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[1]吴欢欢,周爱军,胡丽娟,等.加味小柴胡汤及其有效物质部位群抗炎实验研究[J].环球中医药,2016,9(06):664-667.[doi:10.3969/j.issn.1674-1749.2016.06.005]
　WU Huan-huan,ZHOU Ai-jun,HU Li-juan,et al.Experimental study on anti-inflammatory effect of modified Xiaochaihu decoction and its effective fractions[J].,2016,9(06):664-667.[doi:10.3969/j.issn.1674-1749.2016.06.005]
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加味小柴胡汤及其有效物质部位群抗炎实验研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第9卷
期数:
2016年06期
页码:
664-667
栏目:
论著
出版日期:
2016-06-06

文章信息/Info

Title:
Experimental study on anti-inflammatory effect of modified Xiaochaihu decoction and its effective fractions
作者:
吴欢欢; 周爱军; 胡丽娟; 陈雪剑; 杨梦玲; 刘焱文
430060 武汉,湖北中医药大学湖北省中药资源与中药化学重点实验室[吴欢欢(硕士研究生)、胡丽娟、陈雪剑、杨梦玲(硕士研究生)、刘焱文]; 东莞市中医院中医药研究所(周爱军)
Author(s):
WU Huan-huan;  ZHOU Ai-jun;  HU Li-juan;  et al.
Provincial Key Laboratory of Traditional Chinese Medicine Resources and Traditional Chinese Medicine Chemistry of Hubei University of Chinese Medicine, Wuhan 430070,China
关键词:
加味小柴胡汤;  有效物质部位群;  抗炎
Keywords:
Modified Xiaochaihu decoction;  Effective fractions;  Anti-inflammatory
分类号:
R285
DOI:
10.3969/j.issn.1674-1749.2016.06.005
文献标志码:
A
摘要:
目的 对加味小柴胡汤及其有效物质部位群进行抗炎活性实验研究,确定其有效物质部位群,以期阐述其药效物质基础,为该经典方剂治疗慢性胃炎的临床疗效提供科学实验依据。方法 将加味小柴胡汤复方视为整体,采用系统溶剂法将其提取分离为挥发油、多糖、生物碱、酚酸类、皂苷类等化学物质部位; 采用二甲苯致小鼠耳廓肿胀和角叉菜胶致大鼠足趾肿胀抗炎实验动物模型,对该方剂及其各物质部位进行抗炎实验研究。结果 二甲苯致小鼠耳廓肿胀模型实验研究结果表明,与模型组比较,全方高及低剂量组、皂苷部位高及低剂量组、多糖部位高剂量组均能极显著抑制小鼠耳肿胀(P<0.01); 酚酸类物质部位高剂量组能显著抑制小鼠耳肿胀(P<0.05)。角叉菜胶致大鼠足趾肿胀模型实验结果表明,与模型组比较,全方高及低剂量组、皂苷部位高及低剂量组、酚酸类物质部位高剂量组均能极显著地减轻大鼠足趾肿胀(P<0.01); 多糖部位高剂量组能显著减轻大鼠足趾肿胀(P<0.05)。结论 确定了加味小柴胡汤及其有效物质部位群的抗炎作用,从而为该临床经验方治疗慢性胃炎提供了现代药理学实验依据。
Abstract:
Objective To investigate the anti-inflammatory effect of modified Xiaochaihu decoction and its substance fractions. To determine the effective material parts, in order to explain its pharmacodynamic material basis, and to provide a scientific basis for the treatment of gastritis. Methods The means of systematic solvent extraction was used to separate volatile oils, polysaccharides, alkaloids, phenolic acids and saponins from the decoction; animal were divided into high and low dose groups. Mouse ear swelling induced by xylene and the rats paw edema induced by carrageenin to establish the animal model of anti inflammatory, the anti-inflammatory effect of modified Xiaochaihu decoction and each substance fractions was investigated. Results Xylene-induced mouse ear swelling model, compared with model group,the decoction high and low dose group, saponins high and low dose group, polysaccharide high dose group can significantly inhibit the mouse ear edema(P<0.01); phenolic acids part of the high dose group could significantly inhibit the mouse ear edema(P<0.05). Carrageenan-induced rat paw edema test, compared with model group, the decoction high and low dose group, saponins high and low dose group, phenolic acids high dose group can significantly inhibit the mouse ear edema(P<0.01), polysaccharides high dose group can significant reduce toes swelling of rats(P<0.05). Conclusion The anti-inflammatory effect of modified Xiaochaihu decoction and its effective material site group was determined,which provided modern pharmacology experiment basis for the classic prescription of the clinical treatment of chronic gastritis.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 广东省东莞市科技局项目(2013108101036)
作者简介: 吴欢欢(1990- ),女,2013级在读硕士研究生。研究方向:中药及其制剂的物质基础研究。E-mail:260617673@qq.com
通讯作者: 刘焱文(1945- ),本科,教授,博士生导师。研究方向:中药及其制剂的物质基础研究。E-mail:ywliu2008@163.com
更新日期/Last Update: 2016-06-06