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[1]黎发根,李绍旦,杨明会,等.和胃安神方对失眠大鼠血浆及下丘脑食欲素A的影响[J].环球中医药,2012,5(11):801-0.
　LI Fa gen,LI Shao dan,YANG Ming hui,et al.Effect of Hewei Anshen Decoction on orexin A levels in plasma and hypothalamus of insomnic rats[J].,2012,5(11):801-0.
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和胃安神方对失眠大鼠血浆及下丘脑食欲素A的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第5卷
期数:
2012年11期
页码:
801-0
栏目:
论著
出版日期:
2012-11-06

文章信息/Info

Title:
Effect of Hewei Anshen Decoction on orexin A levels in plasma and hypothalamus of insomnic rats
作者:
黎发根; 李绍旦; 杨明会; 周建平; 曹科; 刘毅;
Author(s):
LI Fagen;  LI Shaodan;  YANG Minghui; et al.
Institute of Traditional Chinese Medicine,Chinese PLA General Hospital, Beijing 100853,China
关键词:
和胃安神方;  失眠;  血浆;  下丘脑;  食欲素A
Keywords:
Hewei Anshen Decoction; Insomnia; Plasma; Hypothalamus; Orexin A
分类号:
R285.5
DOI:
-
文献标志码:
A
摘要:
目的探讨和胃安神方对失眠大鼠血浆及下丘脑食欲素A(orexin A)的影响。方法将30只雄性wistar大鼠随机分为正常对照组、失眠模型组、和胃安神方中药组。除正常组外,其余两组大鼠均用对氯苯丙氨酸(PCPA)建立大鼠失眠模型。中药组给予和胃安神方水溶液10ml/kg灌胃,其余两组给予等量生理盐水灌胃。6天后处死并取血浆及下丘脑,采用用放射免疫分析法检测血浆及下丘脑组织orexinA的含量。采用单因素方差分析方法进行组间比较。结果和胃安神方中药组血浆orexinA含量为(22.35±4.33)pg/ml,模型组为(29.89±3.04)pg/ml,正常组为(21.28±4.02)pg/ml,中药组显著低于模型组,差异具有统计学意义(P<0.01),中药组与正常组比较无显著性差异(P>0.05); 和胃安神方中药组下丘脑orexinA含量为(21.34±2.94)pg/mg,模型组为(29.75±5.05)pg/mg,正常组为(18.84±3.08)pg/mg,中药组显著低于模型组,差异具有统计学意义(P<0.01),中药组与正常组比较无显著性差异(P>0.05)。结论和胃安神方治疗失眠症的疗效机制可能与其下调orexinA水平有关。
Abstract:
ObjectiveTo study the effect of Hewei Anshen Decoction(HWASD) on orexin A levels in plasma and hypothalamus in insomnic rats. MethodsThirty healthy male Wistar rats were randomly divided into three groups: normal control group, insomnia model group and HWASD treatment group. All rats but those in the normal control group were intraperitoneally injected with PCPA to be made into rat models of insomnia. The HWASD group were fed with Hewei Anshen Decoction(10 ml/kg) for 6 days, the other two groups with normal saline. After that, their plasma were collected, and hypothalamus were removed. Radioimmunoassav was used to measure the levels of orexin A in plasma and hypothalamus. ResultsThe concentration of orexin A in the plasma of HWASD treatment group was (22.35±4.33)pg/ml, significantly lower than that of insomnia model group(P<0.01), similar with normal control group(P>0.05). The levels of orexin A in the hypothalamus of HWASD treatment group was (2134±2.94)pg/mg, significantly lower than that of insomnia model group(P<0.01), similar with normal control group(P>0.05). ConclusionThe therapeutic effect of Hewei Anshen Decoction on insomnia is partially attributed to decreased content of orexin A.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金（81173429）;首都医学发展基金(SF2009Ⅱ16)
作者单位：100853 北京，解放军总医院中医研究所[黎发根(硕士研究生)、李绍旦、杨明会、曹科、刘毅]；军事医学科学院毒物药物研究所（周建平）
作者简介：黎发根（1985- ），2010级在读硕士研究生。研究方向：中西医结合老年病。Email：lifagen5@163.com
通讯作者：李绍旦（1976- ），博士，副主任医师。研究方向：中西医结合基础与临床。Email：lsd301@126.com
更新日期/Last Update: 1900-01-01