|本期目录/Table of Contents|

[1]张玉,邵建国,陈琳,等.季德胜蛇药通过调控miR-335抑制肝癌Huh-7细胞增殖作用机制探讨[J].环球中医药,2017,10(04):452-456.[doi:10.3969/j.issn.1674-1749.2017.04.015]
　ZHANG Yu,SHAO Jianguo,CHEN Lin,et al.Mechanism discussion of JiDeSheng Sheyao inhibiting liver cancer cell Huh-7 proliferation through regulating miR-335[J].,2017,10(04):452-456.[doi:10.3969/j.issn.1674-1749.2017.04.015]
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季德胜蛇药通过调控miR-335抑制肝癌Huh-7细胞增殖作用机制探讨()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年04期
页码:
452-456
栏目:
论著
出版日期:
2017-04-06

文章信息/Info

Title:
Mechanism discussion of JiDeSheng Sheyao inhibiting liver cancer cell Huh-7 proliferation through regulating miR-335
作者:
张玉; 邵建国; 陈琳; 卞兆连; 刁花玉; 达坤林
226000 南通市第三人民医院中医科(张玉、达坤林),肝病研究所(邵建国、陈琳、卞兆连、刁花玉)
Author(s):
ZHANG Yu; SHAO Jianguo; CHEN Lin; et al.
The third people's Hospital of Nantong,Nantong 226000,China
关键词:
季德胜蛇药;  人肝癌细胞;  miR-335;  Bcl-w
Keywords:
Jidesheng Sheyao;  Huh-7;  miR-335;  Bcl-w
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.04.015
文献标志码:
A
摘要:
目的 探讨季德胜蛇药对肝癌Huh-7细胞增殖作用的影响并进一步从miRNA层面探讨其抑制增殖的调控机制。方法 以肝癌Huh-7细胞为模型,制备不同浓度的兔含药血清后培养Huh-7细胞,并同时设置相应浓度的对照组。CCK-8实验检测转染miR-335入Huh-7细胞后对该细胞增殖能力的影响; 采用实时定量聚合酶链式反应qRT-PCR检测季德胜蛇药含药血清对Huh-7细胞中miR-335的调控作用; CCK-8实验检测季德胜蛇药含药血清对Huh-7细胞增殖能力的影响; Western-blot检测miR-335潜在靶向Bcl-w表达水平的变化。结果 miR-335过表达后CCK-8实验组与空白对照组及阴性对照组相比,其OD值显著降低(P＜0.01)。含药血清干预下,与对照组相比,同等浓度的蛇药组miR-335表达水平较高,且在一定浓度范围内含药血清浓度越高,miR-335在Huh-7细胞中的表达也相继增高; 此外,CCK-8实验下季德胜蛇药含药血清组都较正常血清组OD值低,且浓度越高,其OD值越低,都有较明显的统计学差异(P＜0.01)。上调miR-335后,Huh-7细胞Bcl-w蛋白表达水平降低。结论 季德胜蛇药通过调控miR-335能抑制肝癌Huh-7细胞增殖。
Abstract:
Objective To discuss the mechanism of JiDeSheng Sheyao inhibiting liver cancer cell Huh-7 proliferation through regulating miRNA.Methods Cell Huh-7 were used as the model for study. Rabbits were given Jidesheng Sheyao by gastrogavage to prepare medicated serum. Then Huh-7 was cultured with medicated serum by different concentration. At the same time, the corresponding concentrations of the control group were set up. The effect of miR-335 on Huh-7 cells proliferation was tested by CCK-8 assay. QRT-PCR was used to detect the regulation function of miR - 335 on human liver cancer Huh-7 cell. The effect of Jidesheng Sheyao medicated serum on Huh-7 cells proliferation was tested by CCK-8 assay. The effect of miR-335 on potential target protein Bcl-w was tested by Western-blot. Results The OD value of the experimental group was decreased significantly compared with blank control group and the negative control group by CCK-8 assay after up-regulation of miR-335(P＜0.01).The miR-335 expression level was higher compared with the control group in the same concentration of snake medicine group. In a certain concentration range, the expression of miR-335 in Huh-7 cells increased with increasing serum concentration; In addition, the OD value of JiDeSheng Sheyao medicated serum group were lower compared with normal serum, and the concentration were higher, its OD value were lower by CCK 8 experimental. They had also a significant statistical difference(P＜0.01, P＜0.001). After raising miR-335, the Bcl-w protein expression levels in Huh-7 cells were lower. Conclusion JiDeSheng Sheyao can inhibite liver cancer Huh-7 cell proliferation through regulating miR-335.

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备注/Memo

备注/Memo:
基金项目: 江苏省卫生厅面上项目(H201453); 南通市社会事业科技创新与示范计划(HS2014061); 2014年度江苏省南通市市级应用研究计划(BK2014073); 南通市科技局:新型临床诊疗技术攻关(MS22015105)
作者简介: 张玉(1991- ),硕士,住院医师。研究方向:中西医结合消化内科。E-mail:1198963491@qq.com
通信作者: 邵建国(1965- ),博士,主任医师,教授。研究方向:消化系统疾病临床研究。E-mail:shaojiang
更新日期/Last Update: 2017-04-06