|本期目录/Table of Contents|

[1]陈伟,田鲁,李易,等.苦瓜总皂苷对2型糖尿病肾病大鼠肾脏组织中HIF-1α和VEGF表达的作用研究[J].环球中医药,2016,9(06):659-663.[doi:10.3969/j.issn.1674-1749.2016.06.004]
　CHEN Wei,TIAN Lu,LI Yi,et al.Effects of Momordica total saponins to HIF-1α and VEGF expression in kidney tissue of type 2 diabetic nephropathy rats[J].,2016,9(06):659-663.[doi:10.3969/j.issn.1674-1749.2016.06.004]
点击复制

苦瓜总皂苷对2型糖尿病肾病大鼠肾脏组织中HIF-1α和VEGF表达的作用研究()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年06期
页码:
659-663
栏目:
论著
出版日期:
2016-06-06

文章信息/Info

Title:
Effects of Momordica total saponins to HIF-1α and VEGF expression in kidney tissue of type 2 diabetic nephropathy rats
作者:
陈伟; 田鲁; 李易; 王港; 高珊
435000 湖北省黄石市中心医院肾内科(陈伟、田鲁、李易、王港); 黄石市第二医院肾内科(高珊)
Author(s):
CHEN Wei;  TIAN Lu;  LI Yi;  et al.
Department of Nephrology, Huangshi Central Hospital of Hubei Province, Huangshi 435000, China
关键词:
苦瓜总皂苷;  缺氧诱导因子-1α;  血管内皮生长因子;  2型糖尿病;  肾功能
Keywords:
Momordica total saponins;  Hypoxia inducible factor-1;  Vascular endothelial growth factor;  Type 2 diabetes mellitus;  Renal function
分类号:
R692.39
DOI:
10.3969/j.issn.1674-1749.2016.06.004
文献标志码:
A
摘要:
目的 评价苦瓜总皂苷治疗2型糖尿病肾病大鼠的疗效。方法 雄性SD大鼠随机分为正常组、2型糖尿病肾病组(模型组)以及苦瓜总皂苷组(治疗组),每组16只,比较治疗4周后、8周后HIF-1α和VEGF表达特点。结果 与模型组相比,治疗组尿蛋白和血清肌酐明显减少,血糖明显升高(P<0.05); HE染色可见苦瓜总皂苷对糖尿病肾病带来的肾小球基底膜增厚、系膜细胞增生有一定改善; RT-PCR和Western Blot可见:与模型组相比,治疗组大鼠肾脏组织HIF-1α、VEGF蛋白和mRNA表达明显降低(P<0.05)。结论 苦瓜总皂苷可抑制HIF-1α及VEGF的表达,改善糖尿病大鼠肾功能及肾脏损害,进而延缓2型糖尿病肾病的发生发展。
Abstract:
Objective To investigate the efficacy in type 2 diabetic nephropathy rats after treated with Mcomordica total saponins. Methods Male SD rats were randomly divided into control group, diabetic nephropathy model group and Momordica total saponins treatment group. After treatment for 4 and 8 weeks, HIF-1α and VEGF expressions were detected respectively. Results Compared with the model group, there were significantly decreased in urine protein and serum creatinine, and increased in blood glucose in the treatment group(P<0.05). The pathological tissue after HE staining showed that Momordica total saponins could relieve incrassation of glomerular basement membrane, and mesangial cell proliferation. RT-PCR and Wester Blot results showed that HIF-1α and VEGF level in the treatment group were significantly lower than that in the model group(P<0.05). Conclusion Momordica total saponins could inhibit the expression of HIF-1 and VEGF, improve renal function, mitigate renal damage in diabetic rats, and delay the development of diabetic nephropathy.

参考文献/References:

[1] 韩卓笑,牛尚梅,王芸,等.肥胖与2型糖尿病肾病的研究进展[J].医学综述,2015,21(3):488-490.
[2] Bondeva T,Heinzig J,Ruhe C,et al.Advanced glycated end-products affect HIF-transcriptional activity in renal cells[J].Mol Endocrinol,2013,27(11):1918-1933.
[3] Nolan KA,Brennan EP,Scholz CC,et al. Paricalcitol protects against TGF-β1-induced fibrotic responses in hypoxia and stabilises HIF-α in renal epithelia[J].Exp Cell Res,2015,330(2):371-381.
[4] Khamaisi M,Toukan H,Axelrod JH,et al. Endothelin-converting enzyme is a plausible target gene for hypoxia-inducible factor[J].Kidney Int,2015,87(4):761-770.
[5] Thallas-Bonke V,Jha JC,Gray SP,et al. Nox-4 deletion reduces oxidative stress and injury by PKC-α-associated mechanisms in diabetic nephropathy[J].Physiol Rep,2014,2(11):e12192.
[6] 王爱民,雷闽湘,刘泽灏,等.2型糖尿病肾病危险因素分析[J],中国现代医学杂志, 2005,15(1):116-119.
[7] Seo JW,Kim YG,Lee SH,et al. Mycophenolate Mofetil Ameliorates Diabetic Nephropathy in db/db Mice[J].Biomed Res Int,2015,2015:301627.
[8] Ahad A,Ahsan H,Mujeeb M,et al. Gallic acid ameliorates renal functions by inhibiting the activation of p38 MAPK in experimentally induced type 2 diabetic rats and cultured rat proximal tubular epithelial cells[J].Chem Biol Interact,2015,240(1):292-303.
[9] Eboh C,Chowd hury TA. Management of diabetic renal disease[J].Ann Transl Med,2015,3(11):154.
[10] Kumar M1,Roe K,Nerurkar PV,et al. Impaired virus clearance,compromised immune response and increased mortality in type 2 diabetic mice infected with West Nile virus[J].PLoS One,2012,7(8):e44682.
[11] Zhang C,Xiao C,Wang P,et al. The alteration of Th1/Th2/Th17/Treg paradigm in patients with type 2 diabetes mellitus:Relationship with diabetic nephropathy[J].Hum Immunol,2014,75(4):289-296.
[12] Bai X,Geng J,Li X,et al. VEGF-A inhibition ameliorates podocyte apoptosis via repression of activating protein 1 in diabetes[J].Am J Nephrol,2014,40(6):523-534.
[13] Taiana MM,Lombardi R,Porretta-Serapiglia C,et al. Neutralization of schwann cell-secreted VEGF is protective to in vitro and in vivo experimental diabetic neuropathy[J].PLoS One,2014,9(9):e108403.
[14] 徐向进,陈频,郑全林,等.吡格列酮下调糖尿病大鼠肾脏组织缺氧诱导因子1α及血管内皮生长因子的表达[J].中华内分泌代谢杂志,2010,26(10):885-888.
[15] 董英,张慧慧.苦瓜多糖降血糖活性成分的研究[J].营养学报,2008,30(1):54-56.
[16] 马春宇,于洪宇,王慧娇,等.苦瓜总皂苷对2型糖尿病大鼠降血糖作用机制的研究[J].天津医药,2014,42(4):321-324.

相似文献/References:

备注/Memo

备注/Memo:
基金项目: 湖北省自然科学基金(2012FFA016)
作者简介: 陈伟(1981- ),硕士,主治医师。研究方向:慢性肾脏病。E-mail:506727553@qq.com
通讯作者: 高珊(1979- ),女,本科,讲师,主治医师。研究方向:肾病和肾脏透析等综合治疗。E-mail:hsyiyuantougao@163.com
更新日期/Last Update: 2016-06-06