|本期目录/Table of Contents|

[1]彭园园,张莉,刘通等.电针“委中”穴对大鼠腰多裂肌损伤后的再生促进 作用及对IGF1表达的影响[J].环球中医药,2015,8(05):513-517.[doi:10.3969/j.issn.16741749.2015.05.001]
　PENG Yuan yuan,ZHANG Li,LIU Tong,et al.Effect of electroacupuncture at BL40 on morphology repairment and IGF1 expression in rats with multifidus muscle injury[J].,2015,8(05):513-517.[doi:10.3969/j.issn.16741749.2015.05.001]
点击复制

电针“委中”穴对大鼠腰多裂肌损伤后的再生促进 作用及对IGF1表达的影响()
     
分享到：

《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年05期
页码:
513-517
栏目:
论著
出版日期:
2015-05-06

文章信息/Info

Title:
Effect of electroacupuncture at BL40 on morphology repairment and IGF1 expression in rats with multifidus muscle injury
作者:
彭园园; 张莉; 刘通等
100029，北京中医药大学针灸推拿学院\[彭园园(硕士研究生)、张莉、刘通（博士研究生）、陈玉佩（硕士研究生）\]，基础医学院（葛东宇），科研实验中心（毛颖秋）
Author(s):
PENG Yuanyuan;  ZHANG Li;  LIU Tong;  et al.
School of Acupunturemoxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China Corresponding author: ZHANG Li, Email: zhangli1572@sina.com
关键词:
电针; 多裂肌损伤; 布比卡因; 委中; 肾俞; 胰岛素样生长因子1
Keywords:
Electroacupuncture; Multifidus muscle injury; Bupivacaine; BL40;  BL23; IGF1
分类号:
R245
DOI:
10.3969/j.issn.16741749.2015.05.001
文献标志码:
A
摘要:
目的观察电针委中穴对腰部多裂肌损伤后的干预作用及对胰岛素样生长因子1（insulin like growth factor 1，IGF1）表达的影响，探求委中穴对大鼠腰肌损伤后再生修复的可能机制。方法雄性SD大鼠120只，随机分为空白组、模型对照组、模型组、电针委中组、电针肾俞组，共5组，观察3个时间点（4天、7天、14天）的变化，每个时间点8个样本。 将0.5%布比卡因盐酸盐溶液按每点100 μL注射于模型组和电针组大鼠L4、L5水平的多裂肌上。模型对照组采用同样方法注射生理盐水，空白组不做处理。造模后进行电针双侧委中穴或肾俞穴分别治疗4天、7天、14天，HE染色观察肌细胞形态学的改变，免疫组化方法检测肌细胞IGF1的表达。结果造模前后多裂肌形态学改变显著，损伤后第14天仍未完全恢复。电针委中组与电针肾俞组从形态学上优于模型组。第4、7天模型组多裂肌IGF1的表达显著高于空白组（P＜001）；第4天电针委中组表达显著高于模型组（P＜001），电针委中组表达高于电针肾俞组（P＜005），电针肾俞组表达高于模型组（P＜005），而在第14天，电针肾俞组表达显著高于模型组与电针委中组（P＜001）。结论电针委中穴和电针肾俞穴均能够促进大鼠腰多裂肌损伤后的再生，电针委中穴在肌肉损伤的早期效果显著。
Abstract:
ObjectiveTo observe the effect of electroacupuncture on multifidus musle injury by morphological observation and insulin like growth factor 1(IGF1) expression of rats with bupivacaine induced multifidus muscle toxicity, and discuss the possible mechanism of muscle tissue regeneration. Methods120 male Sprague Dawley rats were randomly divided into blank group (BG), model control group(CG), model group (MG), Weizhong (BL40) electroacupuncture group (WG) and Shenshu(BL23) electroacupuncture group (SG), each group then was divided evenly into three time points (day4, day7 and day14) and each time point has 8 samples. Multifidus muscle (L4 and L5 sections) toxicity was established by injecting 0.5% bupivacaine hydrochloride, and normal saline injection as control (CG). The WG and LG accepted electroacupuncture for 4d, 7d or 14d after modeling. The multifidus muscle of each rodent was stained with HE to observe pathological changes, and immunehistochemical staining was used to observe IGF1 expression. ResultsBupivacaine caused a process of degenerationregeneration of the muscle fibers and did not normalize at day 14. WG and SG were recovered faster than MG. The expression of IGF1 at day 4 ranked as MG higher than BG (P<0.01), WG higher than MG and SG (P<0.01, P<0.05), and SG higher than MG (P<0.05), while ranked as SG higher than MG and WG (P<0.01) at day14. Conclusion Electroacupuncture at both BL40 and BL23 could promote the regeneration of tissue after multifidus muscle injury, and it seems better therapy effect when electroacupuncture at BL40.

