|本期目录/Table of Contents|

[1]王康,国生,钮妍,等.振腹推拿对T2DM大鼠骨骼肌AMPK/Glut4信号链的影响[J].环球中医药,2017,10(11):1224-1228.[doi:10.3969/j.issn.1674-1749.2017.11.008]
　WANG Kang,GUO Sheng,NIU Yan,et al.Effects of Zhenfu Tuina on AMPK/Glut4 signal chain of skeletal muscles in rats with T2DM[J].,2017,10(11):1224-1228.[doi:10.3969/j.issn.1674-1749.2017.11.008]
点击复制

振腹推拿对T2DM大鼠骨骼肌AMPK/Glut4信号链的影响()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年11期
页码:
1224-1228
栏目:
论著
出版日期:
2017-11-06

文章信息/Info

Title:
Effects of Zhenfu Tuina on AMPK/Glut4 signal chain of skeletal muscles in rats with T2DM
作者:
王康; 国生; 钮妍; 沈潜; 薛恬珏; 孟凤仙
100029 北京中医药大学东方医院推拿理疗科(王康、国生、沈潜); 北京中医药大学第三附属医院儿科(薛恬珏、钮妍); 北京中医药大学东方医院风湿免疫科(孟凤仙)
Author(s):
WANG Kang; GUO Sheng; NIU Yan; et al.
Department of Tuina,Dongfang Hospital of Beijing University of Chinese Medicine,Beijing 100078,China
关键词:
振腹推拿;  2型糖尿病;  腺苷酸活化蛋白激酶;  葡萄糖转运蛋白4
Keywords:
Zhenfu Tuina;  Type 2 diabetes mellitus;  Adenosine 5'-mon- ophosphate(AMP)-activated protein kinase;  Glucose transporter type 4
分类号:
R244.1
DOI:
10.3969/j.issn.1674-1749.2017.11.008
文献标志码:
A
摘要:
目的 观察振腹推拿对2型糖尿病(type 2 diabetes mellitus,T2DM)大鼠腓肠肌及腹直肌组织AMPK及Glut4基因转录及蛋白表达的影响,探讨其改善T2DM糖代谢的作用机制。方法 24只Wistar大鼠随机取出6只为空白组,其余高脂高糖饮食联合低剂量(35 mg/kg)链脲佐菌素腹腔注射建立T2DM模型,成模大鼠随机分为模型组、西药对照组及西药推拿组。空白对照组及模型组每日灌胃蒸馏水,西药对照组每日以250 mg/kg标准进行二甲双胍灌胃一次,西药推拿组在相同给药标准基础上每日加用振腹推拿治疗一次。分别在干预第0、2、4、8周测定大鼠FBG水平,干预8周后,测定腓肠肌与腹直肌AMPKα2、Glut4基因转录与蛋白表达。结果 与空白组比较,模型组FBG值在第0、2、6、8周时均显著上升,干预8周后,腹直肌AMPKα2及Glut4基因转录及蛋白表达均显著下降,腓肠肌AMPKα2基因转录及蛋白表达均显著下降,腓肠肌 Glut4蛋白表达显著下降。与模型组比较,西药组及西药推拿组FBG值在第2、6、8周时均显著下降,西药组腹直肌AMPKα2基因转录显著上升,Glut4基因转录及蛋白表达显著上升,西药推拿组腹直肌AMPKα2及Glut4基因转录及蛋白表达均显著上升,腓肠肌AMPKα2基因转录及蛋白表达均显著上升。结论 在二甲双胍常规治疗基础上,联合振腹推拿可进一步改善T2DM大鼠的FBG水平,其对糖代谢的改善机制与调控骨骼肌AMPK/Glut4信号链有关。
Abstract:
Objective To observe the influence of Zhenfu Tuina treatment on AMPK and Glut4 gene transcription and protein expression in gastrocnemius and rectus abdominis tissues in rats with type 2 diabetes mellitus(T2DM), so as to investigate the mechanism of improving glycometabolism in T2DM. Methods 6 Wistar rats were used as control group(C), 6 Wistar rats were given high-fat and high-sugar diets combined with intra-peritoneal injection with low dose(35 mg/kg)of streptozotocin to establish T2DM rat models. After successful established rat models, the rats were randomly divided into model group(M), western medication group(WM)and western medication combined with Tuina group(WT). Rats in C and M were given daily intragastric administration of distilled water while rats in WT were given metformin(250 mg/kg), once per day. On the basis of WM group, Zhenfu Tuina was added in the WT group. All rats were sacrificed after 8 weeks of intervention, and the level of FBG and gene transcription and protein expression of AMPK and Glut4 in gastrocnemius and rectus abdominis muscles was detected. Results Compared with C group, level of FBG in M group was significant increase at 2, 6 and 8 week, gene transcription and protein expression of AMPKα2 and Glut4 in rectus abdominis were significant decrease, gene transcription and protein expression of AMPKα2 in gastrocnemius was significant decrease and the protein expression of Glut4 in gastrocnemius was decrease. Compared with M group, level of FBG was significant decrease of WM group and WT group at 2, 6 and 8 week. Compard wiht M group, AMPKα2 gene transcription and protein expression of Glut4 in the rectus abdominis of WM was significant increase. Compared with M group, gene transcription and protein expression of AMPKα2 and Glut4 in rectus abdominus of WT was significant increase. Conclusion On the basis of conventional treatment of metformin, combined with Zhenfu Tuina can further improve the FBG level in T2DM rats, and the mechanism is related to the improvement of glucose metabolism and the regulation of AMPK/Glut4 signaling chain in skeletal muscle.

