盐水溶液2ml灌胃一次,连续7天后再按模型对照组处理。观察3小时、6小时、9小时及12小时时血浆谷丙转氨酶(ALT)、谷草转氨酶(AST)及NO2-含量以及肝组织TSH、PSH含量的变化情况。结果双虎清肝颗粒组各时间点血浆ALT、AST及NO2-的含量与正常对照组比较均无统计学差异(P>0.05),而与模型对照组比较均存在显著性差异(P<0.05)。双虎清肝颗粒组各时间点肝组织TSH、PSH含量与正常对照组比较均无统计学差异(P>0.05),而与模型对照组比较均有显著性差异(P<0.05)。结论双虎清肝颗粒防护肝损伤的作用机制可能是通过抑制机体NO的生物合成从而降低NO含量、阻断肝细胞TSH及PSH被持续性消耗从而提高肝组织细胞的抗氧化应激能力和解毒能力等方式实现的。
Abstract:
ObjectiveInvestigate mechanism of protective effect of Shuanghu Qingan Granules on hepatic injury induced by ConA through detecting the level of NO (nitric oxide), TSH (total sulfhydryl) and PSH (proteinbound sulfhydryl). Methods 84 male BALB/c mice were randomly divided into three groups. NS (normal saline, 02 ml) was injected via the tail vein to be regarded as the normal group. ConA (15 mg/kg) in NS (02 ml) was injected via the tail vein to be served as the model group. Shuanghu Qingan Granules (5 mg/kg) was given in NS(2 ml) by intragastric administration everyday in a week before ConA was injected via the tail vein to be served as the Shuanghu Qingan group. Plasma samples for ALT, AST and NO2 measurements were obtained at 3, 6, 9 and 12 hours after respective treatments in each group, meanwhile content of TSH and PSH of 1iver tissue homogenate. ResultsIn the Shuanghu Qingan group, level of ALT, AST and NO2 in plasma changed indistinctively at different time, vs the normal group P>005 and vs the model group P<005 respectively. Meanwhile content of TSH and PSH in 1iver tissue homogenate of the Shuanghu Qingan group also changed indistinctively at different time, vs the normal group P>005 and vs the model group P<005 respectively. ConclusionThe mechanism of protective effect of Shuanghu Qingan Granules on hepatic injury is partly due to inhibiting the biosynthesis of NO significantly and preventing the consumption of TSH and PSH markedly.

参考文献/References:

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备注/Memo

备注/Memo:
基金资助：军队中医药科研专项课题（10ZYZ241）
作者单位：100853 北京，解放军总医院肝胆外科［刘添（硕士研究生）］，中医科（李绍旦）；军事医学科学院毒物药物研究所（周建平）
作者简介：刘添（1988- ），2011级在读硕士研究生。研究方向：肝胆疾病的临床与基础研究。Email：liutian301@126.com
通讯作者：李绍旦（1976- ），博士，副主任医师。研究方向：中医及中西医结合临床与基础。Email：lsd301@126.com
更新日期/Last Update: 1900-01-01