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[1]王春林 向勇 田启东等.“拔伸松动手法”对膝骨性关节炎兔白细胞介素1β和 肿瘤坏死因子α的影响[J].环球中医药,2014,7(02):85-0.
　WANG Chun lin,XIANG Dong,TIAN Qi dong,et al.Influence on IL1β, and TNFα in the knees of rabbits with osteoarthritis by massage manipulation of stretchpullloose[J].,2014,7(02):85-0.
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“拔伸松动手法”对膝骨性关节炎兔白细胞介素1β和 肿瘤坏死因子α的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第7卷
期数:
2014年02期
页码:
85-0
栏目:
论著
出版日期:
2014-02-06

文章信息/Info

Title:
Influence on IL1β, and TNFα in the knees of rabbits with osteoarthritis by massage manipulation of stretchpullloose
作者:
王春林 向勇 田启东等
Author(s):
WANG Chunlin;  XIANG Dong;  TIAN Qidong;  et al.
The department of massage, the First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, Kunming 650021, China
关键词:
拔伸松动手法; 膝骨性关节炎; 白细胞介素1β; 肿瘤坏死因子α
Keywords:
Stretchpullloose manipulation; Knee of rabbits with osteoarthritis; IL1β; TNFα
分类号:
R2441
DOI:
-
文献标志码:
A
摘要:
目的研究拔伸松动手法对膝骨性关节炎兔膝关节液中白细胞介素1β(interleukin 1 beta, IL1β)、肿瘤坏死因子α(tumor necrosis factor alpha, TNFα)的影响。方法采用随机数字表法分为4组，即为拔伸松动手法组、注射透明质酸钠组、模型组，正常组。拔伸松动手法组手法治疗，每天一次，治疗十天后休息一天，共治疗6周；注射透明质酸钠组注射治疗，每周一次，共治疗6周；模型组正常喂养、正常组给正常喂养。结果治疗后各组关节液中IL1β浓度平均数值为模型组＞正常组＞注射透明质酸钠组＞拔伸松动手法组；TNFα浓度平均数值为模型组＞拔伸松动手法组＞注射透明质酸钠组＞正常组。用方差分析及LSDt进行两两比较分析显示，拔伸松动手法组、正常组、注射透明质酸钠组两个指标与模型组比较均为P＜005，差异有统计学意义；拔伸松动手法组、注射透明质酸钠组、正常组之间两两比较比较，两个指标均为P＞005，差异无统计学意义。结论拔伸松动手法和注射透明质酸钠，均能降低膝骨性关节炎兔关节液中IL1β、TNFα的浓度，但两种方法之间不能认为有差异。拔伸松动手法可能通过降低膝骨性关节炎兔关节液中的IL1β、TNFα浓度，从而抑制膝骨性关节炎软骨基质降解和关节软骨破坏，对软骨损伤的修复有利。
Abstract:
ObjectiveTo study the influence on interleukin 1 beta (IL1β) tumor necrosis factor alpha (TNFα) in the knees of rabbits with osteoarthritis by massage manipulation of stretchpullloose.MethodsRabbits are randomly divided into four groupsstretchpullloose manipulation group (SMG), the sodium hyaluronate injected group (SHG), the model group (MG) and the normal group (NG). Rabbits from SMG received the manipulation of stretchpullloose, once daily with a day of break every ten days, for 6 weeks. Rabbits from SHG received sodium hyaluronate injection, once weekly, for 6 weeks. Rabbits from MG and NG were given regular chow.Results After the intervention, the average level of IL1β in joint fluid showed MG> NG > SHG> SMG; and the average level of IL1β in joint fluid exhibited MG＞SMG＞SHG＞NG. Pairwise comparison among each group was drawn with the method of variance analysis and LSD  t, which. shows the two parameters observed in SMG, SHG and MG were statistically different from those in NG (P＜005)., Further, they were observed to be with no statistically significant differences among SMG, SHG and MG. ConclusionBoth stretchpullloose manipulation and sodium hyaluronate intervention serve to reduce the level of IL1β and TNFα in the knees of rabbits with osteoarthritis, while no difference can be observed between the two therapies.The mechanism of stretchpullloose manipulations effectiveness in repairing cartilage injuries in osteoarthritis, as is speculated, is by lowering the level of IL1β and TNFα in knees, which inhibit the degradation of cartilage and the destruction of bone matrix.

参考文献/References:

[1]邱贵兴．兔膝关节制动引起关节软骨退变的实验研究［J］．中华外科杂志，1987，25(3)：175177．
[2］余曙光，郭义．实验针灸学［M］．上海：上海科学技术出版社，2009：154155．
[3］Solchaga LA, Dennis JE，Goldberg VM, et a1. Hyaluronie acidbased polymers as cell carriers for tissue engineered repair of bone and cartilage［J］. J Orthop Res, 1999, 17(2): 205213.
[4］Westacott CI, Sharif M. Cytokines in ostedoarthritis: mediators or markers of joint destruction［J］. SeninArthri Rheum, 1996, 25(4): 254272.
[5］Hung GL, Galea Lauri J, Nueller GM, et a1. Suppression of Intraartieular responses to interleukinl by transfer of the Interleukin1 receptor eontagonist gene to synovium［J］. Gene Ther, 1994, 1(1): 6469.
[6］Goldring MB. The role of cytokines as inflammatory mediators in osteoarthritis: lessons from animal models［J］. Connet Tiss Res, 1999, 40(1): 111.
[7］王济维，杜宁，符诗聪，等．手法治疗实验性膝骨性关节炎研究［J］．上海第二医科大学学报．1996，16(6)：417419．
[8］唐旭昇，杜宁，张昊，等．手法治疗大鼠实验性膝骨性关节炎扫描电镜研究［J］．中医正骨，2001，13(1)：34．
[9］韩菎，熊昌源．“三联疗法”对兔膝关节骨关节炎氧自由基代谢的影响［J］．湖北中医杂志，2006，28(3)：910．
[10］王春林，赵永康，赵志勇，等．拔伸松动法治疗膝关节骨性关节炎的临床观察［J］．按摩与康复医学，2011，5(2)：2325．
[11］王春林，向勇，田启东，等．拔伸松动法治疗兔膝骨性关节炎的实验研究［J］．云南中医中药杂志，2010，31(9)：1416．

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备注/Memo

备注/Memo:
基金项目：云南省自然科学基金(2009ZC152M)
作者单位：650021昆明，云南中医学院第一附属医院推拿科(王春林、向勇、田启东、胡鸾、赵志勇、董有康、赵永康)，检验科（林云)；云南中医学院针灸推拿康复学院推拿临床教研室(邵长丽)作者简介：王春林(1968- )，本科，教授，主任医师。研究方向：脊柱推拿、膝关节骨性关节炎的中医药治疗。Email: 2478026881@qq.com
更新日期/Last Update: 1900-01-01