参考文献/References:

[1] World Health Organization[DB/OL]. Global report on diabetes,2016. http://www.who.int/diabetes/global-report/en.
[2] 汪会琴,胡如英,武海滨,等. 2型糖尿病报告发病率研究进展[J]. 浙江预防医学,2016,28(1):37-39,57.
[3] Steinberg GR, Kemp BE. AMPK in Health and Disease[J]. Physiol Rev,2009,89(3):1025-1078.
[4] Dugan LL, You YH, Ali SS, et al. AMPK dysregulation promotes diabetes-related reduction of superoxide and mitochondrial function[J]. J Clin Invest, 2013, 123(11):4888-4899.
[5] Shaw RJ, Lamia KA, Vasquez D, et al. The kinase LKB1 mediates glucose homeostasis in liver and therapeutic effects of metformin[J]. Science, 2005, 310(5754):1642-1646.
[6] 王权午,马颖桃. 腹部推拿改善2型糖尿病脂代谢异常的临床研究[J]. 临床医药文献电子杂志,2015,2(31):6373,6376.
[7] 王朝辉,韩东岳,王之虹,等. 腹部推拿结合二甲双胍对肥胖2型糖尿病患者糖脂代谢的影响[J].中国老年学杂志,2014,34(24):6874-6875.
[8] 宋柏林,朴春丽,陈曦,等. 推拿配合二甲双胍治疗肥胖2型糖尿病患者80例临床观察[J].世界中西医结合杂志,2011,6(3):206-209.
[9] 陈鹏辉,杨冰,季然,等. 振腹对2型糖尿病大鼠血清游离脂肪酸和甘油三酯的影响[J].环球中医药,2015,8(9):1070-1074.

相似文献/References:

[1]冯建华,李洁,王殿云,等.清热解毒方对2型糖尿病模型大鼠血清炎症因子TNF-α、IL-1、IL-6的干预研究[J].环球中医药,2012,5(08):573.
　FENG Jian-hua,LI Jie,WANG Dian-yun,et al.Influence of Qingre Jiedu Decoction on TNF-α,IL-1,IL-6 in type 2 diabetic rats[J].,2012,5(11):573.
[2]宿世震,项东宇,孟繁伟,等.翻白草黄酮对2型糖尿病大鼠胰岛细胞的保护作用及机制研究[J].环球中医药,2016,9(02):145.[doi:10.3969/j.issn.1674-1749.2016.02.005]
　XU Shi-zhen,XIANG Dong-yu,MENG Fan-wei,et al.Flavonoids from Potentilla discolor Bunge playing a protective and mechanical role for the type 2 diabetic rats islet cell[J].,2016,9(11):145.[doi:10.3969/j.issn.1674-1749.2016.02.005]
[3]陈伟,田鲁,李易,等.苦瓜总皂苷对2型糖尿病肾病大鼠肾脏组织中HIF-1α和VEGF表达的作用研究[J].环球中医药,2016,9(06):659.[doi:10.3969/j.issn.1674-1749.2016.06.004]
　CHEN Wei,TIAN Lu,LI Yi,et al.Effects of Momordica total saponins to HIF-1α and VEGF expression in kidney tissue of type 2 diabetic nephropathy rats[J].,2016,9(11):659.[doi:10.3969/j.issn.1674-1749.2016.06.004]
[4]陈伟强.补阳还五汤联合阿司匹林治疗2型糖尿病合并脑梗死患者50例[J].环球中医药,2016,9(09):1108.[doi:10.3969/j.issn.1674-1749.2016.09.025]
[5]刘碧原 赵静 刘进娜 谢鸣.益气养阴方对2型糖尿病大鼠肝脏糖代谢紊乱的调节作用[J].环球中医药,2016,9(10):1185.[doi:10.3969/j.issn.1674-1749.2016.10.006]
　LIU Bi-yuan,ZHAO Jing,LIU Jin-na,et al.Effects of Yiqi Yangyin formula on hepatic glucose metabolism disorder in type2 diabetes rat[J].,2016,9(11):1185.[doi:10.3969/j.issn.1674-1749.2016.10.006]
[6]王玲,吴秋枫.丹参多酚酸对2型糖尿病肾病大鼠肾小管间质激活素A表达的影响研究[J].环球中医药,2017,10(11):1212.[doi:10.3969/j.issn.1674-1749.2017.11.006]
　WANG Ling,WU Qiufeng..Effects of Danshen polyphenolic acid on expression of activin A of kidney tube interstitial in type 2 diabetic nephropathy rats[J].,2017,10(11):1212.[doi:10.3969/j.issn.1674-1749.2017.11.006]

备注/Memo

备注/Memo:
基金项目: 北京中医药大学中青年教师项目(2016-JYB-JSPY-041)
作者简介: 王康(1985- ),硕士,主治医师。研究方向:推拿改善代谢性疾病的分子机制。E-mail:wangkang_dfyy@163.com
通信作者: 孟凤仙(1955- ),女,博士,教授。研究方向:风湿免疫病、代谢病的中医临床及基础研究。E-mail:mengfx_1955@163.com
更新日期/Last Update: 2017-11-06