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[1]蓝伦礼 刘梦楚 邹晓红 曾元儿 曹骋 刘庆飞 李小莹 江滨.肾茶总黄酮的提取纯化工艺优化[J].环球中医药,2017,10(09):933-939.[doi:10.3969/j.issn.1674-1749.2017.09.001]
　LAN Lunli,LIU Mengchu,ZOU Xiaohong,et al.Optimization of extraction and purification technology of general flavone from Clerodendranthus spicatus(Thunb.)C.Y.Wu[J].,2017,10(09):933-939.[doi:10.3969/j.issn.1674-1749.2017.09.001]
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肾茶总黄酮的提取纯化工艺优化()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年09期
页码:
933-939
栏目:
论著
出版日期:
2017-09-06

文章信息/Info

Title:
Optimization of extraction and purification technology of general flavone from Clerodendranthus spicatus(Thunb.)C.Y.Wu
作者:
蓝伦礼 刘梦楚 邹晓红 曾元儿 曹骋 刘庆飞 李小莹 江滨
510006 广州中医药大学中药学院中药分析实验室[蓝伦礼(博士研究生)、刘梦楚、邹晓红、曾元儿、曹骋、刘庆飞、李小莹、江滨]
Author(s):
LAN Lunli; LIU Mengchu; ZOU Xiaohong;  et al.
Traditional Chinese medicine analysis laboratory,College of traditional Chinese medicine,Guangzhou university of Chinese medicine institute,Guangzhou,510006
关键词:
肾茶;  总黄酮;  Box-Behnken效应面法;  大孔吸附树脂
Keywords:
Clerodendranthus spicatus(Thunb.)C.Y.Wu;  General flavone;  Box-Behnken design response surface methodology;  Macroporous resin
分类号:
R284.2
DOI:
10.3969/j.issn.1674-1749.2017.09.001
文献标志码:
A
摘要:
目的 应用Box-Behnken效应面法优化肾茶提取工艺,运用大孔吸附树脂优选肾茶总黄酮的纯化工艺。方法 以肾茶总黄酮得率为评价指标,以乙醇体积分数,液料比和提取时间为考察因素,采用Box-Behnken效应面法优化加热回流提取工艺,并进行预测分析。通过静态和动态吸附试验考察大孔吸附树脂对肾茶总黄酮的纯化效果,并研究各因素条件对其纯化效果的影响,筛选主要影响指标的最佳工艺条件。结果 肾茶最佳提取工艺为:乙醇体积分数为52.52%,液料比(mL:g)为15:1,提取时间为90分钟。DM-130大孔吸附树脂较适合肾茶总黄酮的纯化,静态吸附生药浓度为0.1 g/mL,药液pH值为2; 动态吸附上样量为60 mL,吸附时间为50分钟,除杂水用量为3倍柱体积,洗脱剂为70%乙醇,洗脱剂用量为3倍树脂体积。结论 Box-Behnken效应面法用于优选肾茶的提取工艺是可行的,建立的数学模型和实验观察数据相符,DM-130大孔吸附树脂较适合肾茶总黄酮的纯化。
Abstract:
Objective To optimize extraction- technology of total flavonoids from Clerodendranthus spicatus(Thunb)C.Y.Wu by Box-Behnken design response surface methodology. To establish the purification technology of total flavonoids of Clerodendranthus spicatus. Methods Ethanol content, liquid-solid ratio and extraction time were used to examine the yield of total flavonoids. The Box-Behnken effect surface method was used to optimize the extraction process of heating reflux. The static and dynamic adsorption was used to observe the macroporous adsorption resin purification effect of total flavonoids of Clerodendranthus spicatus. The best purification process conditions were screened out and optimized. Results The best extraction technology:Optimum process parameters were obtained as followings: the ethanol content was 52.52%, liquid-solid ratio was 15:1, extraction duration was 90 min. DM-130 macroporous adsorption resin was suitable for purification of total flavonoids, The static adsorption concentration was 0.1 g/mL, pH was 2; Loading quantity of sample of dynamic adsorption was 60 mL, the adsorption time was 50 minutes. Water usage of edulcoration was three times as much as column volume; elution solvent was 70% ethanol, elution solvent volume was 3 three times as much as resin volume. Conclusion Box-Behnken design for optimization of extraction technology of Clerodendranthus spicatus(Thunb.)C.Y.Wu is feasible, The established mathematical model agrees well with the experimental observations. DM-130 is the best kind of macroporous resin for purification of Clerodendranthus spicatus(Thunb.)C.Y.Wu.

参考文献/References:

[1] 林艳芳,依专,赵应红,等.中国傣医药彩色图谱[M].昆明:云南民族出版社,2003:408.
[2] 陈寿元,段建红.猫须草临床应用举偶[J].四川中医,2013(11):51-52.
[3] 赵爱华,赵勤实,李蓉涛,等.肾茶的化学成分[J]. 云南植物研究,2004,(5):563-568.
[4] Yuliana ND,Khatib A,Link-Struensee AM,et al. Adenosine A1 receptor binding activity of methoxy flavonoids from Orthosiphon Planta stamineus[J].Planta Med,2009,75(2):132-136.
[5] Mishra,Shikha.Optimization of microwave-assisted extraction conditions for preparinglignan-rich extract from Saraca asoca bark using Box-Behnken design[J]. Pharm Biol,2015,(11):1-8.
[6] Xie H R,Hu L S,L I G Y.SH-SY5Y human neuroblastoma cell line:in vitro cell model of dopaminergic neurous in Parkinson's disease[J].chin Med J(Engl),2010,123(8):1086-1092.
[7] 游建军,李光,李宇赤,等. 肾茶总黄酮对帕金森病的神经保护作用[J]. 中国实验方剂学杂志,2015,(4):139-143.
[8] 张建海,冯彬彬,牛小花. 超声辅助响应面法优化巫山淫羊藿中朝藿定C和淫羊藿苷共同提取工艺研究[J]. 中国中医药信息杂志,2016,(3):85-88.

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备注/Memo

备注/Memo:
基金项目: 南药研究协同创新中心专项基金(2011)
作者简介: 蓝伦礼(1981- ),女,2013级在读博士研究生。研究方向:中药质量分析。E-mail:lanlunli@yeah.net
通信作者: 江滨(1954- ),女,本科,教授。研究方向:中药质量分析。E-mail:gzjiangbin@hotmail.com
更新日期/Last Update: 2017-09-06