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[1]陈伟,刘海生,魏萌,等.熊果酸对人骨肉瘤MG-63细胞增殖和凋亡的影响研究[J].环球中医药,2017,10(04):442-446.[doi:10.3969/j.issn.1674-1749.2017.04.013]
　CHEN Wei,LIU Haisheng,WEI Meng,et al.Effects of ursolic acid on the proliferation and apoptosis of human osteosarcoma MG-63 cells[J].,2017,10(04):442-446.[doi:10.3969/j.issn.1674-1749.2017.04.013]
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熊果酸对人骨肉瘤MG-63细胞增殖和凋亡的影响研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年04期
页码:
442-446
栏目:
论著
出版日期:
2017-04-06

文章信息/Info

Title:
Effects of ursolic acid on the proliferation and apoptosis of human osteosarcoma MG-63 cells
作者:
陈伟; 刘海生; 魏萌; 王丽红; 师艳艳
056001 邯郸市中心医院骨三科(陈伟、魏萌、王丽红、师艳艳); 邯郸市人民医院外一科(刘海生)
Author(s):
CHEN Wei; LIU Haisheng; WEI Meng; et al.
Third Department of Orthopedics,Handan Central Hospital,Handan 056001,China
关键词:
熊果酸;  骨肉瘤;  MG-63细胞;  增殖;  凋亡
Keywords:
Ursolic Acid;  Osteosarcoma;  MG-63 cell;  Proliferation;  Apoptosis
分类号:
R738.1
DOI:
10.3969/j.issn.1674-1749.2017.04.013
文献标志码:
A
摘要:
目的 研究熊果酸对人骨肉瘤MG-63细胞增殖和凋亡的影响及其相关机制。 方法 以不同浓度熊果酸(40、20、10 μg/mL)和顺铂(40 μg/mL)干预对数生长期人骨肉瘤MG-63细胞; 采用噻唑蓝比色法测定细胞增值抑制率,通过流式细胞术(flow cytometry,FCM)检测细胞周期和细胞凋亡状况,采用逆转录PCR(reverse transcription-polymerase chain reaction,RT-PCR)检测细胞中Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)mRNA、B细胞淋巴瘤/白血病-2(B cell lymphoma/lewkmia,bcl-2)mRNA的表达,采用免疫印迹法(western blotting,WB)检测细胞中半胱氨酸天冬氨酸蛋白酶-3(caspase-3)蛋白表达。 结果 经熊果酸干预48小时能够显著提高人骨肉瘤MG-63细胞增殖抑制率,延长细胞周期G0/G1期并缩短G2/M期,提高细胞凋亡率(apoptosis index,AI),上调Bax mRNA表达并下调bcl-2 mRNA表达、提高Bax/bcl-2比值,上调caspase-3蛋白表达,熊果酸上述作用具有一定的剂量依赖性。 结论 熊果酸具有抑制人骨肉瘤MG-63细胞增殖并促进其凋亡的作用,其机制可能与熊果酸能够阻滞细胞周期以及调节凋亡相关基因和蛋白表达有关。
Abstract:
Objective To investigate the effects of Ursolic Acid(UA)on the proliferation and apoptosis of human osteosarcoma MG-63 cells. Methods The human osteosarcoma MG-63 cells in logarithmic growth phase was treated with UA(10, 20, 40μg/mL)and Cisplatin(40μg/mL). The cellular growth inhibition rate was calculated by MTT, cell cycle and apoptosis rate were analyzed by flow cytometry, the expression of Bax mRNA and bcl-2 mRNA were detected by RT-PCR, the expression of caspase-3 protein was detected by western blotting. Results The cellular growth inhibition rate of human osteosarcoma MG-63 cell in UA treated groups were significantly increased; the G0/G1 phase of the cell cycle was prolonged and the G2/M phase was shortened, the apoptosis rate was significantly increased; the expression of Bax mRNA was significantly increased, while the expression of bcl-2 mRNA was significantly decreased, and the ratio of Bax/bcl-2 was significantly increased; the expression of caspase-3 protein was significantly increased; all of the effects above were dose-dependent with a certain. Conclusions UA can effectively inhibit human osteosarcoma MG-63 proliferation and promote it apoptosis, which perhaps related to its effects of arresting cell cycle and regulating the expression of apoptosis-related genes and proteins.

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　CHEN Wei,LIU Haisheng,WEI Meng,et al.Effects of ursolic acid on immune function of osteosarcoma rats[J].,2017,10(04):279.[doi:10.3969/j.issn.1674-1749.2017.03.006]

备注/Memo

备注/Memo:
作者简介: 陈伟(1976- ),硕士,副主任医师。研究方向:骨科疾病研究。E-mail:hdsyyzhrf@163.com
更新日期/Last Update: 2017-04-06