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[1]王丽,李翌萌.慢性浅表性胃炎不同证型大鼠琥珀酸脱氢酶活性与细胞色素C含量对比研究[J].环球中医药,2015,8(03):313-315.[doi:10.3969/j.issn.1674-1749.2015.03.012]
　WANG Li,LI Yi-meng.Contrast of succinate dehydrogenase vitality and cytochrome C content in rats of different syndromes of chronic superficial gastritis[J].,2015,8(03):313-315.[doi:10.3969/j.issn.1674-1749.2015.03.012]
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慢性浅表性胃炎不同证型大鼠琥珀酸脱氢酶活性与细胞色素C含量对比研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第8卷
期数:
2015年03期
页码:
313-315
栏目:
论著
出版日期:
2015-03-06

文章信息/Info

Title:
Contrast of succinate dehydrogenase vitality and cytochrome C content in rats of different syndromes of chronic superficial gastritis
作者:
王丽; 李翌萌
110847 沈阳,辽宁中医药大学护理学院(王丽); 大连市第二人民医院消化科(李翌萌)
Author(s):
WANG Li;  LI Yi-meng
Nursing College of Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China Corresponding author: WANG Li,E-mail: wltcm@126.com
关键词:
湿热证;  脾虚证;  琥珀酸脱氢酶;  细胞色素C;  慢性浅表性胃炎
Keywords:
Damp-heat syndrome;  Spleen-deficiency syndrome;  Succinate dehydrogenase;  Chronic superficial gastritis
分类号:
R573.3+1
DOI:
10.3969/j.issn.1674-1749.2015.03.012
文献标志码:
A
摘要:
目的 探讨慢性浅表性胃炎(chronic superficial gastritis, CSG)脾虚与湿热证模型大鼠胃组织琥珀酸脱氢酶(succinate dehydrogenase,SDH)的活性及细胞色素C(cytochrome C,Cyt-C)的含量变化,从能量代谢的角度揭示CSG不同证候的实质。方法 用水杨酸钠溶液灌胃法复制大鼠单纯CSG模型,在此基础上,用小承气汤泻下法及饥饱失常法复制脾虚CSG模型; 用肥甘辛辣饮食法复制湿热CSG模型,5周后,检测大鼠胃组织琥珀酸脱氢酶活性及细胞色素C含量。结果 SDH的含量为:湿热CSG组最高,而脾虚CSG组最低。脾虚CSG组与湿热CSG组分别与其他组比较,差异均有统计学意义(P<0.05或P<0.01); 胞浆中Cyt-C含量造模组均高于正常组,差异有统计学意义(P<0.05或P<0.01),其中脾虚CSG组最高,湿热CSG组次之,两组之间比较,差异有统计学意义(P<0.01)。结论 CSG不同证型大鼠胃组织SDH活性及Cyt-C含量的差异,提示同一疾病不同证型之间能量代谢状态的不同,湿热证相对于脾虚证能量代谢较亢进。
Abstract:
Objective To explore the mechanisms of different syndromes in terms of the energy metabolism by observing the activity changes of succinate dehydrogenase(SDH)and the content changes of cytochrome C(Cyt-C)in rats with chronic superficial gastritis(CSG)of spleen-deficiency syndrome and damp-heat syndrome. Methods CSG rat model was established by the intragastric administration of sodium salicylate solution. CSG rat model with spleen-deficiency syndrome was established by Xiaochengqi Decoction-induced purgation and eating disorders. CSG rat model with damp-heat syndrome was established by feeding fat, sweet and spicy diet. Five weeks later, SDH activity and Cyt-C content of gastric tissue were determined. Results The activity of SDH in damp-heat CSG group was the highest of all, but which in spleen-deficiency CSG group was the lowest. The activity of SDH was obviously changed in damp-heat CSG group also in spleen-deficiency CSG group in comparison with the other three groups, respectively.(P<0.05 or P<0.01). The content of Cyt-C in the three model groups was higher than which in normal group(P<0.05 or P<0.01). The content of Cyt-C in spleen-deficiency CSG group was the highest of all, with the damp-heat CSG group secondarily. There was a significantly content difference between the two groups(P<0.01). Conclusion The difference in the SDH activity and Cyt-C content of CSG rat with different syndromes, revealed the different energy metabolism of different syndromes for the same disease, the energy metabolism of damp-heat symdrome was higher than which of spleen-deficiency syndrome.

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备注/Memo

备注/Memo:
作者简介:王丽(1978- ),女,博士,讲师。研究方向:中医消化病基础及临床研究。E-mail:wltcm@126.com
更新日期/Last Update: 2015-03-20