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[1]郑德松,李旗,田福玲,等.消毒愈肌膏对大鼠糖尿病足溃疡组织BMP-9、TGF-β1表达的影响[J].环球中医药,2013,6(07):485-0.
　ZHENG De song,LI Qi,TIAN Fu ling,et al.Analysis on effects of Xiaodu Yuji Paste on of the ulcer diabetic feet by BMP9, TGFβ1 gene expression[J].,2013,6(07):485-0.
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消毒愈肌膏对大鼠糖尿病足溃疡组织BMP-9、TGF-β1表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第6卷
期数:
2013年07期
页码:
485-0
栏目:
论著
出版日期:
2013-07-06

文章信息/Info

Title:
Analysis on effects of Xiaodu Yuji Paste on of the ulcer diabetic feet by BMP9, TGFβ1 gene expression
作者:
郑德松;李旗;田福玲;刘国荣;陈金铭;马树祥;崔建美;王洪彬;李雪青;
Author(s):
ZHENG Desong;  LI Qi;  TIAN Fuling;  et al.
College of TCM, Heibei Unite University, Tangshan 063000, China
关键词:
消毒愈肌膏; 糖尿病足; 骨形态发生蛋白-9; 转化生长因子β1
Keywords:
Xiaodu Yuji Paste; Diabetic foot; BMP9; TGFβ1
分类号:
R5872
DOI:
-
文献标志码:
A
摘要:
目的观察消毒愈肌膏对大鼠糖尿病足溃疡组织骨形态发生蛋白-9(bonemorphogeneticprotein-9,BMP-9)和转化生长因子β1(transforminggrowthfactor-β1,TGF-β1)表达的影响。方法选用链脲佐菌-素腹腔注射与薄铁片固定法制备模型,按随机数字表法分为中药组、模型组、BMP-9对照组和TGF-β1对照组,每组20只,并将大鼠足部溃疡处分别外敷消毒愈肌膏和无菌盐水,腹腔注射外源性BMP-9、TGF-β1,30天后取四组溃疡组织标本,采用miRCU-RYTM芯片检测各组基因并分析基因表达。结果BMP-9对照组和中药组中BMP-9基因表达明显增加,与模型组相比有显著差异(P≤0.01),BMP-9对照组与中药组BMP-9基因相比有显著差异(P≤0.05);TGF-β1对照组和中药组TGF-β1基因表达明显增高,与模型组相比有显著差异(P≤0.01),TGF-β1对照组与中药组相比,TGF-β1基因表达无明显差异(P≥0.05),中药组中BMP-9和TGF-β1表达变化呈显著正相关。结论消毒愈肌膏能显著调控BMP-9、TGF-β1表达水平,间接刺激组织细胞的分裂增殖,以利于创伤修复。
Abstract:
ObjectiveTo investigate the effects of Xiaodu Yuji Paste on treatment of diabetic feet from the level of genome. Methods 80 rats of diabetic foot ulcer were randomly divided into four groups (20 each) to receive traditional Chinese medicine, intraperitoneal injection of exogenous BMP9, TGFβ1 and saline for 30 days. Total RNA was extracted from the tissue collected near the wound, and the expression profile of gens demonstrated using the miRCURYTM for rats. Results The levels of serum BMP9 in BMP9 group and TCM group were significantly higher than those in model group . The levels of serum BMP9 in TCM group were significantly higher than those in BMP9 group. The levels of serum TGFβ1 in TGFβ1 group and TCM group were significantly higher than those in model group. There are not difference in the levels of serum TGFβ1 in TGFβ1 group and TCM group. The levels of serum BMP9 and TGFβ1are significantly positively related to change in TCM group. ConclusionThese findings suggest that the Xiaodu Yuji Paste can regulate the differential expression of some genes. Indirect stimulation of cell proliferation, in order to facilitate wound healing.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：唐山市科技计划(121302118b)
作者单位：063000 唐山，河北联合大学附属医院康复科(郑德松)；河北联合大学中医学院(李旗、田福玲、马树祥、崔建美、王洪彬、李雪青)，校医院(刘国荣)，中药学院(陈金铭)
作者简介：郑德松(1982- )，硕士，主治医师。研究方向：中西医结合内分泌系统疾病。Email: 58761595@qq.com
通讯作者：李旗(1980- )，硕士，讲师。研究方向：中西医结合内分泌系统疾病。Email: liqi19801211@163.com
更新日期/Last Update: 1900-01-01