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[1]王永,曾凯,赵红佳,等.健心颗粒对慢性心衰大鼠心室肌toll样受体4、肿瘤坏死因子-α蛋白表达的影响[J].环球中医药,2015,8(04):411-414.[doi:10.3969/j.issn.1674-1749.2015.04.007]
　WANG Yong,ZENG Kai,ZHAO Hong-jia,et al.Effects of Jianxin Granule on the TLR4 and TNF-α of ventricular muscle in rats with chronic heart failure[J].,2015,8(04):411-414.[doi:10.3969/j.issn.1674-1749.2015.04.007]
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健心颗粒对慢性心衰大鼠心室肌toll样受体4、肿瘤坏死因子-α蛋白表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年04期
页码:
411-414
栏目:
论著
出版日期:
2015-04-06

文章信息/Info

Title:
Effects of Jianxin Granule on the TLR4 and TNF-α of ventricular muscle in rats with chronic heart failure
作者:
王永; 曾凯; 赵红佳; 陈璐; 邓志兵; 陈美华
作者单位:350001 福州,福建中医药大学附属第二人民医院心内科(王永、曾凯、陈璐、邓志兵、陈美华); 福建中医药大学护理学院(赵红佳)
Author(s):
WANG Yong;  ZENG Kai;  ZHAO Hong-jia;  et al.
Cardiovascular Internal Medicine Department, The Second Affiliated Hospital of Fujian University Traditional Chinese Medicine, Fuzhou 350001, China Corresponding author: ZHAO Hong-jia, E-mail: hongjiafz@163.com
关键词:
健心颗粒;  自发性高血压大鼠;  慢性心衰;  toll样受体4;  肿瘤坏死因子-α
Keywords:
Jianxin Granule;  Spontaneously hypertensive rat;  Chronic heart failure;  Toll-like receptors 4;  Tumor necrosis factor α
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2015.04.007
文献标志码:
A
摘要:
目的 探讨健心颗粒对慢性心衰大鼠心室肌toll样受体(toll-like receptors, TLR)4、肿瘤坏死因子(tumor necrosis factor, TNF)-α蛋白表达的影响。方法 将15周龄的自发性高血压大鼠120只随机分为培哚普利组、健心颗粒低剂量组、健心颗粒中剂量组、健心颗粒高剂量组、阴性对照组,每组24只。前四组分别予培哚普利0.36 mg/(kg·d),健心颗粒1.6 g/(kg·d)、3.2 g/(kg·d)、6.1 g/(kg·d),以2 mL生理盐水溶解,阴性对照组予生理盐水2 mL,灌胃给药,每天2次。实验后每7天各组分别随机处死6只大鼠,采用免疫印迹检测上述各点心室肌组织TLR4蛋白表达,免疫组织化学显色检测上述各点心室肌组织TNF-а蛋白表达。结果 第7天,对照组、健心颗粒低、中、高剂量组间比较,大鼠心室肌TLR4、TNF-α蛋白表达无显著差异(P>0.05),但培哚普利组明显下降(P<0.05); 第14天、21天,与对照组比较,其他各组大鼠心室肌TLR4、TNF-α蛋白表达均显著下降(P>0.05),但培哚普利组较其他组下降更明显(P<0.05); 第28天,与对照组比较,其他各组大鼠心室肌TLR4、TNF-α蛋白表达均显著下降(P>0.05),但培哚普利组、健心颗粒中、高剂量组明显低于低剂量组(P<0.05)。结论 健心颗粒具有显著抗炎作用。
Abstract:
Objective To investigate the effect of the Jianxin Granule(JG)in the expression of TLR4(toll-like receptors 4)and TNF-α on ventricular muscle in rats with chronic heart failure.Methods rats(SHR-sp)120 rats, which are 15-week-old with spontaneous hypertension, were randomly divided into five groups: perindopril group 0.36 mg/(kg·d), low dose JG group 1.6 g/(kg·d), median dose JG group 3.2 g/(kg·d), high dose JG group 6.1 g/(kg·d), and negative control group(with saline 2 ml). There are 24 rats for each group. The drugs, dissolved in 2 ml saline water, were administrated by ig twice a day. During the experiment(28 days), 6 rats in each group were randomly executed every 7 days. Meanwhile the expression of TLR was determined by Western blot and pro-TNF-α by immunohistochemical staining.Result On the seventh day, there were no significant difference(P>0.05)of the expression of TLR4 and TNF-α in ventricular muscle among the control group, low, medium and high dose groups, but the perindopril group decreased obviously(P<0.05). On the fourteenth and the twenty-first day, compared with the control group, the expression of TLR4 and TNF-α in ventricular muscle groups significantly decreased(P>0.05), furthermore, the perindopril group decreased more significantly than the other groups. On the twenty-eighth day, the expression of TLR4 and TNF-α in ventricular muscle groups decreased more significantly than the control group(P>0.05), however, the perindopril group, medium and high dose groups were significantly lower than those in the low dose group(P<0.05).Conclusion Jiaxin granule has a significant anti-inflammatory effect.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:福建省自然科学基金面上项目(2011J01195); 福建省自然科学基金重大项目(2011Y4004) 作者简介:王永(1966- ),博士,主任医师,硕士生导师。研究方向:心力衰竭的防治。E-mail: wangy83706912@aliyun.com 通讯作者:赵红佳(1969- ),女,博士,教授,硕士生导师。研究方向:心力衰竭的防治。E-mail: hongjiafz@163.com
更新日期/Last Update: 2015-04-20