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[1]余春霞,顾选,李妍芃,等.基于DNA条形码—产地—形态分析联用的巴西草药BARDANA（牛蒡）的品种鉴定[J].环球中医药,2015,8(11):1298-1300.[doi:10.3969/j.issn.1674-1749.2015.11.006]
　YU Chun xia,GU Xuan,LI Yan peng,et al.Research on the source of varieties and high performance liquid chromatography of Brazilian herb BARDANA based on a combined analysis of DNA barcodingoriginmorphology[J].,2015,8(11):1298-1300.[doi:10.3969/j.issn.1674-1749.2015.11.006]
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基于DNA条形码—产地—形态分析联用的巴西草药BARDANA（牛蒡）的品种鉴定()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年11期
页码:
1298-1300
栏目:
巴西草药研究专题
出版日期:
2015-11-06

文章信息/Info

Title:
Research on the source of varieties and high performance liquid chromatography of Brazilian herb BARDANA based on a combined analysis of DNA barcodingoriginmorphology
作者:
余春霞; 顾选; 李妍芃; 肖瑶; 赵百孝; 刘春生; 马长华; 韩丽
450004郑州，河南省药品审评认证中心(余春霞)；北京华邈中药工程技术开发中心（顾选）；北京中医药大学中药学院[李妍芃（硕士研究生）、肖瑶、刘春生、马长华]，针灸推拿学院（赵百孝），养生学研究所（韩丽）
Author(s):
YU Chunxia;  GU Xuan;  LI Yanpeng;  et al.
Henan Province for Drug Evaluation Certification Center, Zhengzhou 450004, China
关键词:
巴西草药; 牛蒡; 品种鉴定; 牛蒡苷
Keywords:
Brazilian herb; BARDANA; Source of varieties; Arctiin
分类号:
R284
DOI:
10.3969/j.issn.1674-1749.2015.11.006
文献标志码:
A
摘要:
目的对巴西草药牛蒡的品种进行鉴定，同时研究其中的牛蒡苷含量，为其品种整理和药理研究提供依据。方法利用DNA条形码—产地—形态分析联用技术鉴定巴西草药牛蒡的基原，高效液相色谱法测定其牛蒡苷含量。结果DNA条形码初步鉴定巴西草药牛蒡为菊科牛蒡属植物，且与牛蒡达到最高相似度99%，产地及形态分析进一步确证其为牛蒡的地上部分；巴西牛蒡草中牛蒡苷平均含量为0.281%，明显低于中国牛蒡草牛蒡苷平均含量3.72%。结论巴西草药牛蒡来源于牛蒡Arctium lappa L.的干燥地上部分，其牛蒡苷含量极低。
Abstract:
ObjectiveTo trace the source and study on the content of arctiin of Brazilian herb BARDANA for varieties systematics and pharmacological analysis. MethodsThe source of varieties of Brazilian herb BARDANA was identified by a technology combined DNA barcoding, plant geographical origin and morphological features , and the content of arctiin was analyzed by HPLC. ResultsDNA barcoding preliminarily identified BARDANA belongs to the stretch of Arctium of Compositae with a similarity level of 99%. Geographical and morphological verification further confirmed the aforementioned finding. The content of arctiin in the Brazilian was 0.281%,which was significantly lower than the Chinese arctiin (3.72%). ConclusionBrazilian herb BARDANA derives from Arctium lappa L., and the content of arctiin was very low.

参考文献/References:

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更新日期/Last Update: 1900-01-01