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[1]朱小香,郑淑霞,萨喆燕,等.胃黏膜损伤大鼠胃组织P2X1、P2X3和P2X5的表达及电针对其影响[J].环球中医药,2015,8(01):33-36.[doi:10.3969/j.issn.1674-1749.2015.01.009]
　ZHU Xiao-xiang,ZHENG Shu-xia,SA Zhe-yan,et al.The expression of P2X in the gastric tissue of rats with gastric mucosal injury and the effect of electro-acupuncture on it[J].,2015,8(01):33-36.[doi:10.3969/j.issn.1674-1749.2015.01.009]
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胃黏膜损伤大鼠胃组织P2X1、P2X3和P2X5的表达及电针对其影响()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年01期
页码:
33-36
栏目:
论著
出版日期:
2015-01-06

文章信息/Info

Title:
The expression of P2X in the gastric tissue of rats with gastric mucosal injury and the effect of electro-acupuncture on it
作者:
朱小香; 郑淑霞; 萨喆燕; 修春英; 董亚琴; 许金森
350003 福建省中医药研究院(朱小香、郑淑霞、萨喆燕、修春英、董亚琴、许金森)
Author(s):
ZHU Xiao-xiang;  ZHENG Shu-xia;  SA Zhe-yan;  et al.
Fujian Academy of TCM and Pharmacy, Fuzhou 350003, China Corresponding author: XU Jin-sen, E-mail: xujinsenjls@163.com
关键词:
胃黏膜应激损伤;  电针;  足三里;  P2X
Keywords:
Stress-induced gastric mucosal injury;  Electro-acupuncture;  Zusanli;  P2X
分类号:
R245.9+7
DOI:
10.3969/j.issn.1674-1749.2015.01.009
文献标志码:
A
摘要:
目的 观察电针足三里穴对胃黏膜应激损伤大鼠胃组织中P2X1、P2X3、P2X5 mRNA表达的影响,探讨电针足三里穴防治胃黏膜损伤的分子机制。方法 将SD大鼠随机分为正常对照组(正常组)、胃黏膜应激损伤模型组(模型组)和电针足三里穴组(电针组)。采用冷-束缚法制作胃黏膜应激损伤大鼠模型。造模后次日起电针组每天电针足三里穴20分钟,连续三天。RT-PCR法检测胃组织P2X1、P2X3、P2X5 mRNA表达。结果 各组大鼠均表达P2X1、P2X3、P2X5 mRNA; 模型组与正常组比较,P2X1 mRNA表达显著降低(P<0.01); 电针组与模型组比较,P2X1 mRNA表达显著升高(P<0.05); P2X3、P2X5 mRNA组间未见显著差异; 正常组大鼠胃组织P2X3和P2X5 呈显著正相关(P<0.05)。结论 胃黏膜应激损伤大鼠的胃组织P2X1 mRNA表达下降,电针足三里穴可以上调胃黏膜应激损伤大鼠胃组织P2X1 mRNA的表达。
Abstract:
Objective To observe the effect of electro-acupuncture(EA)at Zusanli on the expression of P2X1, P2X3 and P2X5 mRNA in the gastric tissue of rats with stress-induced gastric mucosal injury and explore the molecular mechanism for the prevention and treatment of gastric mucosal injury by means of EA at Zusanli.Methods SD rats were randomly divided into three groups:normal control group(Normal Group), stress-induced gastric mucosal injury group(Model Group)and EA at Zusanli group(EA Group). The rat model with stress-induced gastric mucosal injury was constructed by cold-restraint method. From the next day after modeling, EA Group received EA at Zusanli 20 minutes a day for three consecutive days. The expression of P2X1, P2X3 and P2X5 mRNA in the gastric tissue were tested by RT-PCR.Results The expression of P2X1, P2X3 and P2X5 mRNA in the gastric tissue of rats were observed in all the groups. Compared with the Normal Group, the expression of P2X1 mRNA in the gastric tissue in the Model Group was significantly decreased(P<0.01). Compared with the Model Group, the expression of P2X1 mRNA in the gastric tissue in the EA Group was significantly increased(P<0.05 ). There was no significant difference among groups in terms of P2X3 and P2X5. There was a significant positive correlation between P2X3 and P2X5 in the gastric tissue of rats in the Normal Group(P<0.05).Conclusion The expression of P2X1 mRNA in the gastric tissue of rats with stress-induced gastric mucosal injury was decreased, EA at Zusanli could up-regulate the expression of P2X1 mRNA in the gastric tissue of rats with stress-induced gastric mucosal injury.

