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[1]焦华琛,张蕴慧,李运伦,等.青蒿酯对房颤大鼠心肌核转录因子-κB表达的干预作用[J].环球中医药,2013,6(06):404-0.
　JIAO Hua chen,ZHANG Yun hui,LI Yun lun.The atrial fibrillation rat myocardial expression of NFκB and Artemisia annua extracts intervention role[J].,2013,6(06):404-0.
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青蒿酯对房颤大鼠心肌核转录因子-κB表达的干预作用()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第6卷
期数:
2013年06期
页码:
404-0
栏目:
论著
出版日期:
2013-06-06

文章信息/Info

Title:
The atrial fibrillation rat myocardial expression of NFκB and Artemisia annua extracts intervention role
作者:
焦华琛;张蕴慧;李运伦;
Author(s):
JIAO Huachen;  ZHANG Yunhui;  LI Yunlun
Heart disease department, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Ji Nan 250011, China
关键词:
房颤; 核转录因子-κB; 青蒿
Keywords:
Atrial fibrillation; NFκB; Artemisia annua
分类号:
R2561
DOI:
-
文献标志码:
A
摘要:
目的通过建立房颤大鼠模型,观察核转录因子-κB(nuclearfactorkappaB,NF-κB)mRNA的表达水平以及青蒿提取物对其表达的影响。方法40只大鼠按随机数字表法分为空白对照组、模型组、青蒿组、卡托普利组,每组10只。利用舌下静脉注射乙酯胆碱和氯化钙的方法建立房颤大鼠模型,使用青蒿提取物进行干预,以卡托普利作为对照药。实验前2周青蒿组以1.25g生药?kg-1灌胃,卡托普利组以3.25mg?kg-1灌胃,对照组及模型组均以等体积生理盐水灌胃。通过PCR以及Western-blot技术观察NF-κBmRNA表达以及NF-κB蛋白表达。结果与对照组相比,模型组大鼠心肌NF-κBmRNA的表达显著增加(P<0.05)。青蒿组能明显减少房颤大鼠心肌NF-κBmRNA的表达(P<0.05),卡托普利对NF-κBmRNA的表达无影响。NF-κB蛋白的表达与mRNA的表达呈一致规律。结论NF-κB与房颤的发生关系密切。房颤大鼠NF-κB的表达明显增加。青蒿提取物能够有效地减少房颤大鼠心肌NF-κB的表达,这可能是青蒿抗心律失常的机制之一。
Abstract:
bjectiveThrough the establishment of a rat model of atrial fibrillation, observed the expression of NFκB mRNA expression levels, and affect Artemisia annua extract has on it. Methods Establish a rat model of atrial fibrillation, the use of Artemisia annua extract intervention to captopril as controls. NFκB mRNA expression and protein expression were observed by PCR and Westernblot. Results Compared with the control group, model group rat cardiac NFκB mRNA expression was significantly increased (P＜005). The Artemisia annua group could significantly reduce the atrial fibrillation rat myocardial NFκB mRNA expression (P＜005). Captopril has no effect on the expression of NFκB mRNA. NFκB protein expression and mRNA expression was consistent with the law. Conclusion NFκB are closely related to the incidence of atrial fibrillation. Atrial fibrillation rat NFκB expression was significantly increased. Artemisinic extract can effectively reduce fibrillat

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：山东省中青年科学家奖励基金(2010BSA01005)
作者单位：250011 济南，山东中医药大学附属医院心病科（焦华琛、张蕴慧、李运伦）
作者简介：焦华琛(1977- )，女，博士，主治医师。研究方向：中医药治疗心律失常的基础和临床研究。Email: liyixuan0531@163com
更新日期/Last Update: 1900-01-01