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[1]张微,刘甜甜,祖承哲,等.希望I号液对大鼠吸入臭氧肺损伤的保护作用研究[J].环球中医药,2015,8(02):153-157.[doi:10.3969/j.issn.1674-1749.2015.02.007]
　ZHANG Wei,LIU Tian-tian,ZU Cheng-zhe,et al.The protection effect of Hope I Decoction on ozone inhalation-induced injury in rats[J].,2015,8(02):153-157.[doi:10.3969/j.issn.1674-1749.2015.02.007]
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希望I号液对大鼠吸入臭氧肺损伤的保护作用研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年02期
页码:
153-157
栏目:
论著
出版日期:
2015-02-06

文章信息/Info

Title:
The protection effect of Hope I Decoction on ozone inhalation-induced injury in rats
作者:
张微; 刘甜甜; 祖承哲; 彭金娥; 冯丽莉; 王湘; 张燕; 蔡大勇; 王玥琦
100029 北京中医药大学基础医学院[张微(硕士研究生)、刘甜甜(硕士研究生)、彭金娥(硕士研究生)、冯丽莉、王湘、张燕、王玥琦]; 中国医学科学院药用植物研究所(祖承哲、蔡大勇)
Author(s):
ZHANG Wei;  LIU Tian-tian;  ZU Cheng-zhe;  et al.
School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China Corresponding author:Yue-Qi Wang, E-mail: yueqiwng@gmail.com
关键词:
希望I号液;  臭氧;  抗氧化;  肺损伤;  肺表面活性蛋白A
Keywords:
Hope I Decoction;  Ozone;  Antioxidant;  Lung injury;  Surfactant protein A
分类号:
R285.5
DOI:
10.3969/j.issn.1674-1749.2015.02.007
文献标志码:
A
摘要:
目的 观察希望I号液对大鼠吸入臭氧导致肺损伤的保护作用。方法 48只雄性SD大鼠随机分为6组,即对照组、模型组、维生素E组(0.0135 g/kg)、希望I号液低、中、高剂量组(0.045、0.090、0.180g/kg),除对照组其余各组暴露臭氧(1.2±0.2 ppm)10 h/d×45 d。维生素E组、希望I号液低、中、高剂量组在臭氧造模同时给药。检测血清中超氧化物歧化酶(superoxide dismutase, SOD)、过氧化脂质(lipid peroxide, LPO)、过氧化氢酶(catalase, CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)含量,HE、Masson染色观察肺组织变化,免疫组化方法观察肺组织中肺表面活性蛋白A含量。结果 与模型组相比,希望I号液各组SOD、CAT、GSH-Px活性增加,LPO水平降低; 希望I号液各给药组炎性细胞渗出胶原纤维含量减少; 希望I号液各组肺表面活性蛋白A含量明显高于模型组。结论 希望I号液对大鼠吸入臭氧导致损伤具有良好的保护作用。
Abstract:
Objective To evaluate the protection effect of Hope I decoction on ozone inhalation-induced injury in rats. Methods 48 male SD rats were randomly divided into 6 groups, i.e., control, model, VE(0.0135g/kg), Hope I decoction at three dosages(0.045, 0.090, 0.180g/kg respectively). Modeling rats were exposed to ozone in an exposure cabinet(10h/d×45d), then some of them were intragastrically administrated with vitamin E or Hope I decoction.Then, the level of SOD, LPO, CAT and GSH-Px in rat serum were detected and pathologic change of lung tissue were observed, Immunohistochemistry for surfactant protein A(SP-A)content were observed. Results The activity of SOD, CAT and GSH-Px in serum were increased and LPO level were reduced in all Hope I groups. Histopathological slides of these three groups also showed an attenuated extent of alveoli dilatation and collapse induced by ozone exposure, surfactant protein A of these three groups were significantly enhanced. Conclusion Hope I decoction has great protection effect on ozone inhalation-induced injury in rats.

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备注/Memo

备注/Memo:
基金项目:北京中医药大学科研创新团队项目(2011-CXD- 04); 北京中医药大学自主选题(2014-JYBZZ-XS- 036) 作者简介:张微(1989- ),女,2012级在读硕士研究生。研究方向:中药延缓衰老的作用及其机理研究。E-mail:sophiezw@163.com 通讯作者:王玥琦(1958- )女,博士,研究员。研究方向:中药延缓衰老的作用及其机理研究。E-mail:yueqiwng@gmail.com
更新日期/Last Update: 2015-02-20