[1]刘圆圆 黄新 谢赛华等.广西虎纹捕鸟蛛毒成分的氨基酸测定及
红外光谱定性分析[J].环球中医药,2014,7(02):100-0.
LIU Yuan yuan,HUANG Xin,XIE Sai hua,et al.Amino acid assay of toxin from the spider Guangxi Selecnocosmia Huwena and qualitative analysis by infrared spectrometry[J].,2014,7(02):100-0.
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广西虎纹捕鸟蛛毒成分的氨基酸测定及
红外光谱定性分析(
)
《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]
- 卷:
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第7卷
- 期数:
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2014年02期
- 页码:
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100-0
- 栏目:
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论著
- 出版日期:
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2014-02-06
文章信息/Info
- Title:
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Amino acid assay of toxin from the spider Guangxi Selecnocosmia Huwena and qualitative analysis by infrared spectrometry
- 作者:
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刘圆圆 黄新 谢赛华等
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- Author(s):
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LIU Yuanyuan; HUANG Xin; XIE Saihua; et al.
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Guilin Traditional Chinese Medical Hospital,Guilin 541002,China
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- 关键词:
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虎纹蛛毒; 氨基酸测定; 红外光谱
- Keywords:
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Selenocosmia Huwena toxin; Amino acids assay; Infrared spectrometry
- 分类号:
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R284.1
- DOI:
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-
- 文献标志码:
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A
- 摘要:
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目的通过质量分析的方法初步鉴定蛛毒的毒素化学成分。方法采用氨基酸自动分析仪对蛛毒的多种氨基酸成分进行定性及定量的研究,并且辅以红外光谱法进行定性研究。结果通过氨基酸测定法从虎纹捕鸟蛛中鉴定到谷氨酸、胱氨酸、赖氨酸含量较多,分别为815%、786%、764%,氨基酸总量为5593%;从样品粉末的红外光谱可知:该化合物可能含CH,OH,NH,CONH,苯环等主要氨基酸基团。结论结果表明该鉴别方法操作简便、专属性强,可用于蛛毒的定性鉴别和含量测定,初步建立了虎纹蛛毒成分的定性鉴定研究方法。
- Abstract:
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ObjectivePreliminary identification of the chemical composition of toxin from the spider Guangxi Selenocosmia Huwena with the method of quality analysis. MethodsAutomatic amino acid analyzer on a variety of amino acid composition of spider poisons was adopted to perform a qualitative as well as quantitative research, and the former was supplemented by infrared spectrometry . ResultsBy using amino acids analysis the chemical composition of Guangxi Selenocosmia Huwena toxin was identified, and the finding was the spider Guangxi Selenocosmia Huwena contains higher levels of glutamic acid, cystine, lysine, which were 8.15%, 7.86%,and 7.64%, respectively, and the total amino acids was 55.93%. The infrared spectrometry showed that the sample powder may containCH,OH, NH,CONH, benzene ring and other major amino group. ConclusionThe results showed that the identification method, being simple, and specific, could be used for spider poison qualitative identification and determination, and the specific approaches for such method was tentatively established.
参考文献/References:
[1]王家福,彭贤锦,谢莉萍.我国南方捕鸟蛛一新种(蜘蛛目:捕鸟蛛科)[J].湖南师范大学自然科学学报,1993,16(1):72.
[2]潘红平,蒙健宗,徐蕴丽,等.虎纹捕鸟蛛毒素的提取及毒力的测定[J].广西农业生物科学,1999,18(4):274277.
[3]周鸿雁,毛海峰,陈嘉勤,等.虎纹镇痛肽对脑缺血损伤大鼠神经保护作用的研究[J].湖南文理学院学报(自然科学版),2006,18(3):5052.
[4]李顺意,梁松平.虎纹捕鸟蛛毒液中透明质酸酶的纯化和部分性质的研究[J].生物化学杂志,1995,11(2):155160.
[5]李发美.分析化学[M].北京:人民卫生出版社,2003:252272.
相似文献/References:
备注/Memo
- 备注/Memo:
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基金项目:广西壮族自治区中医药管理局中医药科技专项(GZKZ10056)
作者单位:541002桂林市中医院药剂科(刘圆圆、谢赛华、谭芳);广西中医药大学附属瑞康医院神经外科(黄新、张峥峥、刘磊)作者简介:刘圆圆(1985-),女,硕士,药师。研究方向:药物分析。Email:275189113@qq.com通讯作者:黄新(1960-),本科,教授。研究方向:脑肿瘤手术治疗。Email:nnhuangxin@163.com
更新日期/Last Update:
1900-01-01