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[1]陈会 李新民 路岩莉 孙丹 聂坤 房艳艳 任艳艳.中药复方熄风胶囊对难治性癫痫大鼠多药耐药基因MDR1mRNA表达的影响[J].环球中医药,2017,10(07):692-696.[doi:10.3969/j.issn.1674-1749.2017.07.004]
　CHEN Hui,LI Xinmin,LU Yanli,et al.Effect of the Chinese herbal compound Xifeng capsule on expression of MDR1mRNA of intractable epilepsy rats[J].,2017,10(07):692-696.[doi:10.3969/j.issn.1674-1749.2017.07.004]
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中药复方熄风胶囊对难治性癫痫大鼠多药耐药基因MDR1mRNA表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年07期
页码:
692-696
栏目:
论著
出版日期:
2017-07-06

文章信息/Info

Title:
Effect of the Chinese herbal compound Xifeng capsule on expression of MDR1mRNA of intractable epilepsy rats
作者:
陈会 李新民 路岩莉 孙丹 聂坤 房艳艳 任艳艳
300193 天津中医药大学第一附属医院儿科(陈会、李新民、路岩莉、孙丹、聂坤 ); 天津中医药大学研究生院[房艳艳(博士研究生)]; 山东省邹城市妇幼保健计划生育服务中心(任艳艳)
Author(s):
CHEN Hui; LI Xinmin; LU Yanli; et al.
Department of Paediatrics,The First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,TianJin 300193,China
关键词:
中药复方;  难治性癫痫;  多药耐药基因;  熄风胶囊
Keywords:
Chinese herbal;  Intractable epilepsy;  MDR1mRNA;  Xifeng capsule
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.07.004
文献标志码:
A
摘要:
目的 探讨中药复方熄风胶囊对氯化锂—匹罗卡品致难治性癫痫模型大鼠脑组织MDR1mRNA表达的影响。方法 建立氯化锂—匹罗卡品癫痫大鼠模型。实验大鼠随机分为8组:正常对照组(空白组)、模型对照组(模型组)、熄风胶囊低剂量组(熄低组)、熄风胶囊中剂量组(熄中组)、熄风胶囊高剂量组(熄高组)、卡马西平治疗组(CBZ组)、熄风胶囊中剂量+卡马西平组(熄卡组)、熄风胶囊中剂量+1/2卡马西平组(熄卡低组)。熄低、中、高组分别予熄风胶囊0.33 g、0.66 g、0.99 g,浓缩剂2 mL; CBZ组予CBZ 20 mg/kg; 熄卡、熄卡低组分别予熄风胶囊0.66 g和CBZ 20 mg/kg、CBZ 10 mg/kg; 模型组和空白组分别予生理盐水2 mL。每天上午灌胃一次,共持续60天。给药结束后检测各组大鼠脑组织MDR1mRNA的表达。结果 与空白组比较,其余各组的MDR1mRNA的基因表达均上调(P＜0.05); 与模型组比较,熄中组、熄卡低组、熄卡组和CBZ组的MDR1mRNA表达均下降(P＜0.05); 与CBZ组相比,熄卡低组和熄卡组的基因表达均明显降低(P＜0.05)。结论 熄中组、熄卡低组、熄卡组、CBZ组对MDR1mRNA表达有抑制作用,且熄卡低组和熄卡组对MDR1mRNA表达的抑制作用比单用卡马西平作用更明显。
Abstract:
Objective To study the effect of Chinese herbal compound Xifeng capsule on different expression of MDR1mRNA in the brain tissue of epileptic rats induced by lithium chloride-pilocar. Methods Epileptic rat model was established by lithium chloride-pilocarpine. Rats were randomly divided into eight groups: normal control group, model control group, Xifeng capsule low-dose treatment group(Xifeng low-dose group), Xifeng capsule middle-dose treatment group(Xifeng middle-dose group), Xifeng capsule high-dose treatment group(Xifeng high-dose group), carbamazepine treatment group(CBZ group), Xifeng middle-dose + carbamazepine treatment group(xi-car group), Xifeng capsule middle-dose+1/2dose carbamazepine treatment(xi-car low group). Xifeng low, medium and high group was respectively given 0.33 g, 0.66 g and 0.99 g, thickening agent 2 mL; CBZ dose group was given CBZ 20 mg/kg; Xi-car and Xi-car low group was respectively given Xifeng capsule 0.66g thickening agentr 2mL and CBZ 20mg/kg, CBZ 10mg/kg; normal control group and model control group was respectively given 2 mL saline solution. The rats were intragastric administration once a day in the morning for 60 days. The MDR1mRNA expression of each group was detected. Result Real-time RT-PCR results showed that: comparing with normal control group, the expression of MDR1mRNA in the rest groups were up-regulation.Comparing with model group, the expression of MDR1mRNA of the CBZ group,Xifeng middle-dosegroup,Xifeng middle-dose+CBZ groupand Xifeng middle-dose+1/2 CBZ group was declined(P＜0.05). Comparing with CBZ group,the expression of MDR1mRNA of Xifeng middle-dose+CBZ groupand Xifeng middle-dose+1/2 CBZ group was declined(P＜0.05). Conclusion The CBZ group,Xifeng middle-dosegroup,Xifeng middle-dose+CBZ group and Xifeng middle-dose+1/2 CBZ group had inhibitory effect on the expression of MDR1mRNA, and Xifeng middle-dose group,the inhibitory effect of Xifeng middle-dose+1/2 CBZ group is more obvious than CBZ group.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金(81373690)
作者简介: 陈会(1985- ),女,博士,住院医师。研究方向:小儿癫痫及肺系疾病。E-mail:chenhui8586@163.com
通信作者: 李新民(1964- ),博士,教授,博士生导师。研究方向:小儿癫痫及肺系疾病。E-mail:tjtcmlxm@163.com
更新日期/Last Update: 2017-07-06