参考文献/References:

[1]Huang Q, Zhang Y, Li D, et al. The Evaluation of Chronic Low Back Pain by Determining the Ratio of the Lumbar Multifidus Muscle Crossｋsectional Areas of the Unaffected and Affected Sides[J]. Journal of Physical Therapy Science, 2014, 26(10): 1613.
[2]吴巍巍,胡志军,范顺武,等. 慢性腰痛对脊旁多裂肌萎缩影响的临床研究[J]. 中国骨伤,2014, 27(3): 207212.
[3]Silfies S P, Spuillante D, Maurer P, et al. Trunk muscle recruitment Pattems in specific chronic low hack pain populatios[J]. clin Biomech, 2005, 20(5): 465473.
[4]Engert J C, Berglund E B, Rosenthal N. Proliferation precedes differentiation in IGFIstimulated myogenesis[J]. J Cell Biol, 1996, 135(2):431440.
[5]陈欢,彭博,李富运,等. 电针对兔腰肌急性钝挫伤后组织修复与碱性成纤维细胞生长因子/细胞外信号调节激酶信号通路的影响[J]. 中国康复理论与实践,2014,20(3):215220.
[6]Toru Taguchi, Ulrich Hoheisel, Siegfried Mense. Dorsal horn neurons having input from low back structures in rats[J]. Pain, 2008, 138(1):119129.
[7]李忠仁. 实验针灸学[M]. 中国中医药出版社,2003:316.
[8]Vos T, Flaxman A D, Naghavi M, et al. Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 19902010: a systematic analysis for the Global Burden of Disease Study 2010[J]. The Lancet, 2013, 380(9859): 21632196.
[9]Haldeman S, KopanskyGiles D, Hurwitz EL, et al. Advancements in the management of spine disorders[J]. Best Pract Res Clin Rheumatol, 2012, 26(2): 263280.
[10]Zanou N, Gailly P. Skeletal muscle hypertrophy and regeneration: interplay between the myogenic regulatory factors (MRFs) and insulinlike growth factors (IGFs) pathways[J]. Cellular and Molecular Life Sciences, 2013, 70(21): 41174130.
[11]王研,高维滨. 运用T字型取穴法电针治疗下肢肌肉损伤的临床观察[J]. 针灸临床杂志,2012,28(9):2325.
[12]邢崇慧,李冬梅,张蔚. 电针配合超声波治疗运动员肌肉损伤108例[J]. 上海针灸杂志,2004,23(5):1819.
[13]徐前威,王荣国,王云亭,等. 电针对兔钝器伤后骨骼肌中转化生长因子β1和波形蛋白表达的影响[J]. 中国康复医学杂志,2014,29(5):421426.
[14]陈欢,张莉,崔强,等. 电针阿是穴对骨骼肌损伤大鼠肌肉再生及碱性成纤维细胞生长因子表达的影响[J]. 中国康复理论与实践,2013,19(4):334340.
[15]马忆南,杨华元,冯麟,等. 针刺诱导胶原信号变化对骨骼肌损伤的修复作用[J].中国组织工程研究,2012,16(42):78887892.
[16]贾红玲,张学伟,张永臣. 数据挖掘技术在针灸治疗腰痛古代文献中的应用[J]. 针灸临床杂志,2013,29(11):4045.
[17]杨辉.腰痛的中医辩证及针灸治疗[J].中医临床研究,2011,3(20):7071.
[18]郭桂霞,张阿宏. 综合护理雷火灸治疗寒湿型腰肌劳损[J]. 实用中医内科杂志,2014,28(7):160162.
[19]卜彦青,杜广中. 委中穴现代疾病谱[J]. 中国中医基础医学杂志,2011,17(10):11371139.
[20]徐青燕,杨金生,杨莉,等.委中穴区刮痧对本经同侧经脉线上皮肤微循环血流灌注量的影响[J].针刺研究,2013,38(1):5256.
[21]王苓苓,张维波,谢衡辉,等.使用血流成像技术对“腰背委中求”经典理论的验证[J].针刺研究, 2007,3(4):247251.
[22]金末淑,陈思宇,徐杉,等. 背俞穴研究进展[J]. 中国中医药信息杂志,2009,16(S1):106109.

