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[1]王建伟,金香兰,刘雪梅,等.益肾化浊法对慢性脑缺血血管性痴呆大鼠脑组织海马HIF-1α和VEGF表达的影响[J].环球中医药,2015,8(04):403-406.[doi:10.3969/j.issn.1674-1749.2015.04.005]
　WANG Jian-wei,JIN Xiang-lan,LIU Xue-mei,et al.Influence of Yishen Huazhuo Therapy on expression of HIF-1α and VEGF in hippocampus of rats with chronic ischemic vascular dementia[J].,2015,8(04):403-406.[doi:10.3969/j.issn.1674-1749.2015.04.005]
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益肾化浊法对慢性脑缺血血管性痴呆大鼠脑组织海马HIF-1α和VEGF表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年04期
页码:
403-406
栏目:
论著
出版日期:
2015-04-06

文章信息/Info

Title:
Influence of Yishen Huazhuo Therapy on expression of HIF-1α and VEGF in hippocampus of rats with chronic ischemic vascular dementia
作者:
王建伟; 金香兰; 刘雪梅; 梁晓; 闫妍; 郑宏; 张允岭
作者单位:100029 北京中医药大学第二临床医学院[王建伟(硕士研究生)、梁晓(博士研究生)]; 北京中医药大学东方医院脑病二科(金香兰); 北京中医药大学东方医院实验中心(刘雪梅、闫妍、郑宏、张允岭)
Author(s):
WANG Jian-wei;  JIN Xiang-lan;  LIU Xue-mei;  et al.
The second clinical medical college, Beijing University of Chinese Medicine, Beijing 100029, China Corresponding author: JIN Xiang-lan, Emal: jxlan2001@126.com
关键词:
血管性痴呆;  益肾化浊法;  缺氧诱导因子1α;  血管内皮生长因子
Keywords:
Vascular dementia;  Yishen Huazhuo Therapy;  hypoxia-inducible factor-1α;  vascular endothelial growth factor
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2015.04.005
文献标志码:
A
摘要:
目的 观察益肾化浊法对慢性脑缺血血管性痴呆大鼠脑组织海马低氧诱导因子-1α(hypoxia-inducible factor-1,HIF-1α)和血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响。方法 采用双侧颈总动脉永久性阻断法建立血管性痴呆大鼠模型,造模后7天随机分为假手术组,模型组和聪圣颗粒低、中、高剂量组和尼麦角林片组,给药治疗60天后,采用Western-blot及Real Time-PCR方法检测大鼠海马HIF-1α、VEGF表达水平。结果 与假手术组比较,模型组大鼠HIF-1α和VEGF的蛋白表达量和mRNA的表达量均升高(P<0.05); 与模型组比较,聪圣颗粒中、高剂量组可升高模型组HIF-1α及VEGF的蛋白和mRNA的表达(P<0.05,P<0.01)。结论 益肾化浊法对慢性脑缺血血管性痴呆大鼠作用机制可能与调控海马区HIF-1α和VEGF的表达有关。
Abstract:
Objective To observe the influence of Yishen Huazhuo Therapy on expression of hypoxia-inducible factor-1(HIF-1α)and vascular endothelial growth factor(VEGF)in hippocampus of rats with chronic ischemic vascular dementia. Methods Vascular dementia rat models were established by using bilateral carotid artery block method. 7 days after animal model establishment, rats were randomly divided into sham-operated group, model group, high, middle and low dosage of Shengcong Particle groups and nicergoline tablets group. After 60 days of treatment, the expression level of HIF-1α and VEGF in hippocampus of rats was tested by using Western blot and Real time PCR. Results Compared with sham-operated group, the protein and mRNA expression of HIF-1α and VEGF in hippocampus of rats in model group was increased significantly(P<0.05). Compared with model group, the protein and mRNA expression of HIF-1α and VEGF in hippocampus of rats in high and middle dosage of Shengcong Particle group was increased significantly(P<0.05, P<0.01). Conclusion Yishen Huazhuo therapy could improve the status of rats with chronic ischemic vascular dementia and the mechanism might be related with the regulation on expression of HIF-1α and VEGF in hippocampus of rats.

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备注/Memo

备注/Memo:
基金项目:高等学校博士学科点专项科研基金(20110013120014); 国家自然科学基金(81302959). 作者简介:王建伟(1987- ),2012级在读硕士研究生。研究方向:中医药防治脑血管病及痴呆。E-mail:wangjw1987@126.com 通讯作者:金香兰(1976- ),女,博士,副主任医师。研究方向:中医药防治脑血管病及痴呆,E-mail:jxlan2001@126.com
更新日期/Last Update: 2015-04-20