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[1]石博,徐慧.复方苦参注射液对泪腺腺样囊性癌ACC-2细胞增殖、凋亡及Caspase-3蛋白表达的影响[J].环球中医药,2012,5(10):721-0.
　SHI Bo,XYU Hui..Effects of Compound Radix Sophorae Flavescentis injection on proliferation, apoptosis and Caspase3 expression in adenoid cystic carcinoma ACC2 cells[J].,2012,5(10):721-0.
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复方苦参注射液对泪腺腺样囊性癌ACC-2细胞增殖、凋亡及Caspase-3蛋白表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第5卷
期数:
2012年10期
页码:
721-0
栏目:
论著
出版日期:
2012-10-06

文章信息/Info

Title:
Effects of Compound Radix Sophorae Flavescentis injection on proliferation, apoptosis and Caspase3 expression in adenoid cystic carcinoma ACC2 cells
作者:
石博; 徐慧;
Author(s):
SHI Bo;  XYU Hui.
Department of Neurology，The First Hospital of Zibo,Zibo 255200,China
关键词:
复方苦参注射液;  ACC-2细胞;  增殖;  凋亡;  Caspases-3
Keywords:
Compound Radix Sophorae Flavescentis injection;  ACC2 cells; Proliferation; Apoptosis; Caspases3
分类号:
R285
DOI:
-
文献标志码:
A
摘要:
目的探讨复方苦参注射液对泪腺腺样囊性癌ACC-2细胞增殖、凋亡及Caspases-3蛋白表达的影响。方法体外培养人泪腺腺样囊性癌ACC-2细胞,应用MTT法检测细胞增殖; AnnexinV/PI双染色流式细胞仪检测细胞凋亡和细胞周期; ELISA法检测Caspases-3蛋白表达。结果复方苦参注射液对ACC-2细胞的体外增殖具有抑制作用,量效关系显著,与对照组比较有统计学差异(P<0.01),半数抑制浓度(IC50)为0.84g/ml。经流式细胞仪检测表明,复方苦参注射液能使ACC-2细胞G0-G1期逐渐增加,G2-M期和S期逐渐减少,并且随着剂量的增加,ACC-2细胞凋亡率明显增加(P<0.05或P<0.01)。复方苦参注射液能增强ACC-2细胞Caspases-3蛋白的表达(P<0.05或P<0.01),并呈剂量依赖性。结论复方苦参注射液能有效抑制人泪腺腺样囊性癌ACC-2细胞Caspases-3蛋白表达,诱导ACC-2细胞凋亡,抑制肿瘤细胞增殖。
Abstract:
ObjectiveTo investigate the effects of compound Radix Sophorae Flavescentis injection on proliferation, apoptosis and Caspase3 expression in human adenoid cystic carcinoma ACC2 cells. MethodsACC2 cells were cultured in vitro. MTT assay was used to measure the cell proliferative effect. The Annexin V/PI double staining analysis by flow cytometry was used to evaluate apoptotic rate and the cell cycle. The expression of Caspases3 protein was detected by enzymelinked immunosorbent assay (ELISA). ResultsCompound Radix Sophorae Flavescentis injection could inhibit the proliferation of ACC2 cells in vitro,and doseeffect relationship was significant(P<0.01).IC50 of ACC2 was 0.84 g/ml. The flow cytometry test indicated, compound Radix Sophorae Flavescentis injection could make ACC2 cells G0G1 phase gradually increasing, G2M period and S phase reduce gradually, and with the increase of the dose, ACC2 cell apoptosis rate increased significantly (P<0.05 or P<0.01). Compound Radix Sophorae Flavescentis injection could enhance ACC2 cells Caspases3 protein expression (P<0.05 or P<0.01), and with the dose dependent. ConclusionCompound Radix Sophorae Flavescentis injection could effectively restrain human adenoid cystic carcinoma ACC2 cells Caspases3 protein expression, induction ACC2 cells apoptosis, inhibiting tumor cell proliferation.

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备注/Memo

备注/Memo:

作者单位：255200 山东省淄博市第一医院神经内科（石博）；山东省邹城市兖矿集团东滩煤矿医院肿瘤科（徐慧）
作者简介：石博（1972- ），本科，主治医师。研究方向：神经肿瘤的临床治疗。Email：zbdyyysnsb@163.com
更新日期/Last Update: 1900-01-01