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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:

第9卷

期数:

2016年08期

页码:

905-908

栏目:

论著

出版日期:

2016-08-06

文章信息/Info

Title:

Protective effects of active part of Sagittaria Sagittifolia L. on hepatocytes injury in vitro

作者:

李冰 林殷 王春国 王晶 奚茜 潘诗霞 马芳芳 吕威 廖艳

100029 北京中医药大学基础医学院[李冰(硕士研究生)、林殷、王晶(硕士研究生)、奚茜(硕士研究生)、潘诗霞(硕士研究生)、马芳芳(博士研究生)、廖艳],科研实验中心(王春国); 针灸推拿学院[吕威(硕士研究生)]

Author(s):

LI Bing;  LIN Yin;  WANG Chun-guo;  et al.

School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China

关键词:

慈姑活性成分;  异烟肼;  利福平;  肝损伤

Keywords:

Active part of Sagittaria Sagittifolia L.;  Isoniazid;  Rifampicin;  Hepatocytes Injury

分类号:

R285.5

DOI:

10.3969/j.issn.1674-1749.2016.08.001

文献标志码:

A

摘要:

目的 研究慈姑活性成分的保肝作用及其机理。方法 采用系统化学溶剂萃取法制备慈姑不同活性成分,培养HepG2型肝细胞,建立异烟肼和利福平合用致体外肝细胞损伤模型,给予慈姑不同活性成分处理后,利用MTT法检测肝细胞的存活率,并检测细胞培养上清液中丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、乳酸脱氢酶、丙二醛的浓度。结果 与模型组比较,慈姑乙酸乙酯组、慈姑石油醚组、慈姑总提取物组和慈姑水提组的细胞存活率均增加(P＜0.01); 慈姑石油醚组、慈姑总提取物组和慈姑水提组均可降低肝细胞培养上清液中的丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、乳酸脱氢酶、丙二醛的水平(P＜0.05),其中慈姑水提组效果最为明显(P＜0.01)。结论 提示慈姑水提部分对体外异烟肼和利福平合用致肝细胞损伤有较强保护作用,其作用可能与抗脂质过氧化有关。

Abstract:

Objective To study the protective effects and mechanism of active components of Sagittaria Sagittifolia L. on hepatocytes injury induced by co-administration of isoniazid and rifampin. Methods Different active ingredients of Sagittaria Sagittifolia L. were prepared by systemic chemical solvent extraction method. HepG2 liver cells were incubated, and injured by co-administration of isoniazid and rifampin. MTT assay was used to evaluate the survival rate of liver cells after the cells had been treated with active part of Sagittaria Sagittifolia L., culture media were collected to detected the levels of ALT, AST, LDH and MDA. Results Compared with the model group, the cell viability of ethyl acetate extract group, petroleum ether extract group, the total extract group and the water extract group was increased(P＜0.01); the content of ALT, AST, LDH and MDA was reduced after preventive treatment with petroleum ether extract, the total extract and the water extract, the effect of water extract group was especially obvious(P＜0.01).Conclusion The water extract from Sagittaria Sagittifolia L. has strong protective effect on hepatocytes injury induced by co-administration of isoniazid and rifampin, which may be related to inhibiting the lipid peroxidation.

参考文献/References:

[1] 滕光菊,孙颖,常彬霞,等.418例药物性肝损害临床特征及预后分析[J].肝脏,2013,18(1):11-14.
[2] 郑晓丹,洪丽如,陈瑞烈.抗结核药所致药物性肝损伤80例临床分析[J].热带医学杂志,2015,15(4):536-538.
[3] 潘晓军,刘芬.慈姑的研究进展[J].西北药学杂志,2006,21(3):146.
[4] 陆祖福,吴小南,汪家梨,等.慈菇营养素成分分析与抗氧化作用机理探讨[J].海峡预防医学杂志,2002,8(1):50-51.
[5] 廖艳,孙奇,彭桂英,等.慈菇对异烟肼和利福平致大鼠肝损伤的保护作用[J].北京中医药大学学报,2012,35(7):466-469.
[6] 陆祖福,吴小南,汪家梨,等.慈菇对镉致急性肝脏过氧化损伤影响的研究[J].中国公共卫生,2002,18(4):388-389.
[7] 吴小南,汪家梨.慈菇对小鼠四氯化碳肝损伤保护作用的探讨[J].中国公共卫生学报,1996,15(4):238-239.
[8] Lian Y, Zhao J,Xu PY,et al.Protective effects of metallothionein on isoniazid and rifampicin-induced hepatotoxicity in mice[J].PLoS One,2013,8(8):e72058.
[9] 段自皞.异烟肼和利福平肝脏毒性研究进展[J].现代医学,2013,41(6):430-433.
[10] 吴小南,陆祖福,黄芳,等.一氧化氮在镉致急性肝损伤中的作用及慈菇的干预效果[J].中国公共卫生,2002,18(8):907-908.
[11] 吴小南,杨雪帆,朱萍萍,等.慈菇多糖对免疫功能低下小鼠免疫调节作用[J].中国公共卫生,2015,31(1):73-75.
[12] 张翠香,罗永会,徐春萍.野生慈姑多糖对糖尿病小鼠血糖和血脂作用研究[J].考试周刊,2013,(27):194-195.

相似文献/References:

[1]杨铂,刘靓雯,王小利,等.解毒护肝饮药物血清对异烟肼致HepG2坏死和凋亡的干预[J].环球中医药,2012,5(04):259.
　YANG Bo,LIU Jing wen,WANG Xiao li,et al.Effect of Jiedu Hugan Decoction containing serum on the death and apoptosis of HepG2 which were damaged by isoniazid[J].,2012,5(08):259.

备注/Memo

备注/Memo:

基金项目: 国家自然科学基金(81503633); 北京中医药新奥奖励基金(1000062720044/004)
作者简介: 李冰(1991- ),女,2014级在读硕士研究生。研究方向:中医养生与康复。E-mail:1145571606@qq.com
通讯作者: 廖艳(1975- ),女,博士,副教授,硕士生导师。研究方向:药理毒理学。E-mail:liaoyanaa@163.com

常用功能

更新日期/Last Update: 2016-08-06