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[1]黎发根,李绍旦,杨明会,等.和胃安神方对失眠模型大鼠血浆及下丘脑胆囊收缩素8的影响[J].环球中医药,2012,5(09):663-0.
　LI Fa gen,LI Shao dan,YANG Ming hui,et al.Effect of Hewei Anshen Decoction on cholecystokinin8 levels in plasma and hypothalamus of insomnic rats[J].,2012,5(09):663-0.
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和胃安神方对失眠模型大鼠血浆及下丘脑胆囊收缩素8的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第5卷
期数:
2012年09期
页码:
663-0
栏目:
论著
出版日期:
2012-09-06

文章信息/Info

Title:
Effect of Hewei Anshen Decoction on cholecystokinin8 levels in plasma and hypothalamus of insomnic rats
作者:
黎发根; 李绍旦; 杨明会; 周建平; 曹科; 刘毅;
Author(s):
LI Fagen; LI Shaodan; YANG Minghui; et al.
Institute of Traditional Chinese Medicine,Chinese PLA General Hospital, Beijing 100853,China
关键词:
和胃安神方;  失眠;  血浆;  下丘脑;  胆囊收缩素8
Keywords:
Hewei Anshen Decoction; Insomnia; Plasma; Hypothalamus; Cholecystokinin8
分类号:
R285.5
DOI:
-
文献标志码:
A
摘要:
目的探讨和胃安神方对失眠大鼠血浆及下丘脑胆囊收缩素8(CCK-8)的影响。方法将30只雄性wistar大鼠随机分为正常对照组、失眠模型组、和胃安神方中药组。采用放射免疫分析法检测血浆及下丘脑组织CCK-8的含量。结果和胃安神方中药组血浆CCK-8含量为(19.320±4.577)pg/ml,高于模型组(12.924±5.132)pg/ml,差异有统计学意义(P<0.05),与正常组(23.718±6.917)pg/ml比较无显著性差异(P>0.05); 和胃安神方中药组下丘脑CCK-8含量为(1.655±0.415)pg/mg,高于模型组(1.058±0.398)pg/mg,差异有统计学意义(P<0.05),与正常组(1.936±0.652)pg/mg比较无显著性差异(P>0.05)。结论和胃安神方治疗失眠症的疗效机制可能与其上调CCK-8水平有关。
Abstract:
ObjectiveTo study the effect of Hewei Anshen Decoction(HWASD) on CCK8 levels in plasma and hypothalamus of insomnic rats.MethodsThirty healthy male wistar rats were randomly divided into three groups:normal control group,insomnia model group and HWASD treatment group.Radioimmunoassav was used to measure the levels of CCK8 in plasma and hypothalamus. ResultsThe concentration of CCK8 in the plasma of HWASD treatment group was (19.320±4.577) pg/ml,significantly higher than that of insomnia model group (12.924±5.132) pg/ml (P<0.05),similar to normal control group (23.718±6.917) pg/ml (P>0.05).The level of CCK8 in the hypothalamus of HWASD treatment group was (1.655±0.415) pg/mg, significantly higher than that of insomnia model group(1.058±0.398) pg/mg (P<0.05), similar to normal control group (1.936±0.652) pg/mg(P>005). ConclusionThe therapeutic effect of Hewei Anshen Decoction on insomnia is partially attributed to increased content of CCK8.

参考文献/References:

[1]共识专家组.失眠定义、诊断及药物治疗专家共识(草案)[J].中华神经科杂志,2006,39(2):141143.
[2]罗海鸥,李绍旦,杨明会,等.和胃安神汤对失眠症患者睡眠及生活质量的影响[J].中国中医药信息杂志,2011,18(6):1820.
[3]张丽萍,夏猛.失眠症的治疗现状分析及思考[J].环球中医药,2011,4(1):6669.
[4]李绍旦,杨明会.和胃安神法治疗亚健康失眠的临床研究[J].中国全科医学,2010,13(22):25192520.
[5]肖成荣,马增春,李海静,等.PCPA失眠大鼠模型的制作及其机制[J].药理毒理学杂志,2007,21(4):326.
[6]余敏,唐成林,刘祖丽,等.不同强度电针刺激对失眠大鼠下丘脑orexinA的影响研究[J].医学分子生物学杂志,2011,8(1):7074.
[7]李绍旦,杨明会.不寐之中医“脑”、“胃”学说体系构建的理论初探[J].中医杂志,2010,51(2):2526.
[8]Hadjiivanova C，Belcheva S，Belcheva I．Cholecystokinin and learning and memory processes[J]．Aeta Physiol Pharmacol Buk,2003,27(23):83.
[9]李俊川,姜淮芜.胆囊收缩素临床研究进展[J].现代医药卫生,2012,28(3):407408.
[10]Crespi F. CholecystokininB (CCKB) receptor antagonists improve “aged” sleep: a new class of sleep modulators? [J].Methods and findings in experimental and clinical pharmacology,1999,21(1):3138.
[11]Shemyakin A， Kapás L. L364,718, a cholecystokininA receptor antagonist, suppresses feedinginduced sleep in rats[J].American journal of physiology. Regulatory, integrative and comparative physiology, 2001,280(5):R14201426.
[12]Kronenberg G，Schredl M，Fiedler K，et al. In healthy volunteers responses to challenge with cholecystokinin tetrapeptide differ between administration during REM and delta sleep[J].Depression and anxiety, 2001,14(2):141144.
[13]Levente Kapás, Ferenc Obál Jr, Mark R. Opp, et al.Intraperitoneal injection of cholecystokinin elicits sleep in rabbits[J]. Physiology & Behavior,1991,50(6):12411244.
[14]Marco Lanza，Francesco Makovec. Cholecystokinin (CCK) increases GABA release in the rat anterior nucleus accumbens via CCKB receptors located on glutamatergic interneurons[J].NaunynSchmiedeberg′s Arch Pharmacol，2000,361:3338.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金（81173429）;首都医学发展基金(SF2009Ⅱ16)
作者单位：100853 北京，中国人民解放军总医院中医研究所[黎发根(硕士研究生)、李绍旦、杨明会、曹科、刘毅]；军事医学科学院毒物药物研究所（周建平）
作者简介：黎发根（1985- ），2010级在读硕士研究生。研究方向：中西医结合老年病。Email：lifagen5@163.com
通讯作者：李绍旦（1976- ），博士，副主任医师。研究方向：中西医结合基础与临床。Email：lsd301@126.com
更新日期/Last Update: 1900-01-01