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[1]李光超 车念聪 耿建国等.加味消渴康对糖尿病肾病大鼠肝组织细胞外基质相关 调节因子表达的影响[J].环球中医药,2014,7(02):88-0.
　LI Guang chao,CHE Nian cong,GENG Jian guo,et al.The effects of JiaweiXiaokekang on the expressions of factors regulating the hepatic extracellular matrix in DN rats[J].,2014,7(02):88-0.
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加味消渴康对糖尿病肾病大鼠肝组织细胞外基质相关 调节因子表达的影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第7卷
期数:
2014年02期
页码:
88-0
栏目:
论著
出版日期:
2014-02-06

文章信息/Info

Title:
The effects of JiaweiXiaokekang on the expressions of factors regulating the hepatic extracellular matrix in DN rats
作者:
李光超 车念聪 耿建国等
Author(s):
LI Guangchao; CHE Niancong; GENG Jianguo; et al.
College of traditonal Chinese medicine，Capital Medical University，Beijing 100069，China
关键词:
肝肾同源; 糖尿病肾病; 肝损伤; 转化生长因子β1; 基质金属蛋白酶9; 金属蛋白酶组织抑制剂1
Keywords:
Homogeny of liver and kidney;  Diabetic nephropathy;  Hepatic injury;  Transforming growth factorβ1;  Matrix metalloproteinase9;  Tissue inhibitor of metalloproteinases1
分类号:
R2855
DOI:
-
文献标志码:
A
摘要:
目的基于中医“肝肾同源”理论，观察加味消渴康对糖尿病肾病大鼠肝组织转化生长因子β1(transforming growth factorβ1，TGFβ1)，Smad4蛋白(drosophila mothers against decapentaplegic protein4)，基质金属蛋白酶9(matrix metalloproteinase9，MMP9)和金属蛋白酶组织抑制剂1（tissue inhibitor of metalloproteinases1，TIMP1）表达的影响。方法采用单侧肾切除加链脲佐菌素诱导的糖尿病肾病模型，将造模成功后的50只糖尿病肾病大鼠随机分为模型组、西药组和中药小、中、大剂量组，每组10只，另选取10只正常大鼠作为正常对照。其中西药组予氯沙坦(52 mg/kg·d)灌胃，中药组予加味消渴康灌胃(小、中、大剂量分别为13125 g/kg·d，26.25 g/kg·d和525 g/kg·d)，正常组及模型组每日灌服2 ml蒸馏水，共灌胃12周。各组大鼠于第12周末处死取出肝脏，运用ELisa法观察大鼠肝组织TGFβ1、Smad4、MMP9和TIMP1的表达。结果与正常组相比，各组大鼠肝中TGFβ1，Smad4和TIMP1表达明显升高，而MMP9的表达明显降低，差异具有统计学意义（P＜005或P＜001），中药治疗后，各组大鼠TGFβ1，Smad4的表达与模型组相比明显降低，差异具有统计学意义（P＜0.01），而TIMP1和MMP9的表达则分别具有降低和升高的趋势。结论加味消渴康对DN大鼠的肝损伤具有一定的保护作用。
Abstract:
ObjectiveTo investigate the influence of JiaweiXiaokekang on the expression of TGFβ1, Smad4, MMP9 and TIMP1 in the hepatic tissues of diabetic nephropathy (DN) rats based on the "theory of homogeny of liver and kidney". MethodsThe rat model was established by uninephrectomy and injection of streptozotocin. 50 models were divided into 5 groups: model group，western medicine group and Chinese herb medicine groups including lowdose，medialdose and highdose group. There were 10 models in each group. Besides, another 10 normal rats were chosen as the normal group. Western medicine group was given losartan(5.2 mg/kg·d) by intragastric administration；Chinese herb medicine gro ups were given JiaweiXiaokekang(lowdose group:13.125 g/kg·d，medialdose group:26.25 g/kg·d and highdose group:52.5 g/kg·d1) by intragastric administration； The normal group and model group were fed with 2 ml distilled water daily. After 12 weeks, sacrificed all the rats and removed their hepars and then observed the expressions of TGFβ1, Smad4, MMP9 and TIMP1 by immunohistochemistry. ResultsCompared with the normal group，the expression of TGFβ1，Smad4 and TIMP1 in other groups significantly increased，while the expression of MMP9 significantly decreased (P＜005 or P＜001). After the treatment，compared with the model group，the expression of TGFβ1 and Smad4 in other groups significantly decreased. While there was a decreased or increased trend on the expression of TIMP1 and MMP9 respectively. ConclusionJiaweiXiaokekang had a certain protective effect on the hepatic injury of DN rats.

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备注/Memo

备注/Memo:
基金项目：首都中医药与护理研究专项（12ZHY02）
作者单位：100069首都医科大学中医药学院［李光超(硕士研究生)、车念聪、耿建国、郭新新、赵晖、白辰(硕士研究生)］；北京市平谷区中医医院（王志强）作者简介：李光超（1986-），2011级在读硕士研究生。研究方向：中医肝病的证治规律研究。Email：li252530538@163.com通讯作者：车念聪（1957-），本科，教授。研究方向：中医肝病的证治规律研究。Email：cnc088@sohu.com
更新日期/Last Update: 1900-01-01