|本期目录/Table of Contents|

[1]邢佳,王嘉麟,贺立娟,等.泻肝安神法对睡眠剥夺大鼠氨基酸类神经递质影响的研究[J].环球中医药,2015,8(03):324-328.[doi:10.3969/j.issn.1674-1749.2015.03.015]
　XING Jia,WANG Jia-lin,HE Li-juan,et al.Study on the method of Xiegan Anshen's effects on amino acid neurotransmitters of rats after sleep deprivation[J].,2015,8(03):324-328.[doi:10.3969/j.issn.1674-1749.2015.03.015]
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泻肝安神法对睡眠剥夺大鼠氨基酸类神经递质影响的研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年03期
页码:
324-328
栏目:
论著
出版日期:
2015-03-06

文章信息/Info

Title:
Study on the method of Xiegan Anshen's effects on amino acid neurotransmitters of rats after sleep deprivation
作者:
邢佳; 王嘉麟; 贺立娟; 王椿野
100078 北京中医药大学东方医院脑病二科
Author(s):
XING Jia;  WANG Jia-lin;  HE Li-juan;  et al.
NO.2 Department of Encephalopathy, Dongfang Hospital of Beijing University of Chinese Medicine, Beijing 100078, China Corresponding author:XING Jia, E-mail:dfyyxingjia@163.com
关键词:
γ-氨基丁酸;  泻肝安神法;  水平台法;  睡眠剥夺大鼠;  氨基酸类神经递质
Keywords:
GABA;  Xiegan Anshen Decoction;  Water platform;  Sleep Deprivation Rats;  amino acid neurotransmitters
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2015.03.015
文献标志码:
A
摘要:
目的 探讨泻肝安神法对睡眠剥夺大鼠脑干、海马、下丘脑脑区氨基酸类神经递质含量的干预效应。方法 将60只SD大鼠随机分为4组,分别为正常对照组、失眠模型组、中药组、西药组,每组各15只,除正常对照组外的其余三组大鼠一律采用水平台法反复剥夺睡眠7次,每次持续24小时。造模结束后,中药组大鼠灌胃给药泻肝安神汤,西药组灌胃给药地西泮溶解液,失眠模型组灌胃给予纯净水,干预7天后取材,采用高效液相色谱法检测各组大鼠脑干、海马、下丘脑脑区6种氨基酸类神经递质的含量。结果 经方差分析发现大鼠剥夺睡眠后脑干氨基酸类神经递质门冬氨酸下降,γ-氨基丁酸升高,下丘脑γ-氨基丁酸下降(P<0.05),而在海马和下丘脑区域各氨基酸类神经递质含量变化不显著(P>0.05)。采取泻肝安神法治疗后大鼠脑干门冬氨酸含量显著升高,下丘脑γ-氨基丁酸含量升高(P<0.05); 而采用地西泮片治疗后大鼠脑干区门冬氨酸升高,下丘脑门冬氨酸、γ-氨基丁酸、甘氨酸、牛磺酸升高(P<0.05)。结论 泻肝安神法可调节睡眠剥夺大鼠的氨基酸类神经递质,在下丘脑的作用机制类似于地西泮片,但对于氨基酸类神经递质的影响影响靶点要少于西药。
Abstract:
ObjectiveTo investigate the Xiegan Anshen intervention of contents of amino acid neurotransmitters in brainstem,hypothalamus and hippocampus of rats after sleep deprivation. Methods 60 SD rats were randomly divided into 4 groups(each having 15 rats): normal control group, insomnia model group, Chinese medicine group and Western medicine group. Apart from the normal control group, other three groups of rats are repeatedly deprived of sleep for 7 times by using level station method, each lasting 24 hours. After modeling, rats of Chinese medicine group, western medicine group and insomnia model group were intragastrically administered with Xiegan Anshen Decoction, diazepam solution and pure water respectively. Rats then were killed 7 days after the intervention. HPLC method was used to detect 6 kinds of amino acid neurotransmitters in brainstem, hippocampus and hypothalamus regions. Results After sleep deprivation, the content of Aspartate in brainstem decreased significantly; the content of GABA in brainstem increased, and the content of GABA in hypothalamus decreased significantly. There were no significant changes in hippocampus. After treatment by Xiegan Anshen decoction, the content of Aspartate in brainstem was significantly increased, and the content of GABA in hypothalamus increased too. After treatment by Diazepam Tablets, the content of Aspartate in brainstem was significantly increased, and the contents of Aspartate, GABA, Glycine and Taurine in hypothalamus increased. Conclusion Xiegan Anshen decoction can regulate the content of amino acid neurotransmitters of sleep deprivation rats, and the mechanism of action of Xiegan Anshen Decoction in hypothalamus is similar to Diazepam Tablets, but its effects on the targets are less effective than those of Diazepam Tablets.

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备注/Memo

备注/Memo:
基金项目:2010年北京中医药大学自主课题(JYB22-JS046); 2012年教育部博士点新教师类基金(20120013120014); 2011年北京市科技计划(Z111107056811040); 北京中医药大学创新团队发展计划(2011-CXTD-23); 2013年北京中医药大学科研基金青年教师专项 作者简介:邢佳(1983- ),女,硕士,主治医师。研究方向:中医脑病。E-mail:dfyyxingjia@163.com
更新日期/Last Update: 2015-03-20