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[1]唐秀能 韦红棉 陆翠林等.桂产藿香蓟水提物抗炎作用及其机制研究[J].环球中医药,2014,7(06):427-0.
　TANG Xiu neng,WEI Hong mian,LU Cui lin,et al.Study on antiinflammatory effects and its mechanisms of water extract of Ageratum conyzoides L. from Guangxi[J].,2014,7(06):427-0.
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桂产藿香蓟水提物抗炎作用及其机制研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第7卷
期数:
2014年06期
页码:
427-0
栏目:
论著
出版日期:
2014-06-06

文章信息/Info

Title:
Study on antiinflammatory effects and its mechanisms of water extract of Ageratum conyzoides L. from Guangxi
作者:
唐秀能 韦红棉 陆翠林等
Author(s):
TANG Xiuneng;  WEI Hongmian;  LU Cuilin; et al.
Department of pharmacy, Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine, Nanning 530021, China
关键词:
藿香蓟; 抗炎作用; 机制
Keywords:
Ageratum conyzoides L.; Antiinflammation; Mechanism
分类号:
R2855
DOI:
-
文献标志码:
A
摘要:
目的探讨桂产藿香蓟水提物的抗炎作用及其机制。方法将60只小鼠随机分为5组：空白对照组、地塞米松组、桂产藿香蓟水提物高、中、低剂量组，采用二甲苯诱导小鼠耳廓肿胀，观察桂产藿香蓟水提物对二甲苯诱导小鼠耳廓肿胀的影响；以同样的分组方法另取60只小鼠，采用角叉菜胶致小鼠足肿胀法，观察桂产藿香蓟水提物对角叉菜胶致小鼠足肿胀的影响，并分别测定小鼠足肿胀中丙二醛、前列腺素E2和超氧化物歧化酶的活性。实验结果组间比较采用单因素方差分析，两两组间对比采用 LSD 检验。结果与空白对照组比较，桂产藿香蓟水提物高、中、低剂量组能显著抑制小鼠耳廓肿胀和足肿胀，抑制率分别为2927%、1823%、1161%和2758%、1715%、793%，差异具有统计学意义（P<001）；空白对照组前列腺素E2、丙二醛和超氧化物歧化酶含量分别为12495±1571 μg/g、2119±327 nmol/ml、16219±1252 U/ml，桂产藿香蓟水提物其高、中、低剂量组前列腺素E2、丙二醛和超氧化物歧化酶含量分别为（5212±951 μg/g、7830±1160 μg/g、8341±1334 μg/g）、（1457±251 nmol/ml、1540±234 nmol/ml、1637±318 nmol/ml）及（22276±1959 U/ml、20990±1530 U/ml、19464±1968 U/ml），与空白对照组比较，桂产藿香蓟水提物其高、中、低剂量组能显著降低小鼠炎足中炎性组织前列腺素E2、丙二醛含量，提高其超氧化物歧化酶活力，差异具有统计学意义（P<001）。结论桂产藿香蓟水提物抗炎作用显著，其机制可能与降低炎性组织中前列腺素E2的含量，提高超氧化物歧化酶活性、清除氧自由基有关。
Abstract:
ObjectiveTo explore antiinflammatory effects and its mechanisms of water extract of Ageratum conyzoides.L.from Guangxi. Methods60 mice were randomly divided into five groups:control group, dexamethasone group, water extract of Ageratum conyzoides.L.from Guangxi with high, medium and low dosage group, then induce auricle edema by dimethylbenzene to investigate the antiinflammatory effect of water extracts of Ageratum conyzoides.L.from Guangxi on auricle edema. Using the same grouping method, induce paw edema by carrageenan to investigate the antiinflammatory effect of water extracts of Ageratum conyzoides.L.from Guangxi, and measure the content of malondiadehyde(MDA), prostaglandin E2(PGE2) and the activity of superoxide dismutase(SOD)in edema paw. Difference comparison among groups was determined with single factor variance analysis. Comparison between two groups was performed with LSD test. ResultsCompared with the control group, the water extracts of Ageratum conyzoides.L.from Guangxi with high, medium and low dosage groups could remarkably inhibit the ear edema and the paw edema in mice，the inhibition ratio were 2927%, 1823%, 1161% and 2758%, 1715%, 793% respectively. The difference had statistical significance(P<001). The content of MDA, PGE2 and SOD in the edema paw in the control group were 12495±1571 μg/g, 2119±327 nmol/ml and 16219±1252 U/ml respectively. The high, medium and low dosage group were （5212±951μg/g, 7830±1160 μg/g and 8341±1334 μg/g）,（1457±251 nmol/ml, 1540±234 nmol/ml and 1637±318 nmol/ml）and（22276±1959 U/ml, 20990±1530 U/ml and 19464±1968 U/ml）respectively. Compared with control group, it could remarkably reduce MDA and PGE2 production and enhanced activity of SOD in mice inflammatory tissue, the difference had statistical significance (P<001). ConclusionThe water extracts of Ageratum conyzoides.L.from Guangxi has significantly antiinflammatory effects, and its mechanisms may relate to its ability of reducing the content of MDA and PGE2, enhancing the activity of SOD, and scavenging oxygen free radicals in mice inflammatory tissue.

参考文献/References:

［1］Adewole L, Okunade.Ageratum conyzoides L. (Asteraceae) [J]. Fitoterapia,2002,73(1):116.
［2］K.F. Chah, C.A. Eze，C.E. Emuelosi, et al.Antibacterial and wound healing properties of methanolic extracts of some Nigerian medicinal plants [J]. Journal of Ethnopharmacology, 2006, 104 (2):164167.
［3］国家中医药管理局《中华本草》编委会.中华本草[M].上海:上海科学技术出版社,1999:66786679.
［4］王馨,李晶,廖一兰,等.巴戟天水提物对老年痴呆模型大鼠的保护作用研究[J].中国药房, 2013, 24(31):29082910.
［5］唐秀能，黄仁彬，张志伟，等.月光花豆乙醇提取物的抗炎作用[J].中成药，2009,31（11）：17671769.
［6］彭红华，黄健，席雯，等. 山豆根颗粒及其饮片抗炎作用及其机制的研究[J]. 中国实验方剂学杂志, 2013,19(12)：265269.
［7］陈奇.中药药理研究方法学[M]. 3版．北京: 人民卫生出版社，2011: 409.
［8］Bitcncourt MA，Dantas GR，Lira DP, et al. Aquaous and methanolic extracts of Caulerpa mexicana suppress cell migration and ear edema induced by inflammatory agents[J].Mar Drugs，2011，9(8):13321345.
［9］何汝帮，杨安平. 复方丹参颗粒抗炎、镇痛作用的实验研究[J].中国实用医药，2009, 4(2):143144.

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备注/Memo

备注/Memo:
基金项目：广西壮族自治区卫生厅计划课题（Z2011251）
作者单位：530011 南宁，广西中医药大学附属瑞康医院药学部（唐秀能、陆翠林、梁秀群、唐云峡），手术室（韦红棉）
作者简介：唐秀能（1971），硕士，副主任药师，硕士生导师。研究方向：中药药理学及临床药学研究。Email::txnyaoshi2008@163.com
通讯作者：韦红棉（1976），本科，主管护师。研究方向：临床护理及护理药学。Email:whmtyb@163.com
更新日期/Last Update: 1900-01-01