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[1]李磊,司宁宁,杨宾,等.祛风清热定痛方对偏头痛大鼠镇痛效应及神经递质含量的影响[J].环球中医药,2016,9(02):141-144.[doi:10.3969/j.issn.1674-1749.2016.02.004]
　LI lei,SI Ning-ning,YANG Bin,et al.Observation of Qufeng Qingre Dingtong decoction on analgesia effects and the content of NE, DA, 5-HT in migraine rats[J].,2016,9(02):141-144.[doi:10.3969/j.issn.1674-1749.2016.02.004]
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祛风清热定痛方对偏头痛大鼠镇痛效应及 神经递质含量的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年02期
页码:
141-144
栏目:
论著
出版日期:
2016-02-06

文章信息/Info

Title:
Observation of Qufeng Qingre Dingtong decoction on analgesia effects and the content of NE, DA, 5-HT in migraine rats
作者:
李磊；司宁宁；杨宾；李瑞杰；张效科
050700 河北省新乐市中医医院重症医学科(李磊、李瑞杰、杨宾); 河北省滦平县中医院内科(司宁宁); 陕西中医药大学第一临床学院(张效科)
Author(s):
LI lei;  SI Ning-ning;  YANG Bin;  et al.
Department of ICU, Xinle City Hospital of Traditional Chinese Medicine, Hebei 050700, China
关键词:
偏头痛;  祛风清热定痛方;  多巴胺;  去甲肾上腺素;  5-羟色胺
Keywords:
Migraine;  Qufeng Qingre Dingtong decoction;  Dopamine;  Norepinephrine;  5-hydroxytryptamine
分类号:
R277.7
DOI:
10.3969/j.issn.1674-1749.2016.02.004
文献标志码:
A
摘要:
目的 通过动物实验研究祛风清热定痛方的镇痛作用,并探讨其治疗偏头痛的作用机制。方法 将大鼠随机分为空白组、模型组、正天丸组、祛风清热定痛方大、小剂量组,每组8只,连续给药7天,通过热板法观察祛风清热定痛方的镇痛作用。硝酸甘油制备偏头痛大鼠模型,ELISA法检测各组大鼠脑组织中去甲肾上腺素(norepinephrine,NE)、多巴胺(dopamine,DA)及5-羟色胺(5-hydroxytryptamine,5-HT)含量,以P<0.05作为具有统计学意义的界限。 结果(1)与模型组相比,祛风清热定痛方的大剂量组自60分钟开始显著地提高了大鼠给药后的痛阈值(P<0.01); 祛风清热定痛方小剂量组、正天丸组在120分钟时提高了大鼠给药后的痛阈值(P<0.05)。(2)与模型组相比,祛风清热定痛方大剂量组、正天丸组大鼠脑组织 NE、DA 、5-HT含量均显著升高(P<0.01); 与正天丸组比较,祛风清热定痛方大剂量组有显著性差异(P<0.05)。 结论 祛风清热定痛方具有明显的镇痛的作用,其治疗偏头痛的作用机制可能是通过调节NE、DA、5-HT的释放而实现的。
Abstract:
Objective The analgesic effect of Qufeng Qingre Dingtong decoction was studied through the animal experiment research, and its mechanism of action was explored. Methods Rats were randomly divided into blank group, model group, Zhengtian Pill group, Qufeng Qingre Dingtong high and small dose groups, 8 rats in each group. Analgesic effect of Qufeng Qingre Dingtong decoction was observed by the hot plate method after continuous drug administration for 7 days. Migraine rat model was prepared by nitroglycerin. ELISA method was used to detect NE, DA and 5-HT contents in brain tissue. It was considered to be statistically significant when the value of P was less than 0.05. Results(1)Compared with the model group, Qufeng Qingre Dingtong high-dose group could significantly improve pain threshold from 60 min after the administration of drugs(P<0.01), Qufeng Qingre Dingtong low-dose group, Zhengtian pill group improved pain threshold from 120 min after administration(P<0.05).(2)Compared with the model group, NE, DA, 5-HT contents in brain tissue were very significantly increased in Qufeng Qingre Dingtong high dose group and Zhengtian Pill group(P<0.01). Compared with Zhengtian Pill group, Qufeng Qingre Dingtong high dose group had significant differences(P<0.05). Conclusion Qufeng Qingre Dingtong decoction has obvious analgesic effect and its mechanism in the treatment of migraine might achieve by regulating NE, DA and 5-HT release.

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备注/Memo

备注/Memo:
基金项目: 河北省中医药管理局中医药立项资助课题(2011141) 作者单位: 050700 河北省新乐市中医医院重症医学科(李磊、李瑞杰、杨宾); 河北省滦平县中医院内科(司宁宁); 陕西中医药大学第一临床学院(张效科)
作者简介:李磊(1981- ),本科,主治医师。研究方向:重症医学、急诊临床研究。E-mail:hebeilrj@163.com
通讯作者:李瑞杰(1979- ),硕士,主治医师。研究方向:中西医结合治疗脑血管病研究。E-mail:xinleliruijie@163.com
更新日期/Last Update: 2016-02-06