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[1]曹吴冰,高连印,车念聪,等.清毒汤对实验性重症肝损伤大鼠内毒素及D乳酸表达的影响[J].环球中医药,2015,8(11):1333-1336.[doi:10.3969/j.issn.1674-1749.2015.11.015]
　CAO Wu bing,GAO Lian yin,CHE Nian cong,et al.Effects of Qingdu decoction on endotoxin and Dlactic acid expressions in the rats with experimental severe liver injury[J].,2015,8(11):1333-1336.[doi:10.3969/j.issn.1674-1749.2015.11.015]
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清毒汤对实验性重症肝损伤大鼠内毒素及D乳酸表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年11期
页码:
1333-1336
栏目:
论著
出版日期:
2015-11-06

文章信息/Info

Title:
Effects of Qingdu decoction on endotoxin and Dlactic acid expressions in the rats with experimental severe liver injury
作者:
曹吴冰; 高连印; 车念聪; 张秋云; 杜宇琼; 付修文; 王融冰; 罗佳佳
100069北京，首都医科大学中医药学院[曹吴冰（硕士研究生）、高连印、车念聪、张秋云、杜宇琼、罗佳佳（硕士研究生）]；首都医科大学校医院内科（付修文）；首都医科大学附属北京地坛医院中西医结合科（王融冰）；中医络病研究北京市重点实验室（曹吴冰、高连印、车念聪、张秋云、杜宇琼、罗佳佳）
Author(s):
CAO Wubing;  GAO Lianyin;  CHE Niancong;  et al.
Capital Medical University School of Traditional Chinese Medicine， Beijing 100069， China
关键词:
肝损伤; 内毒素; 硫代乙酰胺; 凝血酶原活动度; D乳酸
Keywords:
Liver injury; Endotoxin; Thioacetamide; Prothrombin activity; D lactic acid
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2015.11.015
文献标志码:
A
摘要:
目的观察清毒汤对实验性重症肝损伤大鼠丙氨酸氨基转移酶（alanine aminotransferase，ALT）、内毒素、凝血酶原活动度（prothrombin activity，PTA）及D乳酸表达的影响，探讨该方治疗慢性重型肝炎内毒素血症的作用机制。方法建立TAA致实验性大鼠重症肝损伤模型，清毒汤干预后，检测大鼠ALT、内毒素、PTA及D-乳酸的表达水平。结果与正常组比较，模型组大鼠ALT、内毒素、D乳酸水平均显著升高（P＜0.01），PTA水平显著下降（P＜0.01）；与模型组比较，清毒汤低、中剂量组及乳果糖组血清ALT、内毒素、PTA及 D乳酸表达水平均有不同程度的改善（P＜0.05），清毒汤高剂量组除血清内毒素含量外各指标均明显改善（P＜0.05）；与乳果糖组比较，清毒汤中剂量组除PTA外各指标均不同程度降低（P＜0.05），低剂量组各指标均无明显差异（P＞0.05），高剂量组除PTA外各指标明显高于乳果糖组（P＜0.05）。结论清毒汤对实验性肝损伤大鼠有较好的改善肝损伤和降低内毒素表达的作用，尤其中剂量组最为明显，推测其作用机制可能与降低肠道通透性相关。
Abstract:
ObjectiveTo observe the effects of Qingdu decoction on the expressions of alanine aminotransferase (ALT), endotoxin, prothrombin activity (PTA) and Dlactic acid in rats with severe liver injury and explore the mechanism of Qingdu decoction on treating Chronic severe hepatitis complicated with endotoxemia. MethodsThe severe liver injury rat model was induced by Thioacetamide (TAA), and treated with Qingdu decoction. And then the blood level of ALT, endotoxin, PTA and Dlactic acid were measured. ResultsThe expression levels of ALT, endotoxin and Dlactic acid in the model group were significantly increased and PTA was significantly decreased compared to that of in the Normal Group (P＜001). Compared to the model group, the level of ALT, endotoxin, PTA and Dlactic acid in middle and low dose Qingdu decoction groups and Lactulose group were improved significantly (P＜005). All indexes expressions except endotoxin in the high dose Qingdu decoction group were decreased significantly (P＜005). Compared to the Lactulose group, all indexes expressions except PTA in the middle dose Qingdu decoction group were decreased significantly (P＜005). All indexes in the low dose group had no significant difference (P＞0.05), while all indexes expressions except PTA in the high dose group were significantly higher than the Lactulose group (P＜005).ConclusionQingdu decoction can improve liver injury and decrease endotoxin in rats with experimental hepatic injury complicated with endotoxemia, especially in the middle dose group. The mechanism could be related to the reduction of intestinal permeability.

参考文献/References:

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备注/Memo

备注/Memo:
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更新日期/Last Update: 1900-01-01