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[1]马阮昕 王晓东 江晓妹.自拟解酒护肝汤对酒精性肝炎模型大鼠的保护作用[J].环球中医药,2017,10(08):809-812.[doi:10.3969/j.issn.1674-1749.2017.08.002]
　MA Ruanxin,WANG Xiaodong,JIANG Xiaomei..Effects of Jiejiu Hugan Decoction on Alcoholic Hepatitis in Rats[J].,2017,10(08):809-812.[doi:10.3969/j.issn.1674-1749.2017.08.002]
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自拟解酒护肝汤对酒精性肝炎模型大鼠的保护作用()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年08期
页码:
809-812
栏目:
论著
出版日期:
2017-08-06

文章信息/Info

Title:
Effects of Jiejiu Hugan Decoction on Alcoholic Hepatitis in Rats
作者:
马阮昕 王晓东 江晓妹
510655 广州,中山大学附属第六医院药学部(马阮昕、王晓东); 中山大学附属第一医院药学部(江晓妹)
Author(s):
MA Ruanxin; WANG Xiaodong; JIANG Xiaomei.
The Sixth Affilited Hospital Of SUN Yatsen University,Guangzhou 510655,China
关键词:
解酒护肝汤;  酒精性肝炎;  血清;  大鼠;  肿瘤坏死因子α
Keywords:
Jiejiu Hugan Decoction;  alcoholic hepatitis;  Serum;  Rats;  TNF-α
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.08.002
文献标志码:
A
摘要:
目的 探讨自拟解酒护肝汤对酒精性肝炎模型大鼠的肝脏保护作用。方法 将SD大鼠随机分为6组,每组10只。将大鼠按体重用56%酒精给予灌胃,7 mL/kg,1天1次,持续4周。从造模后第5周起,阳性对照组灌胃给予西乐葆0.05 mg/kg,解酒护肝汤治疗组分别给予灌胃低、中、高剂量(5.0 mg/kg,15.0 mg/kg,30.0 mg/kg),持续30天。末次给药后,经腹主动脉取血,以及取肝脏组织,称重固定。随后试剂盒检测血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)、谷氨酸氨基转移酶(aspartate transaminase,AST)和丙二醛(malondialdehyde,MDA),ELISA和免疫组化法分别检测血清和肝脏肿瘤坏死因子α(tumor necrosis factor,TNF-α),HE染色观察肝脏组织形态。结果 与模型对照组比较,解酒护肝汤各剂量组的ALT、AST显著减少(P＜0.05),解酒护肝汤中、高剂量组的MDA、TNF-α显著减少(P＜0.05)。解酒护肝汤高剂量组的肝脏脏器系数显著下降(P＜0.05)。解酒护肝汤各剂量组肝脏TNF-α表达均有一定程度减少。结论 自拟解酒护肝汤对酒精性肝炎模型大鼠肝脏有一定的保护作用。
Abstract:
Objective To study the effects of Jiejiu Hugan Decoction on alcoholic hepatitis in rats. Methods SD rats were randomly divided into 6 groups(n=10). Model preparation was gastric gavage with 35% alcohol(15 ml/kg)for 4 weeks, once a day. After models were built, positive control group was given celebrex 0.05 mg/kg, and protect liver soup group was given, Jiejiu Hugan decoction low, medium and high dose(5.0 mg/kg, 15.0 mg/kg, 30.0 mg/kg)for 30 days. After the last administration, blood was collected by abdominal aortic blood, and liver tissue was weighed and fixed. The serum ALT, AST and MDA were detected. TNF-α in serum and liver was detected by ELISA and immunohistochemical method. And the HE staining was used to observe liver. Results Compared with the model group, the level of ALT, AST, MDA and TNF-α of Jiejiu Hugan decoction was significantly reduce(P＜0.05). The expressions of TNF-alpha and organ coefficients in liver were reduced in Jiejiu Hugan decoction groups and liver damage was slowed down. Conclusion Jiejiu Hugan decoction has a protect effect on alcoholic hepatitis in rats.

参考文献/References:

[1] 徐正婕,陆伦根.酒精性肝病的流行病学和自然史[J].中国处方药,2010,(1):32-33.
[2] 厉有名.酒精性肝病的流行病学特点[J].实用肝脏病杂志,2012,15(3):180-182.
[3] 陈大尧,秦文喜.饮酒对血脂影响的调查研究[J].中国预防医学杂志,2001,4(1):26-27.
[4] 关永霞,李晓梅,张永霞,等. 化滞柔肝颗粒对酒精联合脂多糖诱导的酒精性肝炎小鼠的保护作用[J].实用肝脏病杂志,2015,18(5):530-533.
[5] 陈奇.中药药理实验方法学[M].北京:人民卫生出版社,1993:837.
[6] 韩振国,王岩,李国栋.肝细胞生长因子/扩散因子和血管内皮生长因子对肿瘤血管再生的影响[J].吉林大学学报医学版,2004,30(3):376-377.
[7] 河福金,贲长恩,王德福,等.小柴胡汤对大鼠酒精性肝损伤的防护作用[J].中西医结合肝病杂志,1995,4(1):19-21.
[8] 梁扩寰,李绍白.肝脏病学[M].北京:人民卫生出版社,1995:208.
[9] Papadlm IE,Loumbourdis NS.Exposure of the frog Rana ridibunda to coppe rimpact on two biomarkers,lipid peroxidation,and glutathione[J].Bulletin Environment Contaminant Toxicology,2002,69(6):885-891.
[10] 方志红,崔剑巍,胡义扬,等.酒精性肝病的内毒素损伤机制[J].中华肝脏病杂志,2005,13(8):636-638.

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备注/Memo

备注/Memo:
作者简介: 马阮昕(1986- ),女,本科,初级药师。研究方向:药学研究。E-mail:657078890@qq.com
更新日期/Last Update: 2017-08-06