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[1]屈岭 梁晓春 顾蓓 张宏 石玥.槲皮素通过自噬途径减轻高糖致体外培养RSC96细胞的损伤[J].环球中医药,2015,8(06):687-691.[doi:doi:10.3969/j.issn.1674-1749.2015.06.012]
　QU Ling,LIANG Xiao-chun,GU Bei,et al.Quercetin decreased RSC96 cells apoptosis induced by high glucose and increased proliferation via the pathway of autophagy[J].,2015,8(06):687-691.[doi:doi:10.3969/j.issn.1674-1749.2015.06.012]
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槲皮素通过自噬途径减轻高糖致体外培养RSC96细胞的损伤()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年06期
页码:
687-691
栏目:
论著
出版日期:
2015-06-06

文章信息/Info

Title:
Quercetin decreased RSC96 cells apoptosis induced by high glucose and increased proliferation via the pathway of autophagy
作者:
屈岭 梁晓春 顾蓓 张宏 石玥
100730 北京,中国医学科学院 北京协和医学院 北京协和医院中医科(屈岭、梁晓春、石玥),基础医学研究所细胞中心(顾蓓、张宏)
Author(s):
QU Ling; LIANG Xiao-chun; GU Bei; et al.
Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100730,China
关键词:
自噬;  凋亡;  槲皮素;  雪旺细胞;  高浓度葡萄糖
Keywords:
Autophagy;  Apoptosis;  Quercetin;  Schwann cell;  High glucose
分类号:
R285.5
DOI:
doi:10.3969/j.issn.1674-1749.2015.06.012
文献标志码:
A
摘要:
目的 观察槲皮素对高浓度葡萄糖培养的大鼠雪旺细胞系细胞(rat Schwann cell line,RSC96)自噬、增殖活性和凋亡的影响。方法 高浓度葡萄糖(125 mmol/L)及槲皮素(25 μmol/L)离体培养RSC96细胞,作用72小时后,噻唑基四唑(methyl thiazolyl tetrazolium,MTT)法检测细胞增殖活性,原位末端标记法[terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling,TUNEL]评价凋亡,Western blot法检测caspase-9活化的P35亚基、caspase-3活化的P20亚基及自噬相关蛋白Beclin1的蛋白表达。结果 高糖抑制离体培养的RSC96细胞的增殖活性,吸光度(optical density,OD)值降低,凋亡率增加,P35亚基、P20亚基蛋白表达上调(P<0.05,P<0.01); 槲皮素能够增加高糖培养RSC96细胞的增殖活性,下调P35、P20蛋白表达及凋亡率(P<0.05,P<0.01)。然而当自噬被抑制后,槲皮素上述保护作用则被削弱,细胞死亡增多(P<0.01)。结论 槲皮素通过增加自噬以促进RSC96细胞的增殖、减少凋亡,从而减轻高浓葡萄糖的损伤。
Abstract:
Objective To observe the protective effect of quercetin on the RSC96 cells cultured in high glucose in the aspects of autophagy, proliferation and apoptosis. Methods RSC96 cells were cultured with high glucose or quercetin media.The proliferation was detected by MTT method; the apoptosis was detected by TUNEL method; the expression of the activated subunit P35 of caspase-9 and the activated subunit P20 of caspase-3 were detected by Western blot method. Results The increased concentration of glucose lowered the proliferation of RSC96 cells and increased the apoptosis(P<0.05, P<0.01). Quercetin significantly up-regulated the proliferation rate, and down-regulated the apoptosis by attenuated the high glucose-induced activation of caspase-3 and caspase-9(P<0.05, P<0.01). Furthermore, inhibition of autophagy led to significantly attenuate the protective effect of quercetin(P<0.01). Conclusions Quercetin decreased apoptosis and up-regulated proliferation on the RSC96 cells cultured in high glucose through the autophagy pathway.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:屈岭(1980- ),博士,主治医师。研究方向:糖尿病及其慢性并发症的中西医诊治。E-mail:quling@pumch.cn 通讯作者:梁晓春(1956- ),女,硕士,教授,博士生导师。研究方向:糖尿病及其慢性并发症的中西医诊治。E-mail:xcliang@vip.sina.com
更新日期/Last Update: 1900-01-01