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[1]康丽 邱仁杰 王秀丽.沙苑子不同规格炮制品中沙苑子苷A 的含量比较研究[J].环球中医药,2017,10(06):578-581.[doi:10.3969/j.issn.1674-1749.2017.06.009]
　KANG Li,QIU Renjie,WANG Xiuli..Comparative study on the content of complanatuside A in different specifications of astragali complanati semen[J].,2017,10(06):578-581.[doi:10.3969/j.issn.1674-1749.2017.06.009]
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沙苑子不同规格炮制品中沙苑子苷A 的含量比较研究()
     
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《环球中医药》[ISSN:1006-6977/CN:61-1281/TN]

卷:
第10卷
期数:
2017年06期
页码:
578-581
栏目:
论著
出版日期:
2017-06-06

文章信息/Info

Title:
Comparative study on the content of complanatuside A in different specifications of astragali complanati semen
作者:
康丽 邱仁杰 王秀丽
719000 陕西省榆林市中医医院药学部(康丽); 北京中医药大学中药学院(邱仁杰、王秀丽)
Author(s):
KANG Li; QIU Renjie; WANG Xiuli.
Yulin Hospital of traditional Chinese medicine, Yulin 719000,China
关键词:
沙苑子;  炮制品;  沙苑子苷A;  高效液相色谱法;  含量测定
Keywords:
astragali complanati semen;  Processed products;  Complanatoside A;  HPLC;  Content determination
分类号:
R284
DOI:
10.3969/j.issn.1674-1749.2017.06.009
文献标志码:
A
摘要:
目的 建立沙苑子中沙苑子苷A的含量测定方法,考察沙苑子不同规格炮制品中沙苑子苷A的含量及其变化情况。方法 用盐水、黄酒、米醋三种辅料,按照烘、炒、蒸三种不同工艺,炮制出9种不同规格沙苑子炮制品,采用高效液相色谱(HPLC)法测定不同规格沙苑子中沙苑子苷A的含量。结果 沙苑子苷A在0.0209 μg～0.4180 μg范围内线性关系良好(r=0.999 9,n=5); 平均加样回收率为97.91%,RSD为1.48%,准确度符合要求。沙苑子生品、盐水闷润炒干品、盐水闷润烘干品、盐水闷润蒸后烘干品、黄酒闷润炒干品、黄酒闷润烘干品、黄酒闷润蒸后烘干品、米醋闷润炒干品、米醋闷润烘干品、米醋闷润蒸后烘干品中沙苑子苷A含量分别为0.98、0.89、0.85、1.16、0.88、0.90、1.09、0.85、0.87、1.18 mg/g。结论 建立的HPLC检测方法快速准确、重复性好、专属性强; 盐水、黄酒或米醋三种辅料闷润炒干和闷润烘干可导致沙苑子中沙苑子苷A含量下降,而辅料闷润蒸后烘干则使得沙苑子苷A含量增加,尤以米醋闷润蒸后烘干品中沙苑子苷A含量最高。
Abstract:
Objective To establish a method of content determination of complanatuside A in astragali complanati semen, and study the contents and changes of different specifications. Methods Saline water, rice-vinegar, rice-wine was used as excipients, according to the three different processes of baking, frying and steaming, the astragali complanati semen was made up of 9 kinds of products with different specifications, then the content of complanatuside A was determined by HPLC. Results Complanatuside A showed a good linearity relationship in the range of 0.0209 μg～0.4180 μg(r=0.999 9,n=5). The average recovery was 97.91%, RSD was 1.48%, the accuracy met the requirements. The contents of complanatuside A in not-processed product, roasting product after saline water moistening, drying product after saline water moistening, roasting product after saline water moistening and steaming, roasting product after rice-wine moistening, drying product after rice-wine moistening, roasting product after rice-wine moistening and steaming product, roasting product after rice-vinegar moistening, drying product after rice-vinegar moistening and drying product after rice-vinegar moistening and steaming, the content of complanatuside A was 0.98, 0.89, 0.85, 1.16, 0.88, 0.90, 1.09, 0.85, 0.87, 1.18 mg/g respectively. Conclusion The established HPLC detection method is fast, accurate, repeatable and strong specificity; roasting or drying after moistening with saline water, rice-vinegar or rice-wine can reduce the content of complanatuside A in astragali complanati semen, however the drying after moistening and steaming with excipients can increase the content of complanatoside A, the content of drying product after rice-vinegar moistening and steaming is highest.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 陕西省中医管理局中医药科研课题(15-ZY041); 国家中医药管理局全国中药特色技术传承人才培训项目
作者简介: 康丽(1972- ),女,本科,副主任,中药师。研究方向:中药鉴定。E-mail:1186825832@qq.com
通信作者:王秀丽(1978- ),女,博士,副研究员。研究方向:中药复方及新药研发。E-mail:Lnwangxiuli@163.com
更新日期/Last Update: 2017-06-06