|本期目录/Table of Contents|

[1]赵琛,施茵,崔云华,等.温和灸肾俞穴、关元穴对肾阳虚老年人衰老症状及外周血Rb蛋白表达的影响[J].环球中医药,2012,5(05):350-0.
　ZHAO Chen,SHI Yin,CUI Yun hua,et al.Effect of suspended moxibustion on the pRb expression of peripheral blood in the kidneyyang deficiency aged people[J].,2012,5(05):350-0.
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温和灸肾俞穴、关元穴对肾阳虚老年人衰老症状及外周血Rb蛋白表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第5卷
期数:
2012年05期
页码:
350-0
栏目:
论著
出版日期:
2012-05-06

文章信息/Info

Title:
Effect of suspended moxibustion on the pRb expression of peripheral blood in the kidneyyang deficiency aged people
作者:
赵琛; 施茵; 崔云华; 王晓梅; 马晓芃; 吴蓓玲; 吴璐一; 刘慧荣;
Author(s):
ZHAO Chen;  SHI Yin;  CUI Yunhua; et al.
Acupuncture Department, Yueyang Hospital of Integrative Chinese & Western Medicine Affiliated to Shanghai University of TCM, Shanghai 200437, China
关键词:
温和灸;  衰老;  成视网膜母细胞瘤蛋白;  临床研究
Keywords:
Suspended moxibustion; Aging; Retinoblastoma protein; Clinical study
分类号:
R246
DOI:
-
文献标志码:
A
摘要:
目的观察温和灸对肾阳虚老年人外周血成视网膜母细胞瘤(retinoblastoma,Rb)蛋白表达以及衰老症状的影响,探讨温和灸的抗衰老作用。方法将60～75岁肾阳虚老年人81例随机分为温和灸治疗组41例和衰老对照组40例。温和灸治疗组采用温和灸肾俞穴、关元穴,18次为一个疗程,连续治疗两个疗程。采用积分表法观察温和灸对老年人治疗后综合情况的影响和症状改善情况,并用流式细胞术检测外周血Rb蛋白表达情况。结果 (1)经温和灸治疗,肾阳虚老年人衰老症状积分较衰老对照组疗效显著降低(P<0.01); (2)温和灸第二疗程末疗效较第一疗程末显著提高(P<0.01); (3)温和灸治疗组老年人外周血单个核细胞Rb蛋白阳性表达率显著低于衰老对照组(P<0.01)。结论温和灸可能通过干预肾阳虚老年人外周血Rb表达,改善衰老症状,降低衰老见证积分。
Abstract:
ObjectiveTo explore the antiaging effect of suspended moxibustion on the Rb expressions of the peripheral blood and general symptoms in aged people. MethodsA total of 81 elderly people were enrolled. The aged people between 6075 years old were randomly divided into two groups including 41 in suspended moxibustion group and 40 in aging control group. Suspended moxibustion on points Shenshu (BL23) and Guanyuan (CV4) were applied to the treated group under treatment for two courses. The effects of suspended moxibustion on aging symptom of aged people were observed with integral table.The total effective rate of suspended moxibustion was assessed by symptom scores, and the Rb expression of the peripheral blood was measured by flow cytometry. Results（1）Symptoms of aging integral of suspended moxibustion group was significantly reduced than that in the aging control group（P＜0.01）；(2) The effect of suspended moxibustion group in the second course was significantly improved than that in the first course（P＜0.01）.（3）The Rb expression rates of the peripheral blood in suspended moxibustion group were lower than that in the aging group（P＜0.01）. ConclusionThe antiaging effect of suspended moxibution may be through decreasing the pRb expression of the peripheral blood in aged people.

