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[1]刘晔,车念聪,杜宇琼,等.芪水煎对糖尿病肾病小鼠肝、肾组织细胞外基质调节因子表达的影响[J].环球中医药,2017,10(10):1070-1074.[doi:10.3969/j.issn.1674-1749.2017.10.003]
　LIU Ye,CHE Niancong,DU Yuqiong,et al.Effects of Qishui decoction on the expression of extracellular matrix regulatory factors in liver and kidney of diabetic nephropathy mice[J].,2017,10(10):1070-1074.[doi:10.3969/j.issn.1674-1749.2017.10.003]
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芪水煎对糖尿病肾病小鼠肝、肾组织细胞外基质调节因子表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年10期
页码:
1070-1074
栏目:
论著
出版日期:
2017-10-06

文章信息/Info

Title:
Effects of Qishui decoction on the expression of extracellular matrix regulatory factors in liver and kidney of diabetic nephropathy mice
作者:
刘晔; 车念聪; 杜宇琼; 王金光; 李文新
100069 北京,首都医科大学中医药学院中医临床基础学系[刘晔(硕士研究生)、车念聪、杜宇琼、王金光(硕士研究生)、李文新(硕士研究生)]
Author(s):
LIU Ye; CHE Niancong; DU Yuqiong; et al.
the Capital Medical University, traditional Chinese medicine,Beijing 100069, China
关键词:
黄芪;  水红花子;  肝肾同源;  肝损伤;  肾基质金属蛋白酶-9;  基质金属蛋白酶组织抑制因子-1
Keywords:
Astragalus membranaceus;  Fructus polygoni orientalis;  Homogeny of liver and kidney;  Hepatic injury;  Matrix metalloproteinase-9;  Tissue inhibitor of metalloproteinase-1
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2017.10.003
文献标志码:
A
摘要:
目的 基于肝肾同源理论,观察糖尿病肾病(kk-ay)小鼠后期谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶含量(aspartate transaminase,AST)变化以及芪水煎对肝、肾基质金属蛋白酶-9(Matrix metalloe proteinas-9,MMP-9)及基质金属蛋白酶组织抑制因子-1(tissue inhibitor of metalloproteinase-1,TIMP-1)表达的影响。 方法 采用kk-ay小鼠高糖高脂饮食4周建立糖尿病肾病模型后,随机分为模型组、黄芪水组、通心络组,设立C57BL/6J小鼠作为对照组。予芪水煎及通心络干预12周后,监测各组小鼠血ALT、AST,采用Western Blot、Realtime-PCR检测肝、肾组织MMP-9和TIMP-1蛋白含量及mRNA表达水平。 结果 与模型组比较,芪水煎组AST显著降低(P<0.01)、ALT呈下降趋势(P>0.05),肝MMP-9蛋白及mRNA表达显著升高(P<0.01),TIMP-1蛋白显著降低(P<0.01),mRNA表达降低(P<0.05),肾MMP-9蛋白及mRNA表达升高(P<0.05),TIMP-1蛋白及mRNA表达降低(P<0.05)。结论 kk-ay小鼠后期会出现肝损伤,芪水煎对kk-ay小鼠肝、肾损伤具有一定的保护作用。
Abstract:
Objective To investigate the content changes of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)of diabetic nephropathy(kk-ay)mice in later stage, and to investigate the influence of Qishui decoction on the expression of MMP-9, TIMP-1 in the hepatic and nephritic tissues based on the theory of “homogeny of liver and kidney”. Methods The diabetic nephropathy model was established by kk-ay mice with high glucose and high fat diet for 4 weeks. The mice were randomly divided into model group, Qishui group and Tongxinluo group, and C57BL/6J mice were used as control group. After 12 weeks of drug intervention, the level of ALT and AST was detected, and the protein and mRNA expressions of MMP-9 and TIMP-1 in liver and kidney were detected by Western Blot and realtime-PCR. Results Compared with the model group, the level of AST in Qishui group was significantly decreased(P<0.01), while the level of ALT was decreased(P>0.05). The protein and mRNA expression of MMP-9 in liver was significantly increased(P<0.01), while the protein expression of TIMP-1 was significantly decreased(P<0.01), and mRNA was decreased(P<0.05). The protein and mRNA expression of MMP-9 in kidney was increased(P<0.05), while the protein and mRNA expression of TIMP-1 was decreased(P<0.05). Conclusion kk-ay mice have liver injury in the later stage of diabetic nephropathy, and Qishui decoction has protective effects on liver and kidney injury in kk-ay mice.

