|本期目录/Table of Contents|

[1]张丰川,蔡玲玲,李元文,等.苦参不同制剂及成分对合轴马拉色菌标准株体外抑菌实验研究[J].环球中医药,2012,5(08):576-0.
　ZHANG feng-chuan,CAI Ling-ling,LI Yuan-wen,et al.Inhibitive effects study of different preparations and compositions of Matrine on standard strains of Malassezia sympodialis in vitro[J].,2012,5(08):576-0.
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苦参不同制剂及成分对合轴马拉色菌标准株体外抑菌实验研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第5卷
期数:
2012年08期
页码:
576-0
栏目:
论著
出版日期:
2012-08-06

文章信息/Info

Title:
Inhibitive effects study of different preparations and compositions of Matrine on standard strains of Malassezia sympodialis in vitro
作者:
张丰川; 蔡玲玲; 李元文; 赵雅静; 周志强; 赵凤珠;
Author(s):
ZHANG feng-chuan;  CAI Ling-ling;  LI Yuan-wen;  et al.
Department of Dermatology, Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China
关键词:
苦参碱;  氧化苦参碱;  合轴马拉色菌;  体外抑菌实验
Keywords:
Matrine;  Oxymatrine;  Malassezia Sympodialis;  In vitro fungistasis tests
分类号:
R285.5
DOI:
-
文献标志码:
A
摘要:
目的观察苦参的不同制剂(粉剂水提、免煎剂)及苦参碱对合轴马拉色菌标准株的抑菌作用,探讨中药对合轴马拉色菌的作用及机制。方法将实验对象分为7组:苦参粉水提组、苦参免煎颗粒组、苦参碱组、氧化苦参碱组、酮康唑组,还设了不含药物的阳性组和不含菌的阴性组。通过不同的马拉色菌培养基对苦参的不同制剂及苦参中主要生物碱进行体外抑菌研究,并同酮康唑相对照,同时测定各自的最小抑菌浓度(MIC值)、最佳抑菌浓度。结果五组的MIC值从低到高分别是:苦参碱<氧化苦参碱<酮康唑<苦参颗粒<苦参粉水提组,苦参碱组的抑菌作用比较明显,MIC值为0.3mg/ml,低于其他各组的MIC值。结论苦参的主要生物碱中苦参碱具有较好的体外抑制马拉色菌作用,氧化苦参碱与酮康唑的体外抑菌作用相当,而苦参水提剂及苦参颗粒剂体外抑菌作用较差。推测苦参碱是苦参中主要起抑菌作用的有效成分。
Abstract:
Objective Comparing Malassezia Sympodialis fungistasis of water extract of KuShen powder, KuShen granules, matrine, oxymatrine and Ketoconazole to discuss the mechanism of Chinese medicine. Methods 7 groups(KuShen powder, KuShen granules, matrine, oxymatrine and Ketoconazole)were arranged to test the inhibitive effects of different preparations and compositions of Matrine on standard strains of Malassezia sympodialis in vitro, and compare the effects with positive control group without medicine, negtive control group without Malassezia Sympodialis, and Ketoconazole group. We observed growth of Malassezia Sympodialis and detected MIC(the Minimum inhibitory concentration)and BIC(the Best inhibitory concentration)of the treatment groups. Results Every group had inhibitive effects on standard strains of Malassezia sympodialis in vitro. MIC of all the groups from low to high is matrine group, oxymatrine group, Ketoconazole group, KuShen granules group, water extract of KuShen powder group. Matrine had the best efficacy. Conclusion The main alkaloid matrine in KuShen has best inhibitory effect of Malassezia. Oxymatrine has similar function to ketoconazole. KuShen granules and Water extract of KuShen powder showed no obvious inhibitive effects. We may speculate that matrine is the main effective component of Kushen.

参考文献/References:

[1] Makimura K,Tamura Y,Kudo M,et al. Species identification and stain typing of malassezia species. stock strains and clinical isolates based on the DNA sequences of naclear ribosomal internal transcribed spacer 1 regions[J].J Med Microbiol,2000,49(1):29-35.
[2] 罗东辉,王侠生.糠秕孢子菌诱导培养角质形成细胞凋亡的研究[J].临床皮肤科杂志,2000,29(6):336-338.
[3] 高佩华.自拟白藓皮洗剂治疗脂溢性皮炎[J].中医外治杂志,2004,13(4):48.
[4] 付蓉,夏志刚,李元文,等.自制香柏波外用治疗头皮脂溢性皮炎临床观察[J].北京中医药大学学报(中医临床版),2008,15(6):8-10.
[5] 朱敏,章强强,王侠生,等.23种中草药及其14种单体抗马拉色菌体外药敏试验[J].临床皮肤科杂志,2003,32(4):193-195.
[6] 刘伟,唐金花,王亚娣,等.苦参的研究进展[J].时珍国医国药,2006,17(5):829-830.
[7] 李巍,梁鸿,尹婷,等.中药苦参主要黄酮类成分的研究[J].药学学报,2008,43(8):833-837.

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备注/Memo

备注/Memo:
基金项目:北京市教委研究生培养共建项目
作者单位：100078 北京中医药大学东方医院皮肤科(张丰川、李元文、赵雅静、周志强、赵凤珠); 北京中医药大学[蔡玲玲(博士研究生)]
作者简介：张丰川(1968- ),博士,副主任医师。研究方向:皮肤性病。E-mail:bjzfc@sina.com
更新日期/Last Update: 1900-01-01