相似文献/References:

[1]李小琴,高玉峰,于跃,等.电针对大鼠脊髓中抑制性生长因子的影响[J].环球中医药,2013,6(09):653.
　LI Xiao qin,GAO Yu feng,YU Yue,et al.Investigation of electro acupuncture therapy on inhibitory growth factor in rat spinal cord[J].,2013,6(05):653.
[2]武燕,李瑞,田环环,等.电针胃脘下俞对2型糖尿病模型大鼠血糖及血脂影响的实验研究[J].环球中医药,2013,6(10):737.
　WU Yan,LI Rui,TIAN Huan huan,et al.Experiment research on electroacupuncture of “Weiwanxiashu” affecting the content of blood sugar and Bloodlipoids in Type 2 Diabetes Rats[J].,2013,6(05):737.
[3]王天怡,李佳楠,宋坪,等.电针配合穴位注射治疗带状疱疹后遗神经痛30例[J].环球中医药,2013,6(11):865.
[4]林驰,王寅,叶永铭,等.电针治疗阻塞性睡眠呼吸暂停低通气综合征的P300临床研究[J].环球中医药,2012,5(02):108.
　LIN Chi,WANG Yin,YE Yong-ming..Observation on the electroacupuncture in treating obstructive sleep apnea hypoventilation syndrome and its impact on the P300 test[J].,2012,5(05):108.
[5]李胜杰 李志同 杨伟宁等.电针上巨虚穴、尺泽穴对溃疡性结肠炎大鼠肺、肠肿瘤坏死因子 α 及结肠病理的比较研究[J].环球中医药,2014,7(06):415.
　LI Sheng jie,LI Zhi tong,YANG Wei ning,et al.Comparative study of electroacupuncture at Shangjuxu (ST 37) and Chize (LU 5) on TNFalpha content in the lung and colon and morphology of colonic mucosa in rats with ulcerative colitis[J].,2014,7(05):415.
[6]姚海江 莫雨平 宋洪涛等.电针对慢性应激抑郁模型大鼠松果体AANAT mRNA、HIOMT mRNA表达的影响[J].环球中医药,2014,7(07):511.
　YAO Hai-jiang,MO Yu-ping,SONG Hong-tao,et al.The effect of electro-acupuncture on the expression of AANAT mRNA and HIOMT mRNA in pineal of chronic stress depression model rats[J].,2014,7(05):511.
[7]加吾拉·阿不力孜,王鑫,李芙等.观察电针“百会”、“涌泉”两穴对不同月龄APP/PS1 双转基因小鼠海马Aβ淀粉样沉淀及超微结构的 影响[J].环球中医药,2015,8(05):518.[doi:10.3969/j.issn.16741749.2015.05.002]
　Jiawula·Abulizi,WANG Xin,LI Fu,et al.Effect of electroacupuncture intervention on hippocampus Aβ stain and ultrastructure in APP/PS1 double transgenic rats[J].,2015,8(05):518.[doi:10.3969/j.issn.16741749.2015.05.002]
[8]于淼 张丹妹 邬继红 图娅 史榕荇 许明敏 郭郁 张旭辉 王瑜 张春涛 赵冰骢.电针对慢性应激抑郁模型大鼠海马神经血管生成相关蛋白的表达影响[J].环球中医药,2016,9(04):385.[doi:10.3969/j.issn.1674-1749.2016.04.001]
　YU Miao,ZHANG Dan-mei,WU Ji-hong,et al.Influence of electroacupuncture on the expression of related proteins in the hippocampus of rats with chronic stress induced depression[J].,2016,9(05):385.[doi:10.3969/j.issn.1674-1749.2016.04.001]
[9]赵正芳 唐纯志.电针关元、三阴交对痛经模型大鼠血清IL-2、5-HT及P物质的影响[J].环球中医药,2017,10(05):541.[doi:10.3969/j.issn.1674-1749.2017.05.007]
　ZHAO Zhengfang,TANG Chunzhi..Effect of electroacupuncture at Guanyuan(CV4)and Sanyinjiao(SP6)on serum IL-2, 5-HT and substance P in dysmenorrhea model rats[J].,2017,10(05):541.[doi:10.3969/j.issn.1674-1749.2017.05.007]

备注/Memo

备注/Memo:
基金项目：国家自然科学基金(81141120) 作者单位：100029，北京中医药大学针灸推拿学院\[彭园园(硕士研究生)、张莉、刘通（博士研究生）、陈玉佩（硕士研究生）\]，基础医学院（葛东宇），科研实验中心（毛颖秋） 作者简介：彭园园（1988- ），女，2012级在读硕士研究生。研究方向：针灸临床机理研究。Email：175808605@qq.com 通讯作者：张莉（1957- ），女，博士，博士生导师，教授。研究方向：针灸临床机理研究。Email：zhangli1572@sina.com
更新日期/Last Update: 1900-01-01