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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:

第9卷

期数:

2016年11期

页码:

1289-1292

栏目:

浊毒研究专题

出版日期:

2016-11-06

文章信息/Info

Title:

Protective effect of Changpu Yizhi compound decoction on cerebral ischemia and reperfusion injury in mice

作者:

徐丽娟 许斐 臧春柳 赵层闪 刘学飞 杨丽静 田军彪

050011 石家庄,河北省中医院脾胃病科(徐丽娟、刘学飞、杨丽静、田军彪、); 河北省晋州市人民医院脾胃病科(许斐); 河北省唐县中医院脾胃病科(臧春柳); 河北省衡水市中医院脾胃病科(赵层闪)

Author(s):

XU Li-juan;  XU Fei;  ZANG Chun-liu;  et al.

Traditional Chinese Medicine Hospital of Hebei Province, Shijiazhuang 050011, China

关键词:

脑缺血再灌注损伤;  复方菖蒲益智汤;  Ca²⁺-ATP酶;  Na⁺-K⁺-ATP酶

Keywords:

Cerebral ischemia and reperfusion injury;  Changpu Yizhi compound decoction;  Ca²⁺-ATPase;  Na⁺-K⁺-ATPase

分类号:

R285.5

DOI:

10.3969/j.issn.1674-1749.2016.11.001

文献标志码:

A

摘要:

目的 观察复方菖蒲益智汤对小鼠脑缺血再灌注损伤早期脑组织中Ca²⁺-ATP酶及Na⁺-K⁺-ATP酶活性的影响。方法 实验小鼠随机分为假手术组、模型组、复方菖蒲益智汤高、低剂量组、尼莫地平对照组。采用夹闭双侧颈总动脉的方法制备脑缺血再灌注损伤模型小鼠,给药14天后进行神经功能损伤评分,并采用HE染色,光镜下观察海马区脑组织的病理形态学变化,测定脑组织Ca²⁺-ATP酶及Na⁺-K⁺-ATP酶活性。结果 各用药组小鼠海马区锥体细胞损伤程度、神经功能缺损程度比模型组均有所减轻,以复方菖蒲益智汤高剂量组效果最为明显,高剂量组小鼠的Ca²⁺-ATP酶及Na⁺-K⁺-ATP酶活性高于模型组、低剂量组及尼莫地平组(P<0.01)。结论 复方菖蒲益智汤能够提高脑缺血再灌注小鼠脑组织Ca²⁺-ATP酶及Na⁺-K⁺-ATP酶活性,对于小鼠脑缺血再灌注损伤有保护作用。

Abstract:

Objective To observe the effect of Changpu Yizhi compound decoction on the activity of Ca²⁺-ATPase and Na⁺-K⁺-ATPase in brain tissue of mice in the early stage of cerebral ischemia and reperfusion injury. Methods Mice were randomly divided into sham operation group, model group, high and low doses of Changpu Yizhi compound decoction group and nimodipine control group. Cerebral ischemia and reperfusion injury(CIRI)mice model was prepared through blocking both side carotid arteries. After continuous medication for 14 days, neurological injury score was evaluated, and pyramidal cell morphology of the hippocampal region was observed under light microscope after HE staining. The activity of the Ca²⁺-ATPase and Na⁺-K⁺-ATPase in the brain tissue was detected. Results Compared with the model group, the injury of hippocampal pyramidal cells of each medical group was alleviated and neurological function deficit was lowered, especially in the high dose group. The activity of the Na⁺-K⁺-ATPase and Ca²⁺-ATPase in the high dose group was higher than that of the other groups except the sham operation group(P<0.01). Conclusion Changpu Yizhi compound decoction could enhance the activity of the Na⁺-K⁺-ATPase and Ca²⁺-ATPase in mice with cerebral ischemia and reperfusion injury, indicated its protective effect on the injury.

参考文献/References:

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备注/Memo

备注/Memo:

基金项目: 河北省自然科学基金面上项目(H2013206393); 河北省科技支撑计划(15277748D); 河北省省级中医药类重大医学科研课题(zyzd2013004); 河北省中医药管理局科研计划(2014039); 河北省政府资助临床医学优秀人才培养和基础课题(361025)
作者简介: 徐丽娟(1978- ),女,硕士,主治医师。研究方向:神经及脑血管疾病诊治。E-mail:xlj_alice@126.com
通讯作者: 田军彪(1964- ),博士,教授。研究方向:脑血管疾病中医药防治研究。E-mail:jbt1965@sina.com

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更新日期/Last Update: 2016-11-06