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[1]冯健男 杜守颖 白洁 陆洋 李鹏跃 武慧超.星点-效应面法优化芍药苷醇质体制备工艺[J].环球中医药,2016,9(04):409-413.[doi:10.3969/j.issn.1674-1749.2016.04.007]
　FENG Jian-nan,DU Shou-ying,BAI jie,et al.Preparationand formula optimization of Paeoniflorin ethosomes by Box-Behnken design and response surface method[J].,2016,9(04):409-413.[doi:10.3969/j.issn.1674-1749.2016.04.007]
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星点-效应面法优化芍药苷醇质体 制备工艺()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年04期
页码:
409-413
栏目:
论著
出版日期:
2016-04-06

文章信息/Info

Title:
Preparationand formula optimization of Paeoniflorin ethosomes by Box-Behnken design and response surface method
作者:
冯健男 杜守颖 白洁 陆洋 李鹏跃 武慧超
100102 北京中医药大学中药学院[冯健男(硕士研究生)、杜守颖、白洁、陆洋、李鹏跃、武慧超]
Author(s):
FENG Jian-nan;  DU Shou-ying;  BAI jie;  et al.
Department of Parmarcy, Beijing University of Chinese Medicine, Beijing 100102, China
关键词:
芍药苷;  醇质体;  Box-Behnken效应面法;  粒径;  变形性
Keywords:
Paeoniflorin;  Ethosomes;  Box-Behnken design;  Size;  Deformability
分类号:
R283
DOI:
10.3969/j.issn.1674-1749.2016.04.007
文献标志码:
A
摘要:
目的 Box-Behnken效应面法优化芍药苷醇质体的制备工艺。方法 采用逆相蒸发法制备芍药苷醇质体。以磷脂与胆固醇比例(X1)、磷脂浓度(X2)、芍药苷浓度(X3)为考察因素,包封率(Y)为评价指标,采用Box-Behnken效应面法对处方工艺进行优化。结果 最佳处方为磷脂与胆固醇比例(mg/mg)=5.0,磷脂浓度(mg/mL)=18.0 mg/mL,芍药苷浓度(mg/mL)=5.2 mg/mL。包封率为(44.27±0.27)%,标准偏差均小于10%; 粒径为(167.77±14.91)nm,多分散系数为(0.41±0.20)(n=3),变形性良好。结论 采用Box-Behnken效应面法对芍药苷醇质体处方进行优化是可行和有效的。
Abstract:
Objective To optimize the preparation technique for Paeoniflorin ethosome by Box-Behnken design and response surface method. Methods Paeoniflorin ethosomes was prepared by reverse phase evaporation.The effects of SPC- Cholesterol(X1),concentration of SPC(X2)and concentration of Paeoniflorin(X3)were observed as metrics.The encapsulation efficiency(Y)was evaluated and the formula was optimized by Box-Behnken design and response surface method. Results The optimal formula was: X1=5.0,X2=18.0,and X3=5.2. The encapsulation efficiency was(44.27±0.27)%.The standardized deviations was below 10%.The average particle size was(167.77±14.91)nm,PDI was(0.41±0.20)(n=3), deformability was satisfactory. Conclusion Using Box-Behnken design and response surface method to prepare Paeoniflorin ethosomes is feasible and effective.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 国家科技重大专项重大新药创制专项(2014ZX09301306-009)
作者简介: 冯健男(1989- ),2013级在读硕士研究生。研究方向:新剂型与新技术。E-mail:982166557@qq.com
通讯作者: 杜守颖(1960- ),女,博士,教授。研究方向:新剂型与新技术。E-mail:dushouying@263.net
更新日期/Last Update: 2016-04-06