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[1]代一航,赵崇军,田敬欢,等.香加皮水提取物对斑马鱼幼鱼肝脏毒性的初步研究[J].环球中医药,2017,10(10):1061-1066.[doi:10.3969/j.issn.1674-1749.2017.10.001]
　DAI Yihang,ZHAO Chongjun,TIAN Jinghuan,et al.Effects of waterextract from cortex periplocae on hepatotoxicity of zebrafish[J].,2017,10(10):1061-1066.[doi:10.3969/j.issn.1674-1749.2017.10.001]
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香加皮水提取物对斑马鱼幼鱼肝脏毒性的初步研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年10期
页码:
1061-1066
栏目:
论著
出版日期:
2017-10-06

文章信息/Info

Title:
Effects of waterextract from cortex periplocae on hepatotoxicity of zebrafish
作者:
代一航; 赵崇军; 田敬欢; 倪媛媛; 李二文; 杨冉冉; 冯丹; 刘雯雪; 王昭懿; 乔艺涵; 马志强; 林瑞超; 邹迪新
100102 北京中医药大学中药学院中药品质评价北京市重点实验室[代一航(硕士研究生)、赵崇军(博士研究生)、田敬欢(硕士研究生)、倪媛媛(硕士研究生)、李二文(硕士研究生)、杨冉冉(硕士研究生)、冯丹(博士研究生)、刘雯雪(硕士研究生)、王昭懿(博士研究生)、乔艺涵(硕士研究生)、马志强、林瑞超]; 内蒙古医科大学药学院(邹迪新)
Author(s):
DAI Yihang;  ZHAO Chongjun;  TIAN Jinghuan;  et al.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing Key Laboratory for Quality Evaluation of Traditional Chinese Medicine,Beijing 100102,China
关键词:
香加皮;  水提取物;  斑马鱼模型;  肝脏毒性
Keywords:
Cortex periplocae;  Waterextract;  Zebrafish model;  Hepatotoxicity
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.10.001
文献标志码:
A
摘要:
目的 研究香加皮水提取物对斑马鱼幼鱼肝脏的毒性作用。方法 将受精后发育4天的斑马鱼幼鱼暴露于不同浓度的香加皮水提取物中,24小时后,统计幼鱼的死亡率,计算量毒曲线; 选取低于LC10的三个药物暴露组和空白组,以幼鱼肝脏形态和面积变化、转基因幼鱼肝脏荧光面积和荧光强度以及幼鱼肝脏细胞凋亡、超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化酶(glutathione peroxidase,GSH-Px)、丙氨酸转氨酶(alanine aminotransferase,ALT)、天门冬氨酸转氨酶(aspartic transaminase,AST)活性为毒性评价指标。结果 香加皮水提取物对幼鱼的量-毒回归曲线y=-1.0843+0.0014x(R2=0.9524,r=0.97590,P<0.001),LC10为845.9286 μg/mL; 与空白组相比,幼鱼肝脏形态异常、透明度降低,肝脏面积随药物暴露浓度增加而剂量依赖性增加(P<0.05); 转基因幼鱼肝脏荧光面积随暴露浓度增加而增大,但在高浓度组减小,荧光强度随暴露浓度的增加而减小(P<0.05); 高浓度暴露组幼鱼肝脏区域显示了细胞凋亡; 药物暴露组幼鱼SOD、GSH-Px活力显著下降,ALT、AST活力显著上升(P<0.05)。结论 香加皮水提取物对斑马鱼幼鱼的肝脏毒性可能是通过破坏其体内氧化应激平衡,进而诱导肝脏细胞凋亡实现的。
Abstract:
Objective To study the toxic effect of waterextract from cortex periplocae on liver of zebrafish larval. Methods Zebrafish embryos of 4 days post-fertilization were exposed to 12-well plates with different concentrations of waterextract from cortex periplocae for 24 hours, and the mortality rate was calculated and the toxic curve was calculated. Three drug exposed groups less than LC10 and the blank group were selected. The changes of liver morphology and area, fluorescence area and fluorescence intensity of liver, apoptosis of liver cell, superoxide dismutase, glutathione peroxidase, alanine aminotransferase, aspartic transaminase were used as indicator of toxicity assessment. Results The LC10 of waterextract from cortex periplocae was 845.9286 μg/mL,the mortality rate was dependent with the concentration, concentration-mortality curve was y=-1.0843+0.0014x(R2=0.9524,P<0.001),and correlation coefficient was 0.97590(P<0.001). Compared with the control group, morphological abnormalities was more and transparency was decreased in liver, and liver area was increased with drug exposure concentration(P<0.05); The fluorescence area was increased with the exposure concentration increased, but in the high concentration group, the fluorescence intensity was decreased with the exposure concentration increased(P<0.05); The apoptosis was appeared in high concentration exposed group. The activity of SOD, GSH-Px was significantly decreased while the activity of AST, ALT was significantly increased. Conclusion Waterextract from cortex periplocae can induce hepatotoxicity in zebrafish larvae, and the mechanism may relate to destroy the balance of oxidative stress and induce apoptosis in liver cells.

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　FENG Yaru,ZHAO Chongjun,NI Yuanyuan,et al.Effects of Cortex Periplocae on zebrafish developmental toxicity and cardiac toxicity[J].,2017,10(10):536.[doi:10.3969/j.issn.1674-1749.2017.05.006]

备注/Memo

备注/Memo:
基金项目:北京市科委创新环境与平台建设专项(Z16111000500000)
作者简介:代一航(1991- ),2015级在读硕士研究生。研究方向:中药毒性成分与中药药理研究。E-mail:2449416672@qq.com
通信作者:林瑞超(1954- ),博士,教授,博士生导师。研究方向:中药、民族药质量控制与品质评价研究。E-mail:linrch307@sina.com; 邹迪新(1983- ),女,博士,讲师。研究方向: 中药药效物质基础及作用机制研究。Email:zoudixin@163.com
更新日期/Last Update: 2017-10-06