参考文献/References:

[1]肖达，陈汉平.温和灸对老年人衰老见证和T细胞亚群的影响[J].辽宁中医杂志，1996，23(12): 563564.
[2]丁菊英，赵粹英，吴恒举，等.温和灸对老年人红细胞免疫及自由基的影响[J].上海针灸杂志，1995，14(1):45.
[3]崔云华,施茵,张卫，等.艾灸对衰老大鼠肝组织细胞周期及PKC、PP2A表达的影响[J].上海针灸杂志,2008,27(6):4144.
[4]崔云华,施茵,国兰琴，等.艾灸对老年人衰老症状及外周血单个核细胞细胞周期的影响[J].辽宁中医杂志,2008,35(5):758760.
[5]中华人民共和国卫生部药政局.新药(中药)治疗老年病临床研究指导原则[J].中国医药学报，1989，4(3):232234.
[6]第三次全国中医、中西医结合老年医学研究协作组会议.延缓衰老中药的筛选规程和临床观察规范[J].中西医结合杂志，1986，6(11):682684.
[7]郦章安，吴福春.现代老年药学[M].北京:中国医药科技出版社，2001：493494.
[8]Zhao CY，Yang L，Chen HP，et al. Clinical Study on Antiaging Action of Herbal Cakepartition Moxibustion[J]. J Acupuncture Tuina Sci，2009，7（1）:3740.
[9]高希言,王燕.艾灸强壮要穴对衰老小鼠免疫功能的影响[J].河南中医,2005,25(11):2426.
[10]刘星,王红,高洪泉,等.针刺老年大鼠“足三里”、“关元”穴对心、肾GSHPX、LIP、NO含量影响的实验研究[J].中医药学报,2004,32(1):2223.
[11]李亚东,高洪泉,朱梅,等.针刺老年大鼠“足三里”“关元”穴对NO、SOD、MDA以及免疫影响的实验研究[J].中国针灸,2002,22(11):5254.
[12]Zhou EH，Liu HR，Wu HG，et al. Suspended moxibustion relieves chronic visceral hyperalgesia via serotonin pathway in the colon[J]. Neurosci Lett，2009，451(2):144147.
[13]宋丰军，郑士立，马大正针炙治疗排卵障碍性不孕症临床观察［Ｊ］.中国针炙，2008，28(1):2123.
[14]Deng Q，Li Y，Tedesco D，et al.The ability of E1A to rescue rasinduced premature senescence and confer transformation relies on inactivation of both p300/CBP and Rb family proteins[J].Cancer Res，2005，65(18):82988307.
[15]胡作为,周燕萍,沈自尹，等.p16基因与细胞衰老关系的研究进展[J].国外医学(遗传学分册),2004,27(4):200202.
[16]王枫,孙晓江.Rb基因与细胞衰老的研究进展[J].中国临床神经科学,2005,13(1):101105.
[17]韩广明，高晓兰，乔建勇,等.何首乌饮对衰老大鼠脑组织细胞Rb/p53信号转导通路影响［J］.河北医学，2011，17（1）：14.
[18]Schmid G，Kramer MP，Maurer M，et al.Cellular and organismal ageing: Role of the p53 tumor suppressor protein in the induction of transient and terminal senescence[J].J Cell Biochem，2007，101(6):13551369.
[19]童坦君，张宗玉.细胞衰老主导基因p16的作用机理及其负调控[J].中国生物化学与分子生物学报,2002,18(1):104.
[20]闫海龙，龚勇珍.氧化应激及p16和p53/p21与细胞衰老关系的研究进展[J].医学综述，2011，17（5）：682685.
[21]Klimova TA, Bell EL, Shroff EH，et al. Hyperoxiainduced premature senescence requires p53 and pRb，but not mitochondrial matrix ROS[J]. The FASEB Journal，2009，23（3）:783794.
[22]谭琳蓥,赵天平,国兰琴，等.温和灸对自然衰老模型大鼠肝组织Rb、P53、Bcl2、PKC表达的影响[J].环球中医药,2011,4(6):406412.

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备注/Memo

备注/Memo:
基金项目：国家重点基础研究发展计划（973计划)（2009CB522900）；国家自然科学基金（30701124）；上海市重点学科建设项目(S30304)
作者单位：200437上海中医药大学附属岳阳中西医结合医院针灸科（赵琛、刘慧荣）；上海中医药大学针灸免疫三级实验室（施茵、崔云华、王晓梅、马晓芃、吴蓓玲、吴璐一）
作者简介：赵琛（1973- ），女，博士，副主任医师。研究方向：针灸免疫研究。Email：chris_0127@126.com
更新日期/Last Update: 1900-01-01