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[1]康楠 王凤云 陈婷 王晓鸽 朱恩林 唐旭东.参苓白术散加减方对结肠黏膜组织水通道蛋白4、水通道蛋白8表达的影响[J].环球中医药,2015,8(06):683-687.[doi:A doi:10.3969/j.issn.1674-1749.2015.06.011]
　KANG Nan,WANG Feng-yun,CHEN Ting,et al.The effects of modification of prescription of Shenling Baizhu Power on the expressions of AQP4 and AQP8 in colonic mucosa tissue[J].,2015,8(06):683-687.[doi:A doi:10.3969/j.issn.1674-1749.2015.06.011]
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参苓白术散加减方对结肠黏膜组织水通道蛋白4、水通道蛋白8表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年06期
页码:
683-687
栏目:
论著
出版日期:
2015-06-06

文章信息/Info

Title:
The effects of modification of prescription of Shenling Baizhu Power on the expressions of AQP4 and AQP8 in colonic mucosa tissue
作者:
康楠 王凤云 陈婷 王晓鸽 朱恩林 唐旭东
100029 北京中医药大学[康楠(博士研究生)、朱恩林(硕士研究生)]; 中国中医科学院西苑医院脾胃科[王凤云,陈婷(博士研究生)、王晓鸽(硕士研究生)、唐旭东]
Author(s):
KANG Nan;  WANG Feng-yun;  CHEN Ting;  et al.
Beijing University of Chinese Medicine, Beijing 100029, China
关键词:
泄泻;  脾虚证;  水通道蛋白4;  水通道蛋白8
Keywords:
Diarrhea;  Spleen deficiency;  Aquaporin 4;  Aquaporin 8
分类号:
R285.5
DOI:
A doi:10.3969/j.issn.1674-1749.2015.06.011
文献标志码:
A
摘要:
目的 观察参苓白术散加减方对脾虚泄泻模型大鼠结肠黏膜组织水通道蛋白(aquaporin,AQP)4、AQP8的表达的影响。方法 健康雄性Wistar大鼠42只随机分为正常组、模型组、高中低剂量组及西药组6组,采用水环境小平台站立法复合高乳糖饲料喂养的方法复制腹泻模型,模型成功后,灌胃给药7天,取结肠组织,通过免疫组化的方法观察结肠黏膜组织AQP4、AQP8的变化。结果 与正常组比较,模型组大鼠结肠黏膜组织AQP4、AQP8的表达明显降低; 与模型组比较,高中低剂量组及西药组均可以提高结肠黏膜组织中AQP4的表达,高低剂量组和西药组可提高AQP8的表达,中剂量组改善不明显。结论 参苓白术散加减方可提高脾虚泄泻模型大鼠结肠黏膜组织中的AQP4、AQP8的表达水平,促进水分的吸收,从而达到止泻的目的。
Abstract:
Objective To investigate the effects ofmodification of prescription of Shenling Baizhu Power on the Expressions of AQP4 and AQP8 proteins in colonic mucosa tissue. Methods Three week-old healthy male rats were randomly dividedinto six groups: control group, model group, high dose group, middle dose group, low dose group and west medicine group. The rats in the model group and treatment groups were given a high lactose diet, and were forced to stand on the multiple platforms which is full of water around to establish the type of spleen deficiency diarrhea. The medicine was given once a day for 7days. And then, AQP4 and AQP8 expression changes in the colon mucosa tissue were observed through the method of immunohistochemistry.Results The expressions of AQP4 and AQP8 in the model group significantlydecreasedcompared to that of in the control group. While compared to the model group, the expression of AQP4 in all the treatment groups were significantly increased, and the expression of AQP8 in the high and low dose groups and west medicine group were significantly increased.Conclusion The modification of prescription of Shenling Baizhu Power can antidiarrhea may via the way of increasing the expressions of AQP4 and AQP8 proteins to adjust the water absorption in colonic mucosa tissue of the rats with spleen deficiency diarrhea.