参考文献/References:

[1] 黎喜平, 严洁. 针灸对胃粘膜损伤保护作用机制的研究进展[J]. 针刺研究, 2005, 30(1): 60- 63.
[2] 刘涌. 电针足三里对应激性胃粘膜损伤的保护作用[J]. 安徽中医学院学报, 2000, 19(2): 27-30.
[3] 朱舜丽,许冠荪,陈金珠,等. 电针足三里穴对应激性胃溃疡大鼠一氧化氮和儿茶酚胺的影响[J]. 中国中西医结合脾胃杂志, 1996, 4(1): 39.
[4] 李永刚, 钟甘平, 岳中谨, 等. 部分膀胱出口梗阻后 P2X1 嘌呤受体在膀胱的表达[J]. 第四军医大学学报, 2005, 26(10): 878- 880.
[5] 高显奎, 余跃. P2嘌呤受体与胃肠运动的研究进展[J]. 临床消化病杂志, 2009, 21(5): 315-317.
[6] 黄碧兰, 余良主, 刘寿仙. 大鼠孤束核微量注射 γ-氨基丁酸对电针抗应激性胃黏膜损伤的影响[J]. 中国中西医结合消化杂志, 2011(6): 381-384.
[7] 李忠仁. 实验针灸学 [M]. 北京: 中国中医药出版社, 2007: 255.
[8] Valera S, Hussy N, Evans R J, et al. A new class of ligand-gated ion channel defined by P2x receptor for extracellular ATP[J]. Nature, 1994(6497): 516-519.
[9] Fountain S J, Parkinson K, Young M T, et al. An intracellular P2X receptor required for osmoregulation in Dictyostelium discoideum[J]. Nature, 2007, 448(7150): 200-203.
[10] Burnstock G, King B F. Numbering of cloned P2 purinoceptors[J]. Drug development research, 1996, 38(1): 67-71.
[11] Nicke A, Bäumert H G, Rettinger J, et al. P2X1 and P2X3 receptors form stable trimers: a novel structural motif of ligand‐gated ion channels[J]. The EMBO Journal, 1998, 17(11): 3016-3028.
[12] Stoop R, Thomas S, Rassendren F, et al. Contribution of individual subunits to the multimeric P2X2 receptor: estimates based on methanethiosulfonate block at T336C[J]. Molecular pharmacology, 1999, 56(5): 973- 981.
[13] BA O, AH K, TK C, et al. A quantitative analysis of purinoceptor expression in human fetal and adult bladders[J]. The JOURNAL OF UROLOGY, 2001, 165(5): 1730-1734.
[14] 杨昕, 宋波, 方强,等. P2x 受体亚型在大鼠逼尿肌不稳定中的作用研究[J]. 第三军医大学学报, 2004, 26(8): 710-713.
[15] 聂坤. P2X 受体, ATP 和针刺的信号转换[J]. 上海针灸杂志, 2009, 28(5): 298-300.
[16] 陈鸣. 大鼠脑创伤后嘌呤受体 P2X1 亚型表达的研究[D].上海:第二军医大学, 2005.
[17] 李家增, 侯明, 包承鑫. 血小板疾病[M]. 北京: 科学技术文献出版社, 2009: 237-238.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金青年基金(81403490); 福建省卫生厅青年科研基金(2011-1- 42) 作者简介:朱小香(1980- ),女,硕士,实验师。研究方向:针灸经络研究。E-mail:zxx9@163.com 通讯作者:许金森(1963- ),硕士,研究员,硕士生导师。研究方向:针灸经络研究。E-mail:xujinsenjls@163.com
更新日期/Last Update: 2015-01-05