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[1]王玲,吴秋枫.丹参多酚酸对2型糖尿病肾病大鼠肾小管间质激活素A表达的影响研究[J].环球中医药,2017,10(11):1212-1217.[doi:10.3969/j.issn.1674-1749.2017.11.006]
　WANG Ling,WU Qiufeng..Effects of Danshen polyphenolic acid on expression of activin A of kidney tube interstitial in type 2 diabetic nephropathy rats[J].,2017,10(11):1212-1217.[doi:10.3969/j.issn.1674-1749.2017.11.006]
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丹参多酚酸对2型糖尿病肾病大鼠肾小管间质激活素A表达的影响研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年11期
页码:
1212-1217
栏目:
论著
出版日期:
2017-11-06

文章信息/Info

Title:
Effects of Danshen polyphenolic acid on expression of activin A of kidney tube interstitial in type 2 diabetic nephropathy rats
作者:
王玲; 吴秋枫
430064 武汉科技大学附属天佑医院中医科
Author(s):
WANG Ling; WU Qiufeng.
Tianyou Hospital Affiliated,Wuhan University of Science and Technology, Wuhan 430064,China
关键词:
丹参多酚酸;  2型糖尿病;  肾病;  激活素A
Keywords:
Salvia miltiorrhiza polyphenolic acid;  Type 2 diabetes mellitus;  Nephropathy;  Activin A
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.11.006
文献标志码:
A
摘要:
目的 采用高脂饮食加链脲佐菌素诱导2型糖尿病大鼠模型,观察丹参多酚酸对其肾脏是否有保护作用及其保护机理。方法 选取清洁级雄性SD大鼠50只,适应性喂养10天后随机分为正常组、糖尿病肾病组(模型组)以及丹参多酚酸低、中和高剂量治疗组,每组10只,模型建立成功后,正常组和模型组给予生理盐水0.1 mL/(kg·d)注射,丹参多酚酸低、中和高剂量分别给予丹参多酚酸10 mL/(kg·d)、20 mL/(kg·d)和40 mL/(kg·d)注射,8周后处死大鼠,检测血糖、胰岛素、24小时尿蛋白,同时HE染色观察肝脏病理学,免疫组化、Western blot和RT-PCR检测肾脏组织中肾小管间质激活素A(activin A,Act-A)的表达特点。结果 与正常组相比,模型组血糖明显升高(P<0.05),胰岛素明显降低(P<0.05),丹参多酚酸治疗后,与模型组相比,高剂量组血糖明显降低(P<0.05),胰岛素含量明显升高(P<0.05),而中剂量组和低剂量组并没有差异; 与正常组相比,模型组肾脏肥大指数、24小时尿蛋白以及Scr明显升高(P<0.05),丹参多酚酸治疗后,含量均较模型组明显降低(P<0.05); 肾脏组织病理学可见,丹参多酚酸三个治疗组相较于模型组,炎症反应和肾间系膜增生明显减轻; 此外,与正常组相比,免疫组化、Western blot以及RT-PCR可见模型组Act-A明显升高(P<0.05),采用高剂量治疗后其表达明显降低(P<0.05),随着给药剂量的增加,呈明显剂量依赖性。结论 丹参多酚酸虽对降低2型糖尿病大鼠血糖以及提升胰岛素含量几乎没有作用,但其可明显改善肾脏24小时尿蛋白排泄,同时有效改善肾小管间质损伤,这可能与其可降低Act-A表达,进而延缓肾脏发生纤维化相关。
Abstract:
Objective To observe the effects of Danshen polyphenolic acid on the renal function of rats with type 2 diabetes mellitus induced by streptozotocin and high fat diet, meanwhile, analyze its protective mechanism. Methods Fifty male Sprague-Dawley rats were randomly divided into normal group, diabetic nephropathy group(model group)and low-dose, middle-dose and high-dose treatment group, 10 rats in each group. After the model was established successfully, the rats were given the corresponding treatment [normal group and model group was given 1 mL/(kg·d)rat body weight, Danshen polyphenolic acid low, medium and high doses were given Danshen polyphenolic acid 5 mg/kg, 10 mg/kg and 20 mg/kg], 8 weeks after the rats were sacrificed, blood glucose, insulin and 24 hours proteinuria were measured. At same time, HE staining was used to detect liver pathology, Western blot, immunohistochemistry and RT-PCR was used to detect the expression of Act-A protein in interstitial. Results Compared with the normal group, the blood glucose level in the model group was significantly increased(P<0.05), the insulin was significantly decreased(P<0.05). After treatment with Danshen polyphenolic acid, the blood glucose in the high dose group was significantly decreased(P<0.05), insulin was significantly increased(P<0.05), while there was no difference between the middle dose group and low dose. Compared with the normal group, the renal hypertrophy index, 24-hour proteinuria and Scr was significantly increased in the model group(P<0.05), and the salivaryol polyphenolic acid was significantly decreased(P<0.05). The pathological results of renal histopathology, in the three treatment groups compared with the model group, the inflammatory response and renal mesangial proliferation were significantly reduced after 8 weeks of treatment. In addition, the result of immunohistochemistry, Western blot and RT-PCR showed that compared with the normal group, the expression of Act-A in the model group was significantly higher than that in the control group(P<0.05), and Act-A was significantly decreased(P<0.05)after treatment with Danshen polyphenolic acid. Conclusion Danshen polyphenolic acid has little effect on reducing blood glucose and increasing insulin content in type 2 diabetic rats, but it can significantly improve urinary protein excretion and improve renal tubulointerstitial injury. And it can reduce Act-A expression, and thus delay the renal fibrosis associated.

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备注/Memo

备注/Memo:
基金项目: 湖北省科技厅资助项目(2015CFC187)
作者简介: 王玲(1982- ),女,硕士,主治医师。研究方向:中医基础与临床研究。E-mail:904719370@qq.com
通信作者: 吴秋枫(1966- ),女,本科,主任医师。研究方向:中医基础与临床研究。E-mail:hb70521@126.com
更新日期/Last Update: 2017-11-06