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[1]徐红日,王成祥,王兰,等.RT-PCR法分析两种解表法中药对FM_1感染小鼠肺中炎性细胞因子的影响[J].环球中医药,2013,6(11):805-0.
　XU Hong ri,WANG Cheng xiang,WANG Lan,et al.Influence of the lung inflammatory cytokines in model rats infected with influenza virus FM1 by treating with two kinds of Chinese medicine compounds for reliving exterior syndromes through RTPCR method[J].,2013,6(11):805-0.
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RT-PCR法分析两种解表法中药对FM_1感染小鼠肺中炎性细胞因子的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第6卷
期数:
2013年11期
页码:
805-0
栏目:
论著
出版日期:
2013-11-06

文章信息/Info

Title:
Influence of the lung inflammatory cytokines in model rats infected with influenza virus FM1 by treating with two kinds of Chinese medicine compounds for reliving exterior syndromes through RTPCR method
作者:
徐红日;王成祥;王兰;王惠芳;张靖;殷人易;姜良铎;周平安;
Author(s):
XU Hongri; WANG Chengxiang; WANG Lan; et al.
Department of ,Emergency,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700, China
关键词:
反转录酶—聚合酶链式反应; 解表法中药; 流感病毒亚洲甲型鼠肺适应株(FM1); 小鼠; 炎性细胞因子
Keywords:
RTPCR;  Diaphoresis Chinese medicine compound for reliving exterior syndroms;  FM1;  Model rats
分类号:
R2855
DOI:
-
文献标志码:
A
摘要:
目的动态观察益气清瘟解毒合剂所含辛温解表法和辛凉解表法中药对流感病毒亚洲甲型鼠肺适应株(FM1)感染小鼠肺中炎性细胞因子表达的影响,探讨上述两种解表法抗流感免疫炎性损伤及其修复方面的作用机制。方法建立FM1感染小鼠模型,将小鼠随机分为4组,分别为正常空白组、病毒感染模型组、辛温解表法组(炙麻黄6g、羌活12g、紫苏叶10g)、辛凉解表法组(柴胡10g、金银花10g、薄荷6g),各组按病毒感染后第1、3、5、7天不同时相各分为4个小组,每小组8只小鼠。采用反转录酶-聚合酶链式反应(reversetranscriptasepolymerasechainveaction,RTPCR)法,在流感病毒感染后不同时相,动态观察辛温、辛凉解表法中药对小鼠肺中TNF-α、IL-6、IL-1、IL-10、IFN-γ5种炎性细胞因子RNA表达的影响。结果(1)辛温解表法中药在FM1感染小鼠后1
Abstract:
ObjectiveTo observe the influence of the lung inflammatory cytokines expression in model rats infected with influenza virus FM1 by treating with two kinds of Chinese medicine compounds for reliving exterior syndromes (Including relieving exterior with warmpungent herbs and coldpungent herbs in Chinese medicine theories). To discuss the inflammation damage and repairing mechanism of these two compounds.MethodsModel rats infected with influenza virus FM1 were divided into blank group, model control group, warmpungent herbs group(consists of Ephedra, Notopterygium and Purple Perilla) and coldpungent herb group(consists of Radix Bupleuri, Honeysuckle and Mentha Haplocalyx) at random. Each group was divided by the different stages of infection in 1, 3, 5 and 7 day(8 for each) Then observe the influence of TNFα, IL6, IL1, IL10 and IFNγ in model rats lungs in each groupResultsThe RNA expression in model rat lungs of TNFα in 1～5 day levels, IL1 in 3～5 day levels and IL6 in 1, 7 day levels were significantly reduced in warmpungent herb group, while the expression of IL10 in 3, 7 day levels and IFNγ in 1～3 day levels were significantly increased In coldpungent herb group, the expression of TNFα in 3～5 day levels, IL1 in 7 day level and IL6 in 1～7 day levels were significantly reduced, while the expression of IL10 in 3, 7 day levels and IFNγ in 1～3 day levels were significantly increased, but the expression of IL1 in 3 day level was significantly increased. ConclusionDiaphoresis Chinese medicine compounds for reliving exterior syndromes based on those two relieving exterior methods might be able to decrease inflammatory damages and enhance the recovery of injured by correcting inflammatory cytokine imbalance

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金（30873254）
作者单位：100700北京中医药大学东直门医院急诊科（徐红日、王兰），内科（王成祥、姜良铎）；中国人民解放军防化研究院（王惠芳、张靖）；武汉市第一医院呼吸科（殷人易）；北京中医药大学东方医院内科（周平安）。
作者简介：徐红日（1976- ），博士，副主任医师。中华中医药学会急诊分会、肺系病分会会员，世界中医药学会联合会内科分会会员。研究方向：中医药防治呼吸系统感染性疾病的临床与实验研究。Email：soohongil@sina.com
通讯作者：王成祥（1963- ），博士，主任医师，教授，博士研究生导师。中华中医药学会肺系病分会副主任委员。研究方向：中医药防治呼吸系统感染性疾病的研究。Email：wang601@vip.sina.com
更新日期/Last Update: 1900-01-01