参考文献/References:

[1] 孟旸,曲瑞瑶,李梦燕,等. 实验性脾虚证结肠动力及胃肠肽和一氧化氮合酶改变[J]. 世界华人消化杂志,2000,8(8):933- 935.
[2] 张仲林,臧志和,钟玲,等. 六君子汤对脾虚证大鼠胃肠激素影响的实验研究[J]. 中成药,2010,32(4):659- 661.
[3] 赵荣华,谢鸣,李聪,等. 肝郁、脾虚和肝郁脾虚证模型大鼠的免疫功能变化[J]. 北京中医药大学学报,2013,36(12):821- 824.
[4] 杨泽民,陈蔚文. 脾虚证与物质能量代谢紊乱相关性研究进展[J]. 广州中医药大学学报,2012,29(3):332- 336.
[5] 白世敬,李峰,唐旭东,等. 功能性腹泻脾虚证动物模型制作方法[J]. 辽宁中医药大学学报,2015,17(1):86- 88.
[6] Wanjihia V W,Ohminami H,Taketani Y,et al. Induction of the hepatic stearoyl-CoA desaturase 1 gene in offspring after isocaloric administration of high fat sucrose diet during gestation[J]. J Clin Biochem Nutr,2013,53(3):150-157.
[7] Suchecki D,Tufik S. Social stability attenuates the stress in the modified multiple platform method for paradoxical sleep deprivation in the rat[J]. Physiol Behav,2000,68(3):309- 316.
[8] Fischbarg J. Fluid transport across leaky epithelia:central role of the tight junction and supporting role of aquaporins[J]. Physiol Rev,2010,90(4):1271-1290.
[9] Zeidel M L,Ambudkar S V,Smith B L,et al. Reconstitution of functional water channels in liposomes containing purified red cell CHIP28 protein[J]. Biochemistry,1992,31(33):7436-7440.
[10] Preston G M,Carroll T P,Guggino W B,et al. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein[J]. Science,1992,256(5055):385- 387.
[11] Wang K S,Ma T,Filiz F,et al. Colon water transport in transgenic mice lacking aquaporin- 4 water channels[J]. Am J Physiol Gastrointest Liver Physiol,2000,279(2):G463-G470.
[12] Yang B,Verkman A S. Water and glycerol permeabilities of aquaporins 1-5 and MIP determined quantitatively by expression of epitope-tagged constructs in Xenopus oocytes[J]. J Biol Chem,1997,272(26):16140-16146.
[13] Elkjaer M L,Nejsum L N,Gresz V,et al. Immunolocalization of aquaporin- 8 in rat kidney,gastrointestinal tract,testis,and airways[J]. Am J Physiol Renal Physiol,2001,281(6):F1047-F1057.
[14] Yang B,Song Y,Zhao D,et al. Phenotype analysis of aquaporin- 8 null mice[J]. Am J Physiol Cell Physiol,2005,288(5):C1161-C1170.
[15] Laforenza U,Cova E,Gastaldi G,et al. Aquaporin- 8 is involved in water transport in isolated superficial colonocytes from rat proximal colon[J]. J Nutr,2005,135(10):2329-2336.
[16] Hamabata T,Liu C,Takeda Y. Positive and negative regulation of water channel aquaporins in human small intestine by cholera toxin[J]. Microb Pathog,2002,32(6):273-277.
[17] Wang K S,Ma T,Filiz F,et al. Colon water transport in transgenic mice lacking aquaporin- 4 water channels[J]. Am J Physiol Gastrointest Liver Physiol,2000,279(2):G463-G470.
[18] Zahn A,Moehle C,Langmann T,et al. Aquaporin- 8 expression is reduced in ileum and induced in colon of patients with ulcerative colitis[J]. World J Gastroenterol,2007,13(11):1687-1695.
[19] 王晓玲,王俊平. 腹泻大鼠结肠水通道蛋白8表达的研究[J]. 中国药物与临床,2008,8(4):295-297.
[20] 李立胜,王俊平. 洛哌丁胺对腹泻模型大鼠结肠水通道蛋白4表达的影响[J]. 胃肠病学和肝病学杂志,2013,22(12):1225-1227.
[21] Koshimizu T A,Nakamura K,Egashira N,et al. Vasopressin V1a and V1b receptors:from molecules to physiological systems[J]. Physiol Rev,2012,92(4):1813-1864.
[22] 杨佳.血管加压素受体V_2、V_3在ACTH分泌性肿瘤上的表达及功能[D].上海:复旦大学,2013.

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备注/Memo

备注/Memo:
作者简介:康楠(1988- ),女,2012级在读博士研究生。研究方向:中医药防治消化系统疾病。E-mail:kangnan.1988@163.com 通讯作者:唐旭东(1963- ),博士,主任医师,博士生导师。研究方向:中医药防治消化系统疾病。E-mail:txdly@sina.com.cn
更新日期/Last Update: 1900-01-01