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[1]祝日荣,任小巧,毛萌,等.藏医白脉疗法对局灶性脑缺血再灌注大鼠脑损伤的保护作用及对海马齿状回Jagged1表达的影响[J].环球中医药,2016,9(06):645-649.[doi:10.3969/j.issn.1674-1749.2016.06.001]
　ZHU Ri-rong,REN Xiao-qiao,MAO Meng,et al.Protective effects of the Tibetan Baimai(BM)therapy on focal cerebral ischemia-reperfusion injury in rats and the influence of BM therapy on Jagged1 expression of hippocampal dentate gyrus[J].,2016,9(06):645-649.[doi:10.3969/j.issn.1674-1749.2016.06.001]
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藏医白脉疗法对局灶性脑缺血再灌注大鼠脑损伤的保护作用及对海马齿状回Jagged1表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年06期
页码:
645-649
栏目:
论著
出版日期:
2016-06-06

文章信息/Info

Title:
Protective effects of the Tibetan Baimai(BM)therapy on focal cerebral ischemia-reperfusion injury in rats and the influence of BM therapy on Jagged1 expression of hippocampal dentate gyrus
作者:
祝日荣; 任小巧; 毛萌; 王明强; 郭慧娟; 仁青加; 葛东宇; 李根茂; 郑丽娟
100029 北京中医药大学基础医学院[祝日荣(硕士研究生)、王明强(硕士研究生)、郭慧娟(硕士研究生)、葛东宇、李根茂、郑丽娟(硕士研究生)]; 北京中医药大学民族医药学研究所(任小巧、毛萌); 西藏自治区西藏藏医学院(仁青加)
Author(s):
ZHU Ri-rong;  REN Xiao-qiao;  MAO Meng;  et al.
Basic Medical College,Beijing University of Chinese Medicine,Beijing 100029,China
关键词:
白脉疗法;  Jagged1;  海马齿状回;  局灶性脑缺血再灌注模型
Keywords:
Baimai therapy;  Jagged1;  Hippocampal dentate gyrus;  Focal cerebral ischemia-reperfusion model
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2016.06.001
文献标志码:
A
摘要:
目的 探讨白脉疗法对局灶性脑缺血再灌注大鼠脑损伤的保护作用及对海马齿状回Jagged1表达的影响。方法 线栓法制作局灶性脑缺血再灌注大鼠模型。随机分为假手术组、模型组、白脉疗法组,脑缺血1.5 小时,分别再灌注7 天、14 天,通过氯化三苯基四氮唑(triphenyltetrazolium chloride,TTC)染色观察脑梗死面积,苏木精-伊红染色法(hematoxylin-eosin staining,HE)染色观察皮层细胞形态变化,并应用免疫组化法检测各组大鼠海马齿状回Jagged1阳性细胞数量。结果(1)TTC染色观察到正常脑组织呈鲜红色,梗死区脑组织呈苍白色,并且随着再灌注时间的延长可见梗死区扩大,白脉疗法组的脑梗死体积小于模型组,且14天时与模型组比较有显著性差异(P<0.05)。(2)HE染色表明白脉疗法组脑缺血大鼠皮层神经元的损伤程度低于模型组。(3)白脉疗法可以上调脑缺血大鼠海马齿状回SGZ的Jagged1表达,术后7 天、14 天Jagged1阳性细胞数高于模型组、假手术组,且有显著性差异(P<0.05)。结论 白脉疗法对局灶性脑缺血再灌注大鼠脑损伤有一定的保护作用,且可促进大鼠局灶性脑缺血再灌注损伤后海马齿状回区Jagged1的表达,Jagged1的表达增加可能是白脉疗法通过Notch通路促进脑缺血损伤后内源性神经干细胞增殖分化的分子机制之一。
Abstract:
Objective To explore the protective effects of the Baimai(BM)therapy on focal cerebral ischemia-reperfusion injury in rats and the influence of BM therapy on Jagged1 expression of hippocampal dentate gyrus. Methods The focal cerebral ischemia-reperfusion injury models were induced by suture method, inserting thread 1.5 h and reperfusion 7 d and 14 d respectively. The rats were randomly divided into sham-operation group, model group, Baimai therapy group(BMT group). The area of cerebral infarction was observed by TTC staining, the morphological changes of cortex cell was observed by HE staining, and immunohistochemistry method was used to detect hippocampal dentate gyrus Jagged1 positive cell number of each group rats. Results(1)the TTC staining result showed that normal brain tissue was bright red, infarction area of the brain was pale, and with the prolongation of reperfusion time, the infarct area was enlarged. The areas of cerebral infarction of BMT group were less than model group, and there was a significant difference compared with model group on 14 days(P<0.05).(2)HE staining showed that degree of injury of cortical neurons in the cerebral ischemia group was lower than that in the model group.(3)BM therapy can increase hippocampal dentate gyrus Jagged1 expression of cerebral ischemia rats, Jagged1 positive cells was higher than model group and control group, the result had significant difference(P<0.05), after having operation 7 days and 14 days. Conclusions BM therapy has certain protective effect on focal cerebral ischemia-reperfusion injury, and can promote Jagged1 expression in hippocampal dentate gyrus after rats having focal cerebral ischemia-reperfusion injury, the increase of Jagged1 expression may be one of the molecular mechanisms of the proliferation and differentiation of endogenous neural stem cells after cerebral ischemia injury via the Notch pathway.

