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[1]刘勇 王洪 赵军等.针刺介入时机对缺血性中风大鼠神经功能评分和Bcl-2、Bax表达的影响[J].环球中医药,2014,7(08):581-0.
　LIU Yong,WANG Hong,ZHAO Jun,et al.Influence of acupuncture intervention time on neurological function damage of and expression of Bcl-2,Bax protein in rats after ischemic stroke[J].,2014,7(08):581-0.
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针刺介入时机对缺血性中风大鼠神经功能评分和Bcl-2、Bax表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第7卷
期数:
2014年08期
页码:
581-0
栏目:
论著
出版日期:
2014-08-06

文章信息/Info

Title:
Influence of acupuncture intervention time on neurological function damage of and expression of Bcl-2,Bax protein in rats after ischemic stroke
作者:
刘勇 王洪 赵军等
Author(s):
LIU Yong;  WANG Hong;  ZHAO Jun;  et al.
Heilongjiang University of Chinese Medicine, Harbin 150040, China
关键词:
缺血性中风; 针刺; 神经功能评分; B细胞淋巴瘤/白血病-2; Bcl-2相关X蛋白; 大鼠
Keywords:
Ischemic stroke;  Acupuncture;  Neural function score;  B-cell lymphoma/leukemia -2;  Bcl-2 associated X protein;  Rats
分类号:
R245
DOI:
-
文献标志码:
A
摘要:
目的 研究不同时间点介入针刺治疗对缺血性中风大鼠神经功能评分和对缺血脑组织中B细胞淋巴瘤/白血病-2(B cell lymphoma/lewkmia-2,Bcl-2)、Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)阳性表达的细胞数量的影响,探讨针刺治疗机制及介入时机。方法 将200只雄性SD大鼠随机分为假手术组、模型组、2小时针刺组、72小时针刺组和168小时针刺组5组,除假手术组外,均采用线栓法制备大鼠局灶性永久性脑缺血模型,在术后1、3、7、14天,4个时间点采用Garcia复合评分法评价大鼠神经功能,用酶联免疫分析法探查缺血脑组织中的Bcl-2、Ba...
Abstract:
Objective To study the impact of acupuncture treatment at different time points on neurological function score, positive expression cell number of B-cell lymphoma/leukemia-2(Bcl-2), Bcl-2 associated X protein(Bax)in ischemic brain tissue of ischemic stroke in rats, and to investigate the mechanism and intervention timing of acupuncture treatment.Methods 200 male SD rats were randomly divided into 5 groups: sham operation group, model group, 2 hours acupuncture group, 72 hours acupuncture group and 168 hours acupuncture group. Except the sham group, the rats of all the other groups were prepared into permanent focal cerebral ischemia model by suture-occluded method. 1, 3, 7, 14 days after surgery, Garcia composite score method was used to evaluate the neural function of the rats at these four time points. Enzyme league immune analysis method was used to probe the Bcl-2 and Bax protein expression in brain tissue, and the number of positive cells under a microscope was recorded for comparison.Results Neurological function score of ischemic stroke rats was significantly improved in acupuncture groups, better than the model group(P<0.001); 2 hours acupuncture group was better than that of 72 hours, 168 hours respectively(P< 0.01), and the difference was statistically significant. Bcl-2 and Bax expression in brain tissue at different time points after surgery were increased in ischemic stroke rats, one day reached a peak and then declined, and the difference was statistically significant compared with the model group(P<0.001, P<0.01). Acupuncture treatment at 2 hours after surgery can significantly increase the expression of Bcl-2 protein, inhibit the expression of Bax protein., Compared with the model group, 72 hours acupuncture group and 168 hours acupuncture group, the difference was statistically significant(P<0.001). Compared with 168 hours acupuncture group, 72 hours acupuncture group can significantly increase the expression of Bcl-2 protein, inhibit the expression of Bax protein, the difference was statistically significant(P<0.01).Conclusions Acupuncture can significantly improve the Garcia score in ischemic stroke rats, improve their motor, sensory and coordination functions; acupuncture can suppress neural cell apoptosis by regulating Bcl-2, Bax protein expression, save the function of the nerve cells in ischemic penumbra, and there is an optimal therapeutic time window of acupuncture treatment for ischemic stroke, the sooner acupuncture treatment is adopted, the better curative effect can be achieved.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:黑龙江省博士后资助项目(LBH-Z11011); 黑龙江省科技攻关项目(GC08T302)
作者单位:150040 哈尔滨,黑龙江中医药大学中西医结合博士后流动站(刘勇),研究生院[王洪(硕士研究生)、付强(硕士研究生)]; 黑龙江中医药大学附属第一医院针灸一科(刘勇、赵军)
作者简介:刘勇(1975-),女,博士,副主任医师,在站博士后。研究方向:针刺治疗缺血性中风的实验研究,针刺治疗延髓麻痹。E-mail: 24680190@qq.com
更新日期/Last Update: 1900-01-01