参考文献/References:

[1] 高磊,邹志强,孙荣玲,等. 糖尿病在肝损伤患者中的危险性分析[J]. 中国医药导报,2012,9(30):30-32.
[2] 韩文兵. 基于“肝肾同源”理论探讨柴黄益肾颗粒防治糖尿病肝肾并发症的机制研究[D].北京:北京中医药大学,2013.
[3] Lakshmanan AP, Thandavarayan RA, Watanabe K, et al. Modulation of AT-1R/MAPK cascade by an olmesartan treatment attenuates diabetic nephropathy in streptozotocin-induced diabetic mice[J]. Mol Cell Endocrinol, 2012, 348(1): 104-11.
[4] 谢瑞奎,班博. 非酒精性脂肪性肝病与2型糖尿病及糖尿病肾病关系的研究进展[J]. 医学综述,2015,(1):107-108.
[5] 李风华. 鳖甲煎丸对肝纤维化大鼠的治疗作用及其对细胞因子p38、CTGF、TIMP-1及MMP-9表达的影响[D].石家庄:河北医科大学,2013.
[6] 罗俊华,巴元明. “肝肾同源”理论的研究进展[J]. 云南中医学院学报,2013,36(1): 91-93.
[7] 李光超,车念聪,耿建国,等. 基于“肝肾同源”探讨DN大鼠肝中TGF-β1、Smad-4、α-SMA和PDGF在中药治疗前后的变化[J]. 中国中医基础医学杂志,2014,(4): 459-461.
[8] 穆鑫,袁霞,康白,等. 糖尿病大鼠肾脏水通道蛋白-2mRNA的表达及黄芪的调节作用[J]. 中国药理学通报,2010,26(9):1176-1179.
[9] 王晓琳,翁孝刚,王宪华,等. 黄芪多糖预防2型糖尿病大鼠肝损伤的实验研究[J]. 现代生物医学进展,2016,16(10): 1846-1849.
[10] 杜宇琼,赵晖,张秋云,等. 水红花子对免疫肝纤维化大鼠肝功能及血清肝纤维化标志物的影响[J]. 中国中医急症,2011,(9): 1433-1434.
[11] 邹大威,高彦彬,刘迎新,等. 糖肾宁对自发性2型糖尿病kk-ay小鼠肾组织MMP-9、TIMP-1表达的影响[J]. 环球中医药,2015,8(11):1324-1328.
[12] 陈枫,杨爱萍,李燕,等. 糖尿病对大鼠肝纤维化组织TIMP-1/MMP-1表达的影响[J]. 重庆医学,2013,42(9): 964-967.
[13] 余毅,李增棋,陈昆,等. 乌司他丁对心脏联合瓣膜置换术患者S100β蛋白及早期认知功能障碍的影响[J]. 中国现代医生,2015,(14): 74-77.

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备注/Memo

备注/Memo:
基金项目: 国家重点基础研究发展计划(973计划)(2012CB518602)
作者简介:刘晔(1992- ),女,2015级在读硕士研究生。研究方向:中医肝病的证治规律研究。E-mail:liuye7562@163.com
通信作者:车念聪(1957- ),博士,教授,博士生导师,主任医师。研究方向:中医肝病。E-mail:cnc088@sohu.com
更新日期/Last Update: 2017-10-06