参考文献/References:

[1] 仁旺次仁, 扎桑. 藏医白脉疗法在中风病治疗康复中的作用[J].中国藏学, 2010,(4): 159-162.
[2] 毛萌, 任小巧, 仁青加. 浅议藏医白脉疗法对脑卒中后遗症的治疗特色[J].中医药学报, 2014, 42(3): 7-9.
[3] 索朗顿珠. 藏汉合璧常用藏成药使用手册[M]. 北京:民族出版社,2006: 436,439-440.
[4] 刘宪勇, 王丽娥, 付加雷. 如意珍宝丸治疗脑中风后遗症临床观察[J].山东医药, 2010, 50(1): 88.
[5] 李宁, 郭文华, 董纪革. 白脉软膏改善脑卒中患者肌张力增高的有效性和安全性临床研究[J].中国实用医药, 2011, 6(35):240-242.
[6] Lin Jen-tsun, Chen Mu-kuan, Yeh Kun-tu. Association of High Levels of Jagged-1 and Notch-1 Expression with Poor Prognosis in Head and Neck Cancer[J]. Annals of Surgical Oncology, 2010, 17: 2976
[7] Belayev L, Alonso OF, Busto R, et al. Middle cerebral artery occlusion in the rat by intraluminal suture. Neurological and pathological evaluation of an improved model.[J]. Stroke, 1996,27(9):1616-1623.
[8] Zea Longa E, Weinstein PR, Carlson S, et al. Middle cerebral artery occlusion without craniectomy in rats[J]. Stroke, 1989,20:84-91.
[9] 翁维良.中药临床药理学[M].北京:人民卫生出版社,2002:40.
[10] George Paxinos, Charles Watson, 诸葛启钏.大鼠脑立体定位图谱[M]. 北京: 人民卫生出版社,2005:99.
[11] 袁栋才, 张凤春, 魏巍. 神经可塑性与脑卒中后运动功能恢复的研究进展[J].中国康复医学杂志, 2014,29(4): 391.
[12] Rebecca D Hodge, Robert JKahoud, Robert F Hevner. Transcriptional control of glutamatergic differentiation during adult neurogenesis[J]. Cellular and Molecular Life Sciences, 2012, 69(13): 2125-2134.
[13] AguirreAdan, Rubio Maria E, Gallo Vittorio. Notch and EGFR pathway interaction regulates neural stem cell number and self-renewal[J]. Nature, 2010,467(7313): 323-327.
[14] Nyfeler Yves, Kirch Robert D, Mantei Ned, et al. Jagged1 signals in the postnatal subventricular zone are required for neural stem cell self-renewal[J].EMBO Journal, 2005,24(19): 3504-3515.
[15] 韩羽楠,王振宇. Notch和Wnt信号通路对神经干细胞增殖分化的影响[J]. 解剖科学进展, 2014, 20(4): 385-387.
[16] 闫荣, 罗晓光, 张尧, 等. 星形胶质细胞影响神经干细胞突触表达的神经营养家族基因表达机制探讨[J]. 中国医科大学学报, 2013,42(11): 989, 992-995.
[17] FreddyJeanneteau, Katrin Deinhardt, Goichi Miyoshi, et al. The MAP kinase phosphatase MKP-1 regulates BDNF-induced axon branching[J]. Nature Neuroscience, 2010, 13(11): 1373-1379.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金(81360575)
作者简介: 祝日荣(1989- ),女,2013级在读硕士研究生。研究方向:中医诊断学。 E-mail: zhurirong1 @163.com
通讯作者: 任小巧(1965- ),女,博士,教授。研究方向:民族医药与现代疾病的证治规律研究。E-mail: rxq23111111@126.com
更新日期/Last Update: 2016-06-06