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[1]王景霞 周恬恬 朱映黎 王成龙 吴丽 张建军.芍药苷对血虚肝郁证大鼠海马CA1区sGCβ1和5种PDE亚型蛋白表达的影响[J].环球中医药,2016,9(07):790-794.[doi:10.3969/j.issn.1674-1749.2016.07.004]
　WANG Jing-xia,ZHOU Tian-tian,ZHU Ying-li,et al.Effects of paeoniflorin on the expression of SGCβ1, different subtypes of PDE in the hippocampus CA1 Zone of rats with blood deficiency and liver depression syndrome[J].,2016,9(07):790-794.[doi:10.3969/j.issn.1674-1749.2016.07.004]
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芍药苷对血虚肝郁证大鼠海马CA1区sGCβ1和5种PDE亚型蛋白表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年07期
页码:
790-794
栏目:
论著
出版日期:
2016-07-06

文章信息/Info

Title:
Effects of paeoniflorin on the expression of SGCβ1, different subtypes of PDE in the hippocampus CA1 Zone of rats with blood deficiency and liver depression syndrome
作者:
王景霞 周恬恬 朱映黎 王成龙 吴丽 张建军
100029 北京中医药大学基础医学院中药教研室[王景霞、周恬恬(硕士研究生)、朱映黎(博士研究生)、王成龙(硕士研究生)、吴丽(硕士研究生)、张建军]
Author(s):
WANG Jing-xia;  ZHOU Tian-tian;  ZHU Ying-li;  et al.
School of Basic Medical Science, Beijing University of Chinese Medicine, Beijing 100029, China
关键词:
血虚肝郁证; 芍药苷; 环磷酸鸟苷; 磷酸二酯酶; 可溶性鸟苷酸环化酶
Keywords:
Blood deficiency and liver depression syndrome; Paeoniflorin; Cyclic guanosinc monophosphate; Phosphodiesterase; Soluble guanylyl cyclase β1
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.07.004
文献标志码:
A
摘要:
目的 研究芍药苷对血虚肝郁证大鼠海马环磷酸鸟苷(cyclic guanosinc monophosphate,cGMP)含量及CA1区cGMP合成酶即可溶性鸟苷酸环化酶(soluble guanylyl cyclaseβ1,sGCβ1)和水解酶即磷酸二酯酶(phosphodiesterase,PDE)不同亚型(PDE1A、PDE2A、PDE5A、PDE9A、PDE10A)蛋白表达的干预作用。方法 56只SD大鼠随机分为空白组、模型组、芍药苷高剂量组、芍药苷低剂量组,每组14只。采用辐射结合束缚法建立血虚肝郁证模型,空白组、模型组灌胃等量蒸馏水,给药组分别灌以40 mg/kg、 20 mg/kg的芍药苷,共造模21天。治疗结束后,采用放射免疫法测定cGMP含量; 免疫组化法检测sGCβ1、PDE1A、PDE2A、PDE5A、PDE9A、PDE10A的蛋白表达。 结果 与模型组相比,芍药苷高剂量组可降低cGMP含量(P＜0.01),并下调PDE1A、PDE2A、PDE5A、PDE9A的蛋白表达(P＜0.05),且上调PDE10A的蛋白表达(P＜0.05)。结论 芍药苷对于cGMP水解酶不同亚型存在双向调控,与抑制合成酶发挥协同作用,降低cGMP的含量,下调NO/cGMP通路,从而改善血虚肝郁证引起的大脑功能损伤。
Abstract:
Objective To observe the influence of paeoniflorin on the content of cGMP and the protein expressions of cGMP synthase namely SGCβ1 and hydrolases PDE1A, PDE2A, PDE5A, PDE9A, PDE10A in the hippocampus CA1 zone of rats with blood deficiency and liver depression syndrome. Methods The rat model was established by chronic restraint stress combined with irradiation. From the 1st day of modeling, the normal and model group was treated with same amount of distill water, the treatment group were given corresponding medicines. On the 21th day of modeling, the brain tissue and hippocampus were removed. The content of cGMP was detected by RIA. IHC was used to observe the protein expressions of sGCβ1, PDE1A, PDE2A, PDE5A, PDE9A and PDE10A. Results Compared with the model group, the content of cGMP in the dose of 40mg/kg Paeoniflorin was reduced(P＜0.01)and the protein expressions of PDE1, PDE2A, PDE5A, PDE9A were reduced(P＜0.05), and the protein expressions of PDE10A were increased significantly(P＜0.05). Conclusions Paeoniflorin could play a role of dual-directional regulation effects on the activities of different subtypes of hydrolases, which give play to synergistic effect with the inhibition of synthase, and comprehensively reduce the content of cGMP to relieve the brain damage of the blood deficiency and liver depression syndrome.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金面上项目(81173569)
作者简介: 王景霞(1976- ),女,博士,副教授,硕士生导师。研究方向:中药药性基本理论。E-mail: wjx117@sohu.com
通讯作者: 张建军(1965- ),博士,研究员,博士生导师。研究方向:基于中药基本理论的药效机理及物质基础研究。E-mail: zhangjianjun@bucm.edu.cn
更新日期/Last Update: